Detection of Helicobacter pylori vacA, cagA and iceA1 virulence genes associated with gastric diseases in Egyptian patients
Background: Helicobactor pylori (H. pylori) virulence markers would be useful to predict peptic ulcer disease (PUD) or gastric cancer. Aim: In Egypt, since inadequate data are present regarding H. pylori virulence–related genes in different age group patients with gastro-duodenal diseases, it become...
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doaj-7d13a05797374be9ac02e9d1b884eeac2020-11-25T02:12:50ZengSpringerOpenEgyptian Journal of Medical Human Genetics1110-86302017-10-01184365371Detection of Helicobacter pylori vacA, cagA and iceA1 virulence genes associated with gastric diseases in Egyptian patientsAhmed El-Shenawy0Manal Diab1Mohamed Shemis2Maged El-Ghannam3Dalia Salem4Moustafa Abdelnasser5Mohamed Shahin6Mahmoud Abdel-Hady7Effat El-Sherbini8Mohamed Saber9Microbiology Department, Theodor Bilharz Research Institute (TBRI), Giza, EgyptMicrobiology Department, Theodor Bilharz Research Institute (TBRI), Giza, EgyptBiochemistry Department, Theodor Bilharz Research Institute (TBRI), Giza, EgyptGastroentrology and Hepatology Department, Theodor Bilharz Research Institute (TBRI), Giza, EgyptMicrobiology Department, Theodor Bilharz Research Institute (TBRI), Giza, Egypt; Corresponding author at: Theodor Bilharz Research Institute (TBRI), EL-Nile street, Warrak EL-Hadar, Imbaba, Giza, P.O. box 12411, Egypt.Medical Microbiology and Immunology Department, Faculty of Medicine Al-Azhar University, EgyptBiochemistry Department, Theodor Bilharz Research Institute (TBRI), Giza, EgyptMedical Microbiology and Immunology Department, Faculty of Medicine Al-Azhar University, EgyptMicrobiology Department, Theodor Bilharz Research Institute (TBRI), Giza, EgyptBiochemistry Department, Theodor Bilharz Research Institute (TBRI), Giza, EgyptBackground: Helicobactor pylori (H. pylori) virulence markers would be useful to predict peptic ulcer disease (PUD) or gastric cancer. Aim: In Egypt, since inadequate data are present regarding H. pylori virulence–related genes in different age group patients with gastro-duodenal diseases, it becomes crucial to study the clinical status of cagA, vacA and iceA1 genotypes of H. pylori strains recovered from patients with dyspepsia. Subjects and methods: The study included 113 dyspeptic patients who were exposed to upper gastrointestinal endoscopic examination. Four antral biopsies were obtained from each patient for the analysis of H. pylori infection by rapid urease test and detection of 16S rRNA. Results: Sixty (53.1%) patients were confirmed to be infected with H. pylori. Upon endoscopy, gastritis was revealed in 27 patients (45%) and10 patients (16.7%) had PUD. Of the 60 H. pylori strains, 39 (65%) had at least one virulence gene. Six different genotypic forms were recognized; vacA (9/60), iceA1 (1/60), vacA/cagA (7/60), vacA/iceA1 (13/60), vacA/cagA/iceA1 (8/60) only one of cagA/iceA type and we could not detect cagA. The overall vacA, iceA1and cagA genes identified were 61.6%, 38.8%, 26.6% respectively, by PCR-based molecular testing. The vacA gene status was highly significant related to gastritis patient (P ≤ 0.036). The vacA s1m1 and s2m2 alleles were significantly found in 50% of H. pylori infected patients with PUD and with gastritis 57.1% respectively (P ≤ 0.01). Conclusion: In conclusion, the main genotype combinations in the studied Egyptian patients were; vacAs2m2/iceA1, vacAs1m1/cagA, mostly associated with gastritis, and vacAs1/cagA/icA, mainly in PUD. The less virulent (s2, s2m2) H. pylori genotypes were found in patients aged over 43 years. Keywords: Helicobactor pylori, vacA, cagA, icA1, Peptic ulcer disease, PCR–based genotyping, Gastric biopsieshttp://www.sciencedirect.com/science/article/pii/S1110863017300435 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ahmed El-Shenawy Manal Diab Mohamed Shemis Maged El-Ghannam Dalia Salem Moustafa Abdelnasser Mohamed Shahin Mahmoud Abdel-Hady Effat El-Sherbini Mohamed Saber |
spellingShingle |
Ahmed El-Shenawy Manal Diab Mohamed Shemis Maged El-Ghannam Dalia Salem Moustafa Abdelnasser Mohamed Shahin Mahmoud Abdel-Hady Effat El-Sherbini Mohamed Saber Detection of Helicobacter pylori vacA, cagA and iceA1 virulence genes associated with gastric diseases in Egyptian patients Egyptian Journal of Medical Human Genetics |
author_facet |
Ahmed El-Shenawy Manal Diab Mohamed Shemis Maged El-Ghannam Dalia Salem Moustafa Abdelnasser Mohamed Shahin Mahmoud Abdel-Hady Effat El-Sherbini Mohamed Saber |
author_sort |
Ahmed El-Shenawy |
title |
Detection of Helicobacter pylori vacA, cagA and iceA1 virulence genes associated with gastric diseases in Egyptian patients |
title_short |
Detection of Helicobacter pylori vacA, cagA and iceA1 virulence genes associated with gastric diseases in Egyptian patients |
title_full |
Detection of Helicobacter pylori vacA, cagA and iceA1 virulence genes associated with gastric diseases in Egyptian patients |
title_fullStr |
Detection of Helicobacter pylori vacA, cagA and iceA1 virulence genes associated with gastric diseases in Egyptian patients |
title_full_unstemmed |
Detection of Helicobacter pylori vacA, cagA and iceA1 virulence genes associated with gastric diseases in Egyptian patients |
title_sort |
detection of helicobacter pylori vaca, caga and icea1 virulence genes associated with gastric diseases in egyptian patients |
publisher |
SpringerOpen |
series |
Egyptian Journal of Medical Human Genetics |
issn |
1110-8630 |
publishDate |
2017-10-01 |
description |
Background: Helicobactor pylori (H. pylori) virulence markers would be useful to predict peptic ulcer disease (PUD) or gastric cancer. Aim: In Egypt, since inadequate data are present regarding H. pylori virulence–related genes in different age group patients with gastro-duodenal diseases, it becomes crucial to study the clinical status of cagA, vacA and iceA1 genotypes of H. pylori strains recovered from patients with dyspepsia. Subjects and methods: The study included 113 dyspeptic patients who were exposed to upper gastrointestinal endoscopic examination. Four antral biopsies were obtained from each patient for the analysis of H. pylori infection by rapid urease test and detection of 16S rRNA. Results: Sixty (53.1%) patients were confirmed to be infected with H. pylori. Upon endoscopy, gastritis was revealed in 27 patients (45%) and10 patients (16.7%) had PUD. Of the 60 H. pylori strains, 39 (65%) had at least one virulence gene. Six different genotypic forms were recognized; vacA (9/60), iceA1 (1/60), vacA/cagA (7/60), vacA/iceA1 (13/60), vacA/cagA/iceA1 (8/60) only one of cagA/iceA type and we could not detect cagA. The overall vacA, iceA1and cagA genes identified were 61.6%, 38.8%, 26.6% respectively, by PCR-based molecular testing. The vacA gene status was highly significant related to gastritis patient (P ≤ 0.036). The vacA s1m1 and s2m2 alleles were significantly found in 50% of H. pylori infected patients with PUD and with gastritis 57.1% respectively (P ≤ 0.01). Conclusion: In conclusion, the main genotype combinations in the studied Egyptian patients were; vacAs2m2/iceA1, vacAs1m1/cagA, mostly associated with gastritis, and vacAs1/cagA/icA, mainly in PUD. The less virulent (s2, s2m2) H. pylori genotypes were found in patients aged over 43 years. Keywords: Helicobactor pylori, vacA, cagA, icA1, Peptic ulcer disease, PCR–based genotyping, Gastric biopsies |
url |
http://www.sciencedirect.com/science/article/pii/S1110863017300435 |
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