Evaluation of the Effect of Nigella Sativa Hydro-alcoholic Extract and Honey on Gonadotropins and Sex Hormones Level in the Polycystic Ovarian Syndrome Model of Wistar Rat
Background and Aim: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders of women in reproductive age. Anovulation and hyperandrogenism due to PCOS are major causes of infertility. The aim of the present study was to evaluate the combined effect of Nigella Sativa hydro-alco...
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Kurdistan University of Medical Sciences
2020-03-01
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doaj-7d6f6e3536324a319f8ba759c74031af2020-12-22T07:28:11Zfas Kurdistan University of Medical Sciencesمجله علمی دانشگاه علوم پزشکی کردستان1560-652X2345-40402020-03-01251117129Evaluation of the Effect of Nigella Sativa Hydro-alcoholic Extract and Honey on Gonadotropins and Sex Hormones Level in the Polycystic Ovarian Syndrome Model of Wistar RatSeiyedeh Narjes Naseran0Mokhtar Mokhtari1Mahmood Abedinzade2Mehrdad Shariati3 Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran Department of Physiology, Medical Biotechnology Research Center, School of Paramedicine, Guilan University of Medical Sciences, Rasht, Iran Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran Background and Aim: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders of women in reproductive age. Anovulation and hyperandrogenism due to PCOS are major causes of infertility. The aim of the present study was to evaluate the combined effect of Nigella Sativa hydro-alcoholic extract and honey on gonadotropins and sex hormones level inpolycystic ovary syndrome in rats. Materials and Methods: 40 adult Wistar rats, weighting 200 -220 g were divided randomly into 5 groups: including intact, Sham (letrozole solvent), PCOS and 2 experimental PCOS groups. Rats were treated with letrozole for 21 days to induce PCOS. In the experimental groups, PCOS rats were treated with 2 doses of combination 300mg/kg Nigella sativa extract with 1200mg/kg honey and 600mg/kg Nigella sativa extract with 2400mg/kg honey for 28 days. The serum levels of LH, FSH, estrogen, progesterone and testosterone were measured by ELISA method and each serum were analyzed using ANOVA and TUKEY statistical tests (P<0.05). Results: A significant decrease was seen in serum levels of LH, estrogen and testosterone (P<0.05) in PCOS group treated with maximum dose of combination of N. sativa extract and Honey compared with the PCOS group. Whereas, a significant increase in serum levels FSH in PCOS group treated with maximum dose and progesterone in the PCOS group treated with maximum and minimum dose of combination of N. sativa extract and Honey compared with the PCOS group (p< 0.05). Conclusion: Beneficial effects of combination of N. sativa extract and Honey was seen but further study is needed.http://sjku.muk.ac.ir/article-1-5090-en.htmlnigella sativahoneypolycystic ovarian syndromerat |
collection |
DOAJ |
language |
fas |
format |
Article |
sources |
DOAJ |
author |
Seiyedeh Narjes Naseran Mokhtar Mokhtari Mahmood Abedinzade Mehrdad Shariati |
spellingShingle |
Seiyedeh Narjes Naseran Mokhtar Mokhtari Mahmood Abedinzade Mehrdad Shariati Evaluation of the Effect of Nigella Sativa Hydro-alcoholic Extract and Honey on Gonadotropins and Sex Hormones Level in the Polycystic Ovarian Syndrome Model of Wistar Rat مجله علمی دانشگاه علوم پزشکی کردستان nigella sativa honey polycystic ovarian syndrome rat |
author_facet |
Seiyedeh Narjes Naseran Mokhtar Mokhtari Mahmood Abedinzade Mehrdad Shariati |
author_sort |
Seiyedeh Narjes Naseran |
title |
Evaluation of the Effect of Nigella Sativa Hydro-alcoholic Extract and Honey on Gonadotropins and Sex Hormones Level in the Polycystic Ovarian Syndrome Model of Wistar Rat |
title_short |
Evaluation of the Effect of Nigella Sativa Hydro-alcoholic Extract and Honey on Gonadotropins and Sex Hormones Level in the Polycystic Ovarian Syndrome Model of Wistar Rat |
title_full |
Evaluation of the Effect of Nigella Sativa Hydro-alcoholic Extract and Honey on Gonadotropins and Sex Hormones Level in the Polycystic Ovarian Syndrome Model of Wistar Rat |
title_fullStr |
Evaluation of the Effect of Nigella Sativa Hydro-alcoholic Extract and Honey on Gonadotropins and Sex Hormones Level in the Polycystic Ovarian Syndrome Model of Wistar Rat |
title_full_unstemmed |
Evaluation of the Effect of Nigella Sativa Hydro-alcoholic Extract and Honey on Gonadotropins and Sex Hormones Level in the Polycystic Ovarian Syndrome Model of Wistar Rat |
title_sort |
evaluation of the effect of nigella sativa hydro-alcoholic extract and honey on gonadotropins and sex hormones level in the polycystic ovarian syndrome model of wistar rat |
publisher |
Kurdistan University of Medical Sciences |
series |
مجله علمی دانشگاه علوم پزشکی کردستان |
issn |
1560-652X 2345-4040 |
publishDate |
2020-03-01 |
description |
Background and Aim: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders of women in reproductive age. Anovulation and hyperandrogenism due to PCOS are major causes of infertility. The aim of the present study was to evaluate the combined effect of Nigella Sativa hydro-alcoholic extract and honey on gonadotropins and sex hormones level inpolycystic ovary syndrome in rats.
Materials and Methods: 40 adult Wistar rats, weighting 200 -220 g were divided randomly into 5 groups: including intact, Sham (letrozole solvent), PCOS and 2 experimental PCOS groups. Rats were treated with letrozole for 21 days to induce PCOS. In the experimental groups, PCOS rats were treated with 2 doses of combination 300mg/kg Nigella sativa extract with 1200mg/kg honey and 600mg/kg Nigella sativa extract with 2400mg/kg honey for 28 days. The serum levels of LH, FSH, estrogen, progesterone and testosterone were measured by ELISA method and each serum were analyzed using ANOVA and TUKEY statistical tests (P<0.05).
Results: A significant decrease was seen in serum levels of LH, estrogen and testosterone (P<0.05) in PCOS group treated with maximum dose of combination of N. sativa extract and Honey compared with the PCOS group. Whereas, a significant increase in serum levels FSH in PCOS group treated with maximum dose and progesterone in the PCOS group treated with maximum and minimum dose of combination of N. sativa extract and Honey compared with the PCOS group (p< 0.05).
Conclusion: Beneficial effects of combination of N. sativa extract and Honey was seen but further study is needed. |
topic |
nigella sativa honey polycystic ovarian syndrome rat |
url |
http://sjku.muk.ac.ir/article-1-5090-en.html |
work_keys_str_mv |
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