Determination of L-Ascorbic Acid Using MBs-AOX/GO/IGZO/Al by Wireless Sensing System and Microfluidic Framework

• Main Authors: Jung-Chuan Chou, You-Xiang Wu, Po-Yu Kuo, Chih-Hsien Lai, Yu-Hsun Nien, Si-Hong Lin, Siao-Jie Yan, Cian-Yi Wu
• Format: Article
• Language: English
• Published: IEEE 2019-01-01
• Series: IEEE Access
• Subjects: L-ascorbic acid (L-AA); graphene oxide (GO); magnetic beads (MBs); microfluidic framework; wireless sensing system
• Online Access: https://ieeexplore.ieee.org/document/8675910/
• ISSN: 2169-3536

In this paper, the L-ascorbic acid (L-AA) biosensor based on magnetic beads-ascorbate oxidase/graphene oxide/indium gallium zinc oxide/aluminum (MBs-AOX/GO/IGZO/Al) membrane was integrated with the potentiometric measurement system with the microfluidic framework in order to investigate the different sensing characteristics under the static condition and the dynamic condition. The L-AA biosensor showed the average sensitivity of 78.9 mV/decade (25 °C) under the static condition, and it showed the average sensitivity of 81.7 mV/decade (25 °C) at the optimal flow rate (25 μl/min). Besides, the XBee module was used to apply in the remote detection for the L-AA biosensor, and the experimental results were shown the average sensitivity of 78.7 mV/decade (25 °C). Moreover, we also investigated various experiments, such as temperature effect, hysteresis effect, and lifetime. These experimental results would be beneficial to the development of the L-AA biosensor based on electrochemical detection.