Characterization of 10 New Nuclear Microsatellite Markers in <i>Acca sellowiana</i> (Myrtaceae)
Premise of the study: Microsatellite primers were identified and characterized in <i>Acca sellowiana</i> in order to expand the limited number of pre-existing polymorphic markers for use in population genetic studies for conservation, phylogeography, breeding, and domestication. Methods...
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doaj-7d0b07ae45a94c4f9b69400d848994962020-11-25T00:32:13ZengWileyApplications in Plant Sciences2168-04502014-06-0126140002010.3732/apps.1400020Characterization of 10 New Nuclear Microsatellite Markers in <i>Acca sellowiana</i> (Myrtaceae)Gustavo H. F. Klabunde0Denise Olkoski1Vinicius Vilperte2Maria I. Zucchi3Rubens O. Nodari4Post Graduation Program in Plant Genetic Resources, Universidade Federal de Santa Catarina, CP 476 88034-000 Florianópolis, BrazilPost Graduation Program in Plant Genetic Resources, Universidade Federal de Santa Catarina, CP 476 88034-000 Florianópolis, BrazilPost Graduation Program in Plant Genetic Resources, Universidade Federal de Santa Catarina, CP 476 88034-000 Florianópolis, BrazilPolo Centro Sul, Agência Paulista de Tecnologia dos Agronegócios, CP 13400 970 Piracicaba, BrazilPost Graduation Program in Plant Genetic Resources, Universidade Federal de Santa Catarina, CP 476 88034-000 Florianópolis, BrazilPremise of the study: Microsatellite primers were identified and characterized in <i>Acca sellowiana</i> in order to expand the limited number of pre-existing polymorphic markers for use in population genetic studies for conservation, phylogeography, breeding, and domestication. Methods and Results: A total of 10 polymorphic microsatellite primers were designed from clones obtained from a simple sequence repeat (SSR)–enriched genomic library. The primers amplified di- and trinucleotide repeats with four to 27 alleles per locus. In all tested populations, the observed heterozygosity ranged from 0.269 to 1.0. Conclusions: These new polymorphic SSR markers will allow future genetic studies to be denser, either for genetic structure characterization of natural populations or for studies involving genetic breeding and domestication process in <i>A. sellowiana</i>.http://www.bioone.org/doi/full/10.3732/apps.1400020Acca sellowianafeijoaPlinia caulifloraPlinia jaboticabaPlinia trunciflorapopulation genetics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gustavo H. F. Klabunde Denise Olkoski Vinicius Vilperte Maria I. Zucchi Rubens O. Nodari |
spellingShingle |
Gustavo H. F. Klabunde Denise Olkoski Vinicius Vilperte Maria I. Zucchi Rubens O. Nodari Characterization of 10 New Nuclear Microsatellite Markers in <i>Acca sellowiana</i> (Myrtaceae) Applications in Plant Sciences Acca sellowiana feijoa Plinia cauliflora Plinia jaboticaba Plinia trunciflora population genetics |
author_facet |
Gustavo H. F. Klabunde Denise Olkoski Vinicius Vilperte Maria I. Zucchi Rubens O. Nodari |
author_sort |
Gustavo H. F. Klabunde |
title |
Characterization of 10 New Nuclear Microsatellite Markers in <i>Acca sellowiana</i> (Myrtaceae) |
title_short |
Characterization of 10 New Nuclear Microsatellite Markers in <i>Acca sellowiana</i> (Myrtaceae) |
title_full |
Characterization of 10 New Nuclear Microsatellite Markers in <i>Acca sellowiana</i> (Myrtaceae) |
title_fullStr |
Characterization of 10 New Nuclear Microsatellite Markers in <i>Acca sellowiana</i> (Myrtaceae) |
title_full_unstemmed |
Characterization of 10 New Nuclear Microsatellite Markers in <i>Acca sellowiana</i> (Myrtaceae) |
title_sort |
characterization of 10 new nuclear microsatellite markers in <i>acca sellowiana</i> (myrtaceae) |
publisher |
Wiley |
series |
Applications in Plant Sciences |
issn |
2168-0450 |
publishDate |
2014-06-01 |
description |
Premise of the study: Microsatellite primers were identified and characterized in <i>Acca sellowiana</i> in order to expand the limited number of pre-existing polymorphic markers for use in population genetic studies for conservation, phylogeography, breeding, and domestication.
Methods and Results: A total of 10 polymorphic microsatellite primers were designed from clones obtained from a simple sequence repeat (SSR)–enriched genomic library. The primers amplified di- and trinucleotide repeats with four to 27 alleles per locus. In all tested populations, the observed heterozygosity ranged from 0.269 to 1.0.
Conclusions: These new polymorphic SSR markers will allow future genetic studies to be denser, either for genetic structure characterization of natural populations or for studies involving genetic breeding and domestication process in <i>A. sellowiana</i>. |
topic |
Acca sellowiana feijoa Plinia cauliflora Plinia jaboticaba Plinia trunciflora population genetics |
url |
http://www.bioone.org/doi/full/10.3732/apps.1400020 |
work_keys_str_mv |
AT gustavohfklabunde characterizationof10newnuclearmicrosatellitemarkersiniaccasellowianaimyrtaceae AT deniseolkoski characterizationof10newnuclearmicrosatellitemarkersiniaccasellowianaimyrtaceae AT viniciusvilperte characterizationof10newnuclearmicrosatellitemarkersiniaccasellowianaimyrtaceae AT mariaizucchi characterizationof10newnuclearmicrosatellitemarkersiniaccasellowianaimyrtaceae AT rubensonodari characterizationof10newnuclearmicrosatellitemarkersiniaccasellowianaimyrtaceae |
_version_ |
1725320251589001216 |