Antibacterial Activity of Commercial Dentine Bonding Systems against E. faecalis–Flow Cytometry Study

• Main Authors: Monika Lukomska-Szymanska, Magdalena Konieczka, Beata Zarzycka, Barbara Lapinska, Janina Grzegorczyk, Jerzy Sokolowski
• Format: Article
• Language: English
• Published: MDPI AG 2017-04-01
• Series: Materials
• Subjects: dental bonding systems; flow cytometry; E. faecalis; antibacterial activity
• Online Access: http://www.mdpi.com/1996-1944/10/5/481

Literature presents inconsistent results on the antibacterial activity of dentine bonding systems (DBS). Antibacterial activity of adhesive systems depends on several factors, including composition and acidity. Flow cytometry is a novel detection method to measure multiple characteristics of a single cell: total cell number, structural (size, shape), and functional parameters (viability, cell cycle). The LIVE/DEAD® BacLightTM bacterial viability assay was used to evaluate an antibacterial activity of DBS by assessing physical membrane disruption of bacteria mediated by DBS. Ten commercial DBSs: four total-etching (TE), four self-etching (SE) and two selective enamel etching (SEE) were tested. Both total-etching DBS ExciTE F and OptiBond Solo Plus showed comparatively low antibacterial activity against E. faecalis. The lowest activity of all tested TE systems showed Te-Econom Bond. Among SE DBS, G-ænial Bond (92.24% dead cells) followed by Clearfil S3 Bond Plus (88.02%) and Panavia F 2.0 ED Primer II (86.67%) showed the highest antibacterial activity against E. faecalis, which was comparable to isopropranol (positive control). In the present study, self-etching DBS exhibited higher antimicrobial activity than tested total-etching adhesives against E. faecalis.