Evaluation of Lipid metabolizing enzymes: Paraxonase1(PON1) and Lecithin Cholesterol acyltransferase (LCAT) activities in children with nephrotic syndrome
Background: The most common glomerular disorder in children is nephrotic syndrome, associated with high morbidity despite notable advances in its treatment. Many of the nephrotic syndrome complications, including the increased risk of atherosclerosis and thromboembolism, can be linked to dysregulat...
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College of Medicine, Al-Nahrain University
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doaj-7d6a8b1d3dfe4379ba369a32c3c068fd2021-08-31T11:25:44ZengCollege of Medicine, Al-Nahrain UniversityBaghdad Journal of Biochemistry and Applied Biological Sciences2706-99152021-05-01201Evaluation of Lipid metabolizing enzymes: Paraxonase1(PON1) and Lecithin Cholesterol acyltransferase (LCAT) activities in children with nephrotic syndromeRaghad Jamal Ali 0Rayah Sulaiman Baban 1Shatha Hussain Ali 2Ministry of Health, Baghdad Medical City, Baghdad, IraqDepartment of Chemistry and Biochemistry, College of Medicine, Al- Nahrain University, Baghdad, IraqDepartment of Pediatrics, College of Medicine, Al- Nahrain University, Baghdad, Iraq Background: The most common glomerular disorder in children is nephrotic syndrome, associated with high morbidity despite notable advances in its treatment. Many of the nephrotic syndrome complications, including the increased risk of atherosclerosis and thromboembolism, can be linked to dysregulated lipid metabolism and dyslipidemia. Paraoxonase enzyme is responsible for the most of antioxidant properties of HDL, thus preventing the formation of atherogenic ox-LDL molecules, and Lecithin Cholesterol acyltransferase is intimately involved in HDL maturation and is a key component of the reverse cholesterol transport pathway, which removes excess cholesterol molecules from the peripheral tissues to the liver for excretion. Objective: The present study aimed to investigate the serum activities of paraoxonase-1(PON-1) and lecithin cholesterol acyltransferase (LCAT) in children with nephrotic syndrome in an active phase (as newly diagnosed or old cases with acute relapse) also, to study any correlation exists between paraoxonase-1 activity and lipid profile. Methods: This study consists of Group 1 with 40 cases of nephrotic syndrome in the age group of (2-14 years) and Group 2 with 40 age and sex-matched healthy controls. Lipid profile and paraoxonase activity, Lecithin Cholesterol acyltransferase activities were measured in both groups' serum samples. Results: Statistical analysis by student's t-test showed that the mean levels of Total Cholesterol, Triglycerides, LDL were significantly increased in Group 1 when compared to Group 2 (p <0.001). PON1 and Lecithin Cholesterol acyltransferase levels were significantly lowered in Group 1 compared to Group 2, and there is no significant difference among nephrotic groups. Conclusion: Both Paraoxonase-1 enzyme and Lecithin Cholesterol acyltransferase are considered good promising predictors for nephrotic syndrome and other parameters such as LDL, HDL, and T.G. The significantly decreased Paraoxonase-1 enzyme and Lecithin Cholesterol acyltransferase activities result in increased oxidation of LDL, thus accelerating atherosclerosis. https://bjbabs.org/index.php/bjbabs/article/view/38Lipid metabolizing enzymesParaxonase1PON1Lecithin Cholesterol acyltransferaseLCATNephrotic Syndrome |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Raghad Jamal Ali Rayah Sulaiman Baban Shatha Hussain Ali |
spellingShingle |
Raghad Jamal Ali Rayah Sulaiman Baban Shatha Hussain Ali Evaluation of Lipid metabolizing enzymes: Paraxonase1(PON1) and Lecithin Cholesterol acyltransferase (LCAT) activities in children with nephrotic syndrome Baghdad Journal of Biochemistry and Applied Biological Sciences Lipid metabolizing enzymes Paraxonase1 PON1 Lecithin Cholesterol acyltransferase LCAT Nephrotic Syndrome |
author_facet |
Raghad Jamal Ali Rayah Sulaiman Baban Shatha Hussain Ali |
author_sort |
Raghad Jamal Ali |
title |
Evaluation of Lipid metabolizing enzymes: Paraxonase1(PON1) and Lecithin Cholesterol acyltransferase (LCAT) activities in children with nephrotic syndrome |
title_short |
Evaluation of Lipid metabolizing enzymes: Paraxonase1(PON1) and Lecithin Cholesterol acyltransferase (LCAT) activities in children with nephrotic syndrome |
title_full |
Evaluation of Lipid metabolizing enzymes: Paraxonase1(PON1) and Lecithin Cholesterol acyltransferase (LCAT) activities in children with nephrotic syndrome |
title_fullStr |
Evaluation of Lipid metabolizing enzymes: Paraxonase1(PON1) and Lecithin Cholesterol acyltransferase (LCAT) activities in children with nephrotic syndrome |
title_full_unstemmed |
Evaluation of Lipid metabolizing enzymes: Paraxonase1(PON1) and Lecithin Cholesterol acyltransferase (LCAT) activities in children with nephrotic syndrome |
title_sort |
evaluation of lipid metabolizing enzymes: paraxonase1(pon1) and lecithin cholesterol acyltransferase (lcat) activities in children with nephrotic syndrome |
publisher |
College of Medicine, Al-Nahrain University |
series |
Baghdad Journal of Biochemistry and Applied Biological Sciences |
issn |
2706-9915 |
publishDate |
2021-05-01 |
description |
Background: The most common glomerular disorder in children is nephrotic syndrome, associated with high morbidity despite notable advances in its treatment. Many of the nephrotic syndrome complications, including the increased risk of atherosclerosis and thromboembolism, can be linked to dysregulated lipid metabolism and dyslipidemia. Paraoxonase enzyme is responsible for the most of antioxidant properties of HDL, thus preventing the formation of atherogenic ox-LDL molecules, and Lecithin Cholesterol acyltransferase is intimately involved in HDL maturation and is a key component of the reverse cholesterol transport pathway, which removes excess cholesterol molecules from the peripheral tissues to the liver for excretion.
Objective: The present study aimed to investigate the serum activities of paraoxonase-1(PON-1) and lecithin cholesterol acyltransferase (LCAT) in children with nephrotic syndrome in an active phase (as newly diagnosed or old cases with acute relapse)
also, to study any correlation exists between paraoxonase-1 activity and lipid profile.
Methods: This study consists of Group 1 with 40 cases of nephrotic syndrome in the age group of (2-14 years) and Group 2 with 40 age and sex-matched healthy controls. Lipid profile and paraoxonase activity, Lecithin Cholesterol acyltransferase activities were measured in both groups' serum samples.
Results: Statistical analysis by student's t-test showed that the mean levels of Total Cholesterol, Triglycerides, LDL were significantly increased in Group 1 when compared to Group 2 (p <0.001). PON1 and Lecithin Cholesterol acyltransferase levels were significantly lowered in Group 1 compared to Group 2, and there is no significant difference among nephrotic groups.
Conclusion: Both Paraoxonase-1 enzyme and Lecithin Cholesterol acyltransferase are considered good promising predictors for nephrotic syndrome and other parameters such as LDL, HDL, and T.G. The significantly decreased Paraoxonase-1 enzyme and Lecithin Cholesterol acyltransferase activities result in increased oxidation of LDL, thus accelerating atherosclerosis.
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topic |
Lipid metabolizing enzymes Paraxonase1 PON1 Lecithin Cholesterol acyltransferase LCAT Nephrotic Syndrome |
url |
https://bjbabs.org/index.php/bjbabs/article/view/38 |
work_keys_str_mv |
AT raghadjamalali evaluationoflipidmetabolizingenzymesparaxonase1pon1andlecithincholesterolacyltransferaselcatactivitiesinchildrenwithnephroticsyndrome AT rayahsulaimanbaban evaluationoflipidmetabolizingenzymesparaxonase1pon1andlecithincholesterolacyltransferaselcatactivitiesinchildrenwithnephroticsyndrome AT shathahussainali evaluationoflipidmetabolizingenzymesparaxonase1pon1andlecithincholesterolacyltransferaselcatactivitiesinchildrenwithnephroticsyndrome |
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