Longitudinal study of changes in γδ T cells and CD4+ T cells upon asymptomatic malaria infection in Indonesian children
Abstract Both γδ T cells and CD4+ T cells have been implicated in immunity to malaria, but their association with natural gain or loss of infection has not been studied before. Therefore, we followed up asymptomatic children living in an area endemic for malaria in Indonesia for 21 months. The perce...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2017-08-01
|
Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-017-09099-z |
id |
doaj-7d52068d2c9941339627fa2d9bd14787 |
---|---|
record_format |
Article |
spelling |
doaj-7d52068d2c9941339627fa2d9bd147872020-12-08T03:11:16ZengNature Publishing GroupScientific Reports2045-23222017-08-01711710.1038/s41598-017-09099-zLongitudinal study of changes in γδ T cells and CD4+ T cells upon asymptomatic malaria infection in Indonesian childrenSanne E. de Jong0Vera E. R. Asscher1Linda J. Wammes2Aprilianto E. Wiria3Firdaus Hamid4Erliyani Sartono5Taniawati Supali6Hermelijn H. Smits7Adrian J. F. Luty8Maria Yazdanbakhsh9Leiden Immunoparasitology Group, Department of Parasitology, Leiden University Medical CenterLeiden Immunoparasitology Group, Department of Parasitology, Leiden University Medical CenterLeiden Immunoparasitology Group, Department of Parasitology, Leiden University Medical CenterLeiden Immunoparasitology Group, Department of Parasitology, Leiden University Medical CenterLeiden Immunoparasitology Group, Department of Parasitology, Leiden University Medical CenterLeiden Immunoparasitology Group, Department of Parasitology, Leiden University Medical CenterDepartment of Parasitology, University of IndonesiaLeiden Immunoparasitology Group, Department of Parasitology, Leiden University Medical CenterMère et Enfant Face aux Infections Tropicales, UMR 216, French National Research Institute for Sustainable Development (IRD)Leiden Immunoparasitology Group, Department of Parasitology, Leiden University Medical CenterAbstract Both γδ T cells and CD4+ T cells have been implicated in immunity to malaria, but their association with natural gain or loss of infection has not been studied before. Therefore, we followed up asymptomatic children living in an area endemic for malaria in Indonesia for 21 months. The percentage of γδ T cells was related to both current and previous infection, with higher percentages in infected than uninfected children and declining after infections resolve. Infected children also had higher levels of Th1 and Th17 cells, lower levels of CD25Hi FOXP3+ regulatory T cells (Tregs), but similar levels of Th2 cells as compared to uninfected children. However, TNF, IFN-γ, and IL-17 cytokine responses to Plasmodium falciparum-infected red blood cells (PfRBCs) were similar, while IL-5 and IL-13 responses were lower in infected children. Furthermore, infected children had more phenotypically exhausted PD-1+ CD4+ T cells, more Tregs expressing TNF-RII, and higher IL-10 responses to PfRBCs, which persisted following resolution of infection. Altogether, this study demonstrates that asymptomatic malaria infection is associated with some long-lasting changes in the frequencies and immunoregulation of circulating innate and adaptive T cells, which might in part explain how pre-exposure to malaria affects responses to subsequent immunological challenges.https://doi.org/10.1038/s41598-017-09099-z |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sanne E. de Jong Vera E. R. Asscher Linda J. Wammes Aprilianto E. Wiria Firdaus Hamid Erliyani Sartono Taniawati Supali Hermelijn H. Smits Adrian J. F. Luty Maria Yazdanbakhsh |
spellingShingle |
Sanne E. de Jong Vera E. R. Asscher Linda J. Wammes Aprilianto E. Wiria Firdaus Hamid Erliyani Sartono Taniawati Supali Hermelijn H. Smits Adrian J. F. Luty Maria Yazdanbakhsh Longitudinal study of changes in γδ T cells and CD4+ T cells upon asymptomatic malaria infection in Indonesian children Scientific Reports |
author_facet |
Sanne E. de Jong Vera E. R. Asscher Linda J. Wammes Aprilianto E. Wiria Firdaus Hamid Erliyani Sartono Taniawati Supali Hermelijn H. Smits Adrian J. F. Luty Maria Yazdanbakhsh |
author_sort |
Sanne E. de Jong |
title |
Longitudinal study of changes in γδ T cells and CD4+ T cells upon asymptomatic malaria infection in Indonesian children |
title_short |
Longitudinal study of changes in γδ T cells and CD4+ T cells upon asymptomatic malaria infection in Indonesian children |
title_full |
Longitudinal study of changes in γδ T cells and CD4+ T cells upon asymptomatic malaria infection in Indonesian children |
title_fullStr |
Longitudinal study of changes in γδ T cells and CD4+ T cells upon asymptomatic malaria infection in Indonesian children |
title_full_unstemmed |
Longitudinal study of changes in γδ T cells and CD4+ T cells upon asymptomatic malaria infection in Indonesian children |
title_sort |
longitudinal study of changes in γδ t cells and cd4+ t cells upon asymptomatic malaria infection in indonesian children |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-08-01 |
description |
Abstract Both γδ T cells and CD4+ T cells have been implicated in immunity to malaria, but their association with natural gain or loss of infection has not been studied before. Therefore, we followed up asymptomatic children living in an area endemic for malaria in Indonesia for 21 months. The percentage of γδ T cells was related to both current and previous infection, with higher percentages in infected than uninfected children and declining after infections resolve. Infected children also had higher levels of Th1 and Th17 cells, lower levels of CD25Hi FOXP3+ regulatory T cells (Tregs), but similar levels of Th2 cells as compared to uninfected children. However, TNF, IFN-γ, and IL-17 cytokine responses to Plasmodium falciparum-infected red blood cells (PfRBCs) were similar, while IL-5 and IL-13 responses were lower in infected children. Furthermore, infected children had more phenotypically exhausted PD-1+ CD4+ T cells, more Tregs expressing TNF-RII, and higher IL-10 responses to PfRBCs, which persisted following resolution of infection. Altogether, this study demonstrates that asymptomatic malaria infection is associated with some long-lasting changes in the frequencies and immunoregulation of circulating innate and adaptive T cells, which might in part explain how pre-exposure to malaria affects responses to subsequent immunological challenges. |
url |
https://doi.org/10.1038/s41598-017-09099-z |
work_keys_str_mv |
AT sanneedejong longitudinalstudyofchangesingdtcellsandcd4tcellsuponasymptomaticmalariainfectioninindonesianchildren AT veraerasscher longitudinalstudyofchangesingdtcellsandcd4tcellsuponasymptomaticmalariainfectioninindonesianchildren AT lindajwammes longitudinalstudyofchangesingdtcellsandcd4tcellsuponasymptomaticmalariainfectioninindonesianchildren AT apriliantoewiria longitudinalstudyofchangesingdtcellsandcd4tcellsuponasymptomaticmalariainfectioninindonesianchildren AT firdaushamid longitudinalstudyofchangesingdtcellsandcd4tcellsuponasymptomaticmalariainfectioninindonesianchildren AT erliyanisartono longitudinalstudyofchangesingdtcellsandcd4tcellsuponasymptomaticmalariainfectioninindonesianchildren AT taniawatisupali longitudinalstudyofchangesingdtcellsandcd4tcellsuponasymptomaticmalariainfectioninindonesianchildren AT hermelijnhsmits longitudinalstudyofchangesingdtcellsandcd4tcellsuponasymptomaticmalariainfectioninindonesianchildren AT adrianjfluty longitudinalstudyofchangesingdtcellsandcd4tcellsuponasymptomaticmalariainfectioninindonesianchildren AT mariayazdanbakhsh longitudinalstudyofchangesingdtcellsandcd4tcellsuponasymptomaticmalariainfectioninindonesianchildren |
_version_ |
1724392829892952064 |