Validation of the Turkey Semen Cryopreservation by Evaluating the Effect of Two Diluents and the Inseminating Doses
This study was designed to test the fertilizing ability of cryopreserved turkey semen, and here, two experiments were performed: an in vitro analysis to assess the effects of Tselutin and Lake diluents and an in vivo test to determine the fertility and hatching rates by also studying the feat of thr...
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doaj-7d7dbc2d507a427786d55cfe09c348262020-11-25T03:14:20ZengMDPI AGAnimals2076-26152020-08-01101329132910.3390/ani10081329Validation of the Turkey Semen Cryopreservation by Evaluating the Effect of Two Diluents and the Inseminating DosesMichele Di Iorio0Giusy Rusco1Roberta Iampietro2Lucia Maiuro3Achille Schiavone4Silvia Cerolini5Nicolaia Iaffaldano6Department of Agricultural, Environmental and Food Sciences, University of Molise, 86100 Campobasso CB, ItalyDepartment of Agricultural, Environmental and Food Sciences, University of Molise, 86100 Campobasso CB, ItalyDepartment of Agricultural, Environmental and Food Sciences, University of Molise, 86100 Campobasso CB, ItalyDepartment of Agricultural, Environmental and Food Sciences, University of Molise, 86100 Campobasso CB, ItalyDepartment of Veterinary Science, University of Turin, 10095 Torino TO, ItalyDepartment of Veterinary Medicine, University of Milan, 20122 Milano MI, ItalyDepartment of Agricultural, Environmental and Food Sciences, University of Molise, 86100 Campobasso CB, ItalyThis study was designed to test the fertilizing ability of cryopreserved turkey semen, and here, two experiments were performed: an in vitro analysis to assess the effects of Tselutin and Lake diluents and an in vivo test to determine the fertility and hatching rates by also studying the feat of three insemination doses (250, 400 and 600 × 10<sup>6</sup> sperm/hen). Pooled semen samples were diluted with Tselutin or Lake extender which contained 20% of dimethylsulfoxide and 1 mM of Ficoll at final sperm concentration of 3 × 10<sup>9</sup> sperm/mL. Thereafter, semen was packaged into straws and frozen on liquid nitrogen. The post-thaw sperm quality was evaluated considering motility (computer-aided sperm analysis—CASA system) and membrane integrity (flow cytometry). Significantly higher values of progressive motility and some kinetic parameters in semen frozen with Lake were found. When we compared the extenders in vivo, no significant effects were detected, whilst sperm concentration significantly affected both fertility and hatching rates, with the best results obtained with the sperm concentration of 400 × 10<sup>6</sup> sperm/hen. From the results obtained, it emerged that the extender type only affected sperm motility characteristics, not the fertilizing ability of frozen-thawed semen, while inseminating dose markedly affected fertility and hatching rates.https://www.mdpi.com/2076-2615/10/8/1329turkey semenextenderinseminating dosefertilityhatching rate |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Michele Di Iorio Giusy Rusco Roberta Iampietro Lucia Maiuro Achille Schiavone Silvia Cerolini Nicolaia Iaffaldano |
spellingShingle |
Michele Di Iorio Giusy Rusco Roberta Iampietro Lucia Maiuro Achille Schiavone Silvia Cerolini Nicolaia Iaffaldano Validation of the Turkey Semen Cryopreservation by Evaluating the Effect of Two Diluents and the Inseminating Doses Animals turkey semen extender inseminating dose fertility hatching rate |
author_facet |
Michele Di Iorio Giusy Rusco Roberta Iampietro Lucia Maiuro Achille Schiavone Silvia Cerolini Nicolaia Iaffaldano |
author_sort |
Michele Di Iorio |
title |
Validation of the Turkey Semen Cryopreservation by Evaluating the Effect of Two Diluents and the Inseminating Doses |
title_short |
Validation of the Turkey Semen Cryopreservation by Evaluating the Effect of Two Diluents and the Inseminating Doses |
title_full |
Validation of the Turkey Semen Cryopreservation by Evaluating the Effect of Two Diluents and the Inseminating Doses |
title_fullStr |
Validation of the Turkey Semen Cryopreservation by Evaluating the Effect of Two Diluents and the Inseminating Doses |
title_full_unstemmed |
Validation of the Turkey Semen Cryopreservation by Evaluating the Effect of Two Diluents and the Inseminating Doses |
title_sort |
validation of the turkey semen cryopreservation by evaluating the effect of two diluents and the inseminating doses |
publisher |
MDPI AG |
series |
Animals |
issn |
2076-2615 |
publishDate |
2020-08-01 |
description |
This study was designed to test the fertilizing ability of cryopreserved turkey semen, and here, two experiments were performed: an in vitro analysis to assess the effects of Tselutin and Lake diluents and an in vivo test to determine the fertility and hatching rates by also studying the feat of three insemination doses (250, 400 and 600 × 10<sup>6</sup> sperm/hen). Pooled semen samples were diluted with Tselutin or Lake extender which contained 20% of dimethylsulfoxide and 1 mM of Ficoll at final sperm concentration of 3 × 10<sup>9</sup> sperm/mL. Thereafter, semen was packaged into straws and frozen on liquid nitrogen. The post-thaw sperm quality was evaluated considering motility (computer-aided sperm analysis—CASA system) and membrane integrity (flow cytometry). Significantly higher values of progressive motility and some kinetic parameters in semen frozen with Lake were found. When we compared the extenders in vivo, no significant effects were detected, whilst sperm concentration significantly affected both fertility and hatching rates, with the best results obtained with the sperm concentration of 400 × 10<sup>6</sup> sperm/hen. From the results obtained, it emerged that the extender type only affected sperm motility characteristics, not the fertilizing ability of frozen-thawed semen, while inseminating dose markedly affected fertility and hatching rates. |
topic |
turkey semen extender inseminating dose fertility hatching rate |
url |
https://www.mdpi.com/2076-2615/10/8/1329 |
work_keys_str_mv |
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