In vitro propagation of Securidaca longipedunculata (Fresen) from shoot tip: an endangered medicinal plant
Abstract Background Securidaca longipedunculata Fresen is an indigenous medicinal plant in Africa that has an important place in both traditional and modern medicine. This plant is endangered because of high seed dormancy, low germination rate, and over exploitation. Therefore, micropropagation meth...
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Online Access: | https://doi.org/10.1186/s43141-019-0017-0 |
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doaj-7d0d6859ffcc4ab4a54aad996e7942832021-01-24T12:42:26ZengSpringerOpenJournal of Genetic Engineering and Biotechnology2090-59202020-01-0118111010.1186/s43141-019-0017-0In vitro propagation of Securidaca longipedunculata (Fresen) from shoot tip: an endangered medicinal plantTeklebrihan Lijalem0Tileye Feyissa1Institute of Biotechnology, Addis Ababa UniversityInstitute of Biotechnology, Addis Ababa UniversityAbstract Background Securidaca longipedunculata Fresen is an indigenous medicinal plant in Africa that has an important place in both traditional and modern medicine. This plant is endangered because of high seed dormancy, low germination rate, and over exploitation. Therefore, micropropagation method is important to address these problems. The objective of this study is to develop a micropropagation protocol for S. longipedunculata from shoot tip explants. Results Among different Clorox concentrations, seeds sterilized with 10% Clorox for 10 min resulted in 85% decontamination and 80% germination. Among different media used to evaluate the rate of seed germination, seeds that were de-coated and transversally cut at the tip and cultured on basal MS medium resulted in 100% germination. The highest percentage of shoot initiation (87%) was obtained on MS medium containing 1.0 mg/l 6-Benzylaminopurine (BAP). The highest mean shoot number per explant (8.5 ± 0.69) was achieved on MS multiplication medium containing 1.5 mg/l BAP in combination with 0.1 mg/l Indole-3-butyric acid (IBA). The highest mean number of roots per explant (3.73 ± 0.69) was obtained on MS medium containing 2.0 mg/l Indole-3-acetic-acid (IAA). Among plantlets transferred to greenhouse, 60% survived after acclimatization. Conclusions This micropropagation protocol can be used for mass propagation of S. longipedunculata that contributes to its conservation and genetic improvement.https://doi.org/10.1186/s43141-019-0017-0MicropropagationPlant growth regulatorsSecuridaca longipedunculataShoot initiationShoot multiplication |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Teklebrihan Lijalem Tileye Feyissa |
spellingShingle |
Teklebrihan Lijalem Tileye Feyissa In vitro propagation of Securidaca longipedunculata (Fresen) from shoot tip: an endangered medicinal plant Journal of Genetic Engineering and Biotechnology Micropropagation Plant growth regulators Securidaca longipedunculata Shoot initiation Shoot multiplication |
author_facet |
Teklebrihan Lijalem Tileye Feyissa |
author_sort |
Teklebrihan Lijalem |
title |
In vitro propagation of Securidaca longipedunculata (Fresen) from shoot tip: an endangered medicinal plant |
title_short |
In vitro propagation of Securidaca longipedunculata (Fresen) from shoot tip: an endangered medicinal plant |
title_full |
In vitro propagation of Securidaca longipedunculata (Fresen) from shoot tip: an endangered medicinal plant |
title_fullStr |
In vitro propagation of Securidaca longipedunculata (Fresen) from shoot tip: an endangered medicinal plant |
title_full_unstemmed |
In vitro propagation of Securidaca longipedunculata (Fresen) from shoot tip: an endangered medicinal plant |
title_sort |
in vitro propagation of securidaca longipedunculata (fresen) from shoot tip: an endangered medicinal plant |
publisher |
SpringerOpen |
series |
Journal of Genetic Engineering and Biotechnology |
issn |
2090-5920 |
publishDate |
2020-01-01 |
description |
Abstract Background Securidaca longipedunculata Fresen is an indigenous medicinal plant in Africa that has an important place in both traditional and modern medicine. This plant is endangered because of high seed dormancy, low germination rate, and over exploitation. Therefore, micropropagation method is important to address these problems. The objective of this study is to develop a micropropagation protocol for S. longipedunculata from shoot tip explants. Results Among different Clorox concentrations, seeds sterilized with 10% Clorox for 10 min resulted in 85% decontamination and 80% germination. Among different media used to evaluate the rate of seed germination, seeds that were de-coated and transversally cut at the tip and cultured on basal MS medium resulted in 100% germination. The highest percentage of shoot initiation (87%) was obtained on MS medium containing 1.0 mg/l 6-Benzylaminopurine (BAP). The highest mean shoot number per explant (8.5 ± 0.69) was achieved on MS multiplication medium containing 1.5 mg/l BAP in combination with 0.1 mg/l Indole-3-butyric acid (IBA). The highest mean number of roots per explant (3.73 ± 0.69) was obtained on MS medium containing 2.0 mg/l Indole-3-acetic-acid (IAA). Among plantlets transferred to greenhouse, 60% survived after acclimatization. Conclusions This micropropagation protocol can be used for mass propagation of S. longipedunculata that contributes to its conservation and genetic improvement. |
topic |
Micropropagation Plant growth regulators Securidaca longipedunculata Shoot initiation Shoot multiplication |
url |
https://doi.org/10.1186/s43141-019-0017-0 |
work_keys_str_mv |
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