GoPrime: Development of an In Silico Framework to Predict the Performance of Real-Time PCR Primers and Probes Using Foot-and-Mouth Disease Virus as a Model
Real-time PCR (rPCR) is a widely accepted diagnostic tool for the detection and quantification of nucleic acid targets. In order for these assays to achieve high sensitivity and specificity, primer and probe-template complementarity is essential; however, mismatches are often unavoidable and can res...
Main Authors: | Emma L A Howson, Richard J Orton, Valerie Mioulet, Tiziana Lembo, Donald P King, Veronica L Fowler |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-04-01
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Series: | Pathogens |
Subjects: | |
Online Access: | https://www.mdpi.com/2076-0817/9/4/303 |
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