Refinement of a colostrum-deprived pig model for infectious disease research
Well-defined pig models are useful to study the pathogenicity of newly recognized pathogens or strains in pigs and serve as animal models for some human diseases. The conventional pig model, where research pigs are sourced from commercial high-health production systems, is commonly used due to the e...
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doaj-7d17646eb9e14e53a9d98d2d1be6a7fa2020-11-25T02:05:29ZengElsevierMethodsX2215-01612018-01-015403413Refinement of a colostrum-deprived pig model for infectious disease researchTanja Opriessnig0Priscilla F. Gerber1Patrick G. Halbur2The Roslin Institute and The Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, Scotland, UK; Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA; Corresponding author at: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.Animal Science, School of Environmental and Rural Science, University of New England, Armidale, New South Wales, AustraliaDepartment of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USAWell-defined pig models are useful to study the pathogenicity of newly recognized pathogens or strains in pigs and serve as animal models for some human diseases. The conventional pig model, where research pigs are sourced from commercial high-health production systems, is commonly used due to the easiness of getting pigs in a timely manner. However, freedom of the pig for the pathogen of interest is important at study start and serological assays to screen pigs for antibodies against newly identified pathogens or molecular assays detecting all possible circulating pathogen variants may not yet exist. Using colostrum-deprived (CD) pigs is a good alternative strategy to circumvent passively-acquired immunity against the pathogen of interest or exposure to pathogens shortly after birth. However, CD pigs are difficult to rear as they are highly susceptible to infections, and mortality rates in the first few days of life are often very high. Herein we report on refinement of a CD pig model with consistent survival rates of 90–100% of the piglets. • Step-by-step protocol to derive and rear CD piglets with higher expected survival rates. • Pig housing improvement minimizes the risk of disease transmission. • Infectious virus disease research pig model purpose. Method name: Colostrum-deprived pig model, Keywords: Pig model, Infectious pathogens, Colostrum-deprived pigs, Naturally-farrowed, Artificially-rearedhttp://www.sciencedirect.com/science/article/pii/S2215016118300505 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tanja Opriessnig Priscilla F. Gerber Patrick G. Halbur |
spellingShingle |
Tanja Opriessnig Priscilla F. Gerber Patrick G. Halbur Refinement of a colostrum-deprived pig model for infectious disease research MethodsX |
author_facet |
Tanja Opriessnig Priscilla F. Gerber Patrick G. Halbur |
author_sort |
Tanja Opriessnig |
title |
Refinement of a colostrum-deprived pig model for infectious disease research |
title_short |
Refinement of a colostrum-deprived pig model for infectious disease research |
title_full |
Refinement of a colostrum-deprived pig model for infectious disease research |
title_fullStr |
Refinement of a colostrum-deprived pig model for infectious disease research |
title_full_unstemmed |
Refinement of a colostrum-deprived pig model for infectious disease research |
title_sort |
refinement of a colostrum-deprived pig model for infectious disease research |
publisher |
Elsevier |
series |
MethodsX |
issn |
2215-0161 |
publishDate |
2018-01-01 |
description |
Well-defined pig models are useful to study the pathogenicity of newly recognized pathogens or strains in pigs and serve as animal models for some human diseases. The conventional pig model, where research pigs are sourced from commercial high-health production systems, is commonly used due to the easiness of getting pigs in a timely manner. However, freedom of the pig for the pathogen of interest is important at study start and serological assays to screen pigs for antibodies against newly identified pathogens or molecular assays detecting all possible circulating pathogen variants may not yet exist. Using colostrum-deprived (CD) pigs is a good alternative strategy to circumvent passively-acquired immunity against the pathogen of interest or exposure to pathogens shortly after birth. However, CD pigs are difficult to rear as they are highly susceptible to infections, and mortality rates in the first few days of life are often very high. Herein we report on refinement of a CD pig model with consistent survival rates of 90–100% of the piglets. • Step-by-step protocol to derive and rear CD piglets with higher expected survival rates. • Pig housing improvement minimizes the risk of disease transmission. • Infectious virus disease research pig model purpose. Method name: Colostrum-deprived pig model, Keywords: Pig model, Infectious pathogens, Colostrum-deprived pigs, Naturally-farrowed, Artificially-reared |
url |
http://www.sciencedirect.com/science/article/pii/S2215016118300505 |
work_keys_str_mv |
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