Protein-species quantitative venomics: looking through a crystal ball
Abstract In this paper we discuss recent significant developments in the field of venom research, specifically the emergence of top-down proteomic applications that allow achieving compositional resolution at the level of the protein species present in the venom, and the absolute quantification of t...
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doaj-7d1c043f647b42c9aeef5d749bd739282020-11-25T01:48:51ZengSciELOJournal of Venomous Animals and Toxins including Tropical Diseases1678-91992017-04-012311910.1186/s40409-017-0116-9Protein-species quantitative venomics: looking through a crystal ballJuan J. Calvete0Daniel Petras1Francisco Calderón-Celis2Bruno Lomonte3Jorge Ruiz Encinar4Alfredo Sanz-Medel5Structural and Functional Venomics Laboratory, Instituto de Biomedicina de Valencia, C.S.I.CSkaggs School of Pharmacy & Pharmaceutical Sciences, University of California—San DiegoDepartment of Physical and Analytical Chemistry, University of OviedoInstituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa RicaDepartment of Physical and Analytical Chemistry, University of OviedoDepartment of Physical and Analytical Chemistry, University of OviedoAbstract In this paper we discuss recent significant developments in the field of venom research, specifically the emergence of top-down proteomic applications that allow achieving compositional resolution at the level of the protein species present in the venom, and the absolute quantification of the venom proteins (the term “protein species” is used here to refer to all the different molecular forms in which a protein can be found. Please consult the special issue of Jornal of Proteomics “Towards deciphering proteomes via the proteoform, protein speciation, moonlighting and protein code concepts” published in 2016, vol. 134, pages 1-202). Challenges remain to be solved in order to achieve a compact and automated platform with which to routinely carry out comprehensive quantitative analysis of all toxins present in a venom. This short essay reflects the authors’ view of the immediate future in this direction for the proteomic analysis of venoms, particularly of snakes.http://link.springer.com/article/10.1186/s40409-017-0116-9Snake venomicsTop-down proteomicsTop-down venomicsProtein species-resolved venomicsAbsolute quantificationInductively coupled plasma mass spectrometry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Juan J. Calvete Daniel Petras Francisco Calderón-Celis Bruno Lomonte Jorge Ruiz Encinar Alfredo Sanz-Medel |
spellingShingle |
Juan J. Calvete Daniel Petras Francisco Calderón-Celis Bruno Lomonte Jorge Ruiz Encinar Alfredo Sanz-Medel Protein-species quantitative venomics: looking through a crystal ball Journal of Venomous Animals and Toxins including Tropical Diseases Snake venomics Top-down proteomics Top-down venomics Protein species-resolved venomics Absolute quantification Inductively coupled plasma mass spectrometry |
author_facet |
Juan J. Calvete Daniel Petras Francisco Calderón-Celis Bruno Lomonte Jorge Ruiz Encinar Alfredo Sanz-Medel |
author_sort |
Juan J. Calvete |
title |
Protein-species quantitative venomics: looking through a crystal ball |
title_short |
Protein-species quantitative venomics: looking through a crystal ball |
title_full |
Protein-species quantitative venomics: looking through a crystal ball |
title_fullStr |
Protein-species quantitative venomics: looking through a crystal ball |
title_full_unstemmed |
Protein-species quantitative venomics: looking through a crystal ball |
title_sort |
protein-species quantitative venomics: looking through a crystal ball |
publisher |
SciELO |
series |
Journal of Venomous Animals and Toxins including Tropical Diseases |
issn |
1678-9199 |
publishDate |
2017-04-01 |
description |
Abstract In this paper we discuss recent significant developments in the field of venom research, specifically the emergence of top-down proteomic applications that allow achieving compositional resolution at the level of the protein species present in the venom, and the absolute quantification of the venom proteins (the term “protein species” is used here to refer to all the different molecular forms in which a protein can be found. Please consult the special issue of Jornal of Proteomics “Towards deciphering proteomes via the proteoform, protein speciation, moonlighting and protein code concepts” published in 2016, vol. 134, pages 1-202). Challenges remain to be solved in order to achieve a compact and automated platform with which to routinely carry out comprehensive quantitative analysis of all toxins present in a venom. This short essay reflects the authors’ view of the immediate future in this direction for the proteomic analysis of venoms, particularly of snakes. |
topic |
Snake venomics Top-down proteomics Top-down venomics Protein species-resolved venomics Absolute quantification Inductively coupled plasma mass spectrometry |
url |
http://link.springer.com/article/10.1186/s40409-017-0116-9 |
work_keys_str_mv |
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