Characterization of Ectomycorrhizal species through molecular biology tools and morphotyping
ABSTRACT: Mycorrhizae are mutualistic associations between fungi and plant roots. These symbiotic associations are abundant and occur in 75 to 80 % of plants. Ectomycorrhizal fungi are very important in ecosystems, because their mutualistic association with plants of different species helps nutrient...
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doaj-7d9a26cee49a4d848879d6643303a2d72020-11-25T00:34:41ZengUniversidade de São PauloScientia Agricola1678-992X75324625410.1590/1678-992x-2016-0419S0103-90162018000300246Characterization of Ectomycorrhizal species through molecular biology tools and morphotypingBárbara Estevão ClasenAndressa de Oliveira SilveiraDaiana Bortoluzzi BaldoniDaiane Fiuza MontagnerRodrigo Josemar Seminoti JacquesZaida Inês AntoniolliABSTRACT: Mycorrhizae are mutualistic associations between fungi and plant roots. These symbiotic associations are abundant and occur in 75 to 80 % of plants. Ectomycorrhizal fungi are very important in ecosystems, because their mutualistic association with plants of different species helps nutrients and water absorption, as well as protection of the host plant against pathogens and abiotic stresses. Most ectomycorrhizal fungi belong to the Basidiomycota class, such as the following genera: Amanita, Hebeloma, Hysterangium, Laccaria, Lactarius, Rhizopogon, Russula, Scleroderma, Suillus, Tricholoma, among others. Morphological studies on ectomycorrhizae report important results in understanding the species biodiversity. However, the use of molecular biology nowadays is indispensable. Among the various molecular tools available, there is consensus about the use of tools based on sequencing of the Internal Transcribed Spacer (ITS) of fungi rDNA, aiding in species characterization and construction of phylogenetic studies. The ITS region is of easy amplification, it has multicopy nature and enables differentiation between species. The objective of this study was to show that the use of molecular biology tools associated with morphotyping to characterize species of ectomycorrhizae is more effective than when they are used on their own.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000300246&lng=en&tlng=enPCRectomycorrhizal fungimolecular identificationmorphotype |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bárbara Estevão Clasen Andressa de Oliveira Silveira Daiana Bortoluzzi Baldoni Daiane Fiuza Montagner Rodrigo Josemar Seminoti Jacques Zaida Inês Antoniolli |
spellingShingle |
Bárbara Estevão Clasen Andressa de Oliveira Silveira Daiana Bortoluzzi Baldoni Daiane Fiuza Montagner Rodrigo Josemar Seminoti Jacques Zaida Inês Antoniolli Characterization of Ectomycorrhizal species through molecular biology tools and morphotyping Scientia Agricola PCR ectomycorrhizal fungi molecular identification morphotype |
author_facet |
Bárbara Estevão Clasen Andressa de Oliveira Silveira Daiana Bortoluzzi Baldoni Daiane Fiuza Montagner Rodrigo Josemar Seminoti Jacques Zaida Inês Antoniolli |
author_sort |
Bárbara Estevão Clasen |
title |
Characterization of Ectomycorrhizal species through molecular biology tools and morphotyping |
title_short |
Characterization of Ectomycorrhizal species through molecular biology tools and morphotyping |
title_full |
Characterization of Ectomycorrhizal species through molecular biology tools and morphotyping |
title_fullStr |
Characterization of Ectomycorrhizal species through molecular biology tools and morphotyping |
title_full_unstemmed |
Characterization of Ectomycorrhizal species through molecular biology tools and morphotyping |
title_sort |
characterization of ectomycorrhizal species through molecular biology tools and morphotyping |
publisher |
Universidade de São Paulo |
series |
Scientia Agricola |
issn |
1678-992X |
description |
ABSTRACT: Mycorrhizae are mutualistic associations between fungi and plant roots. These symbiotic associations are abundant and occur in 75 to 80 % of plants. Ectomycorrhizal fungi are very important in ecosystems, because their mutualistic association with plants of different species helps nutrients and water absorption, as well as protection of the host plant against pathogens and abiotic stresses. Most ectomycorrhizal fungi belong to the Basidiomycota class, such as the following genera: Amanita, Hebeloma, Hysterangium, Laccaria, Lactarius, Rhizopogon, Russula, Scleroderma, Suillus, Tricholoma, among others. Morphological studies on ectomycorrhizae report important results in understanding the species biodiversity. However, the use of molecular biology nowadays is indispensable. Among the various molecular tools available, there is consensus about the use of tools based on sequencing of the Internal Transcribed Spacer (ITS) of fungi rDNA, aiding in species characterization and construction of phylogenetic studies. The ITS region is of easy amplification, it has multicopy nature and enables differentiation between species. The objective of this study was to show that the use of molecular biology tools associated with morphotyping to characterize species of ectomycorrhizae is more effective than when they are used on their own. |
topic |
PCR ectomycorrhizal fungi molecular identification morphotype |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000300246&lng=en&tlng=en |
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