Evaluation of a therapy for Idiopathic Chronic Enterocolitis in rhesus macaques (Macaca mulatta) and linked microbial community correlates
Idiopathic chronic enterocolitis (ICE) is one of the most commonly encountered and difficult to manage diseases of captive rhesus macaques (Macaca mulatta). The etiology is not well understood, but perturbations in gut microbial communities have been implicated. Here we evaluated the effects of a 14...
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doaj-7cfe6e3d6b9f4c3e84c2de0fb8b778ac2020-11-24T22:59:41ZengPeerJ Inc.PeerJ2167-83592018-04-016e461210.7717/peerj.4612Evaluation of a therapy for Idiopathic Chronic Enterocolitis in rhesus macaques (Macaca mulatta) and linked microbial community correlatesJoshua M. Taylor0Erik L. Clarke1Kate Baker2Abigail Lauder3Dorothy Kim4Aubrey Bailey5Gary D. Wu6Ronald G. Collman7Lara Doyle-Meyers8Kasi Russell-Lodrigue9James Blanchard10Frederic D. Bushman11Rudolf Bohm12Division of Veterinary Medicine, Tulane National Primate Research Center, Covington, LA, United States of AmericaDepartment of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA, United States of AmericaDivision of Veterinary Medicine, Tulane National Primate Research Center, Covington, LA, United States of AmericaDepartment of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA, United States of AmericaDivision of Gastroenterology, Hepatology, and Nutrition, The Children’s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, PA, United States of AmericaDepartment of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA, United States of AmericaDivision of Gastroenterology, Hepatology, and Nutrition, The Children’s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, PA, United States of AmericaDivision of Pulmonary, Allergy and Critical Care, Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, United States of AmericaDivision of Veterinary Medicine, Tulane National Primate Research Center, Covington, LA, United States of AmericaDivision of Veterinary Medicine, Tulane National Primate Research Center, Covington, LA, United States of AmericaDivision of Veterinary Medicine, Tulane National Primate Research Center, Covington, LA, United States of AmericaDepartment of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA, United States of AmericaDivision of Veterinary Medicine, Tulane National Primate Research Center, Covington, LA, United States of AmericaIdiopathic chronic enterocolitis (ICE) is one of the most commonly encountered and difficult to manage diseases of captive rhesus macaques (Macaca mulatta). The etiology is not well understood, but perturbations in gut microbial communities have been implicated. Here we evaluated the effects of a 14-day course of vancomycin, neomycin, and fluconazole on animals affected with ICE, comparing treated, untreated, and healthy animals. We performed microbiome analysis on duodenal and colonic mucosal samples and feces in order to probe bacterial and/or fungal taxa potentially associated with ICE. All treated animals showed a significant and long-lasting improvement in stool consistency over time when compared to untreated and healthy controls. Microbiome analysis revealed trends associating bacterial community composition with ICE, particularly lineages of the Lactobacillaceae family. Sequencing of DNA from macaque food biscuits revealed that fungal sequences recovered from stool were dominated by yeast-derived food additives; in contrast, bacteria in stool appeared to be authentic gut residents. In conclusion, while validation in larger cohorts is needed, the treatment described here was associated with significantly improved clinical signs; results suggested possible correlates of microbiome structure with disease, though no strong associations were detected between single microbes and ICE.https://peerj.com/articles/4612.pdfChronic colitis16S metagenomic sequencing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Joshua M. Taylor Erik L. Clarke Kate Baker Abigail Lauder Dorothy Kim Aubrey Bailey Gary D. Wu Ronald G. Collman Lara Doyle-Meyers Kasi Russell-Lodrigue James Blanchard Frederic D. Bushman Rudolf Bohm |
spellingShingle |
Joshua M. Taylor Erik L. Clarke Kate Baker Abigail Lauder Dorothy Kim Aubrey Bailey Gary D. Wu Ronald G. Collman Lara Doyle-Meyers Kasi Russell-Lodrigue James Blanchard Frederic D. Bushman Rudolf Bohm Evaluation of a therapy for Idiopathic Chronic Enterocolitis in rhesus macaques (Macaca mulatta) and linked microbial community correlates PeerJ Chronic colitis 16S metagenomic sequencing |
author_facet |
Joshua M. Taylor Erik L. Clarke Kate Baker Abigail Lauder Dorothy Kim Aubrey Bailey Gary D. Wu Ronald G. Collman Lara Doyle-Meyers Kasi Russell-Lodrigue James Blanchard Frederic D. Bushman Rudolf Bohm |
author_sort |
Joshua M. Taylor |
title |
Evaluation of a therapy for Idiopathic Chronic Enterocolitis in rhesus macaques (Macaca mulatta) and linked microbial community correlates |
title_short |
Evaluation of a therapy for Idiopathic Chronic Enterocolitis in rhesus macaques (Macaca mulatta) and linked microbial community correlates |
title_full |
Evaluation of a therapy for Idiopathic Chronic Enterocolitis in rhesus macaques (Macaca mulatta) and linked microbial community correlates |
title_fullStr |
Evaluation of a therapy for Idiopathic Chronic Enterocolitis in rhesus macaques (Macaca mulatta) and linked microbial community correlates |
title_full_unstemmed |
Evaluation of a therapy for Idiopathic Chronic Enterocolitis in rhesus macaques (Macaca mulatta) and linked microbial community correlates |
title_sort |
evaluation of a therapy for idiopathic chronic enterocolitis in rhesus macaques (macaca mulatta) and linked microbial community correlates |
publisher |
PeerJ Inc. |
series |
PeerJ |
issn |
2167-8359 |
publishDate |
2018-04-01 |
description |
Idiopathic chronic enterocolitis (ICE) is one of the most commonly encountered and difficult to manage diseases of captive rhesus macaques (Macaca mulatta). The etiology is not well understood, but perturbations in gut microbial communities have been implicated. Here we evaluated the effects of a 14-day course of vancomycin, neomycin, and fluconazole on animals affected with ICE, comparing treated, untreated, and healthy animals. We performed microbiome analysis on duodenal and colonic mucosal samples and feces in order to probe bacterial and/or fungal taxa potentially associated with ICE. All treated animals showed a significant and long-lasting improvement in stool consistency over time when compared to untreated and healthy controls. Microbiome analysis revealed trends associating bacterial community composition with ICE, particularly lineages of the Lactobacillaceae family. Sequencing of DNA from macaque food biscuits revealed that fungal sequences recovered from stool were dominated by yeast-derived food additives; in contrast, bacteria in stool appeared to be authentic gut residents. In conclusion, while validation in larger cohorts is needed, the treatment described here was associated with significantly improved clinical signs; results suggested possible correlates of microbiome structure with disease, though no strong associations were detected between single microbes and ICE. |
topic |
Chronic colitis 16S metagenomic sequencing |
url |
https://peerj.com/articles/4612.pdf |
work_keys_str_mv |
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