PCR Detection of Mimivirus

In an epidemiological study, mimivirus was reported as an unlikely cause of human respiratory infections in China. Our analysis revealed the nonsensitivity of the PCR method, which detected less than 10% of the current known mimivirus. We conclude that epidemiologic studies must use accurate and sen...

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Main Authors: Didier Raoult, Anthony Levasseur, Bernard La Scola
Format: Article
Language:English
Published: Centers for Disease Control and Prevention 2017-06-01
Series:Emerging Infectious Diseases
Subjects:
Online Access:https://wwwnc.cdc.gov/eid/article/23/6/16-1896_article
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spelling doaj-7d863f38701e46e899f9a7ff03d197dc2020-11-25T01:37:18ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592017-06-012361044104510.3201/eid2306.161896PCR Detection of MimivirusDidier RaoultAnthony LevasseurBernard La ScolaIn an epidemiological study, mimivirus was reported as an unlikely cause of human respiratory infections in China. Our analysis revealed the nonsensitivity of the PCR method, which detected less than 10% of the current known mimivirus. We conclude that epidemiologic studies must use accurate and sensitive laboratory test methods.https://wwwnc.cdc.gov/eid/article/23/6/16-1896_articlemimiviruspneumoniafalse negative PCRvirusesprimer/probe mismatch
collection DOAJ
language English
format Article
sources DOAJ
author Didier Raoult
Anthony Levasseur
Bernard La Scola
spellingShingle Didier Raoult
Anthony Levasseur
Bernard La Scola
PCR Detection of Mimivirus
Emerging Infectious Diseases
mimivirus
pneumonia
false negative PCR
viruses
primer/probe mismatch
author_facet Didier Raoult
Anthony Levasseur
Bernard La Scola
author_sort Didier Raoult
title PCR Detection of Mimivirus
title_short PCR Detection of Mimivirus
title_full PCR Detection of Mimivirus
title_fullStr PCR Detection of Mimivirus
title_full_unstemmed PCR Detection of Mimivirus
title_sort pcr detection of mimivirus
publisher Centers for Disease Control and Prevention
series Emerging Infectious Diseases
issn 1080-6040
1080-6059
publishDate 2017-06-01
description In an epidemiological study, mimivirus was reported as an unlikely cause of human respiratory infections in China. Our analysis revealed the nonsensitivity of the PCR method, which detected less than 10% of the current known mimivirus. We conclude that epidemiologic studies must use accurate and sensitive laboratory test methods.
topic mimivirus
pneumonia
false negative PCR
viruses
primer/probe mismatch
url https://wwwnc.cdc.gov/eid/article/23/6/16-1896_article
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