Non-Enzymatic Glucose Biosensor Based on Highly Pure TiO₂ Nanoparticles

• Main Authors: Hongin Jeong, Jhongryul Yoo, Seokyung Park, Jiling Lu, Sungho Park, Jeseung Lee
• Format: Article
• Language: English
• Published: MDPI AG 2021-05-01
• Series: Biosensors
• Subjects: titanium dioxide; nanoparticle; deposition; glucose; biosensor
• Online Access: https://www.mdpi.com/2079-6374/11/5/149
• ISSN: 2079-6374

This study proposes a non-enzymatic glucose sensor fabricated by synthesizing high-purity TiO₂ nanoparticles in thermal plasma and depositing it directly on a substrate and then depositing chitosan–polypyrrole (CS-PPy) conductive polymer films by electrochemical method. The structural properties of the deposited TiO₂ nanoparticles were analyzed by X-ray diffraction (XRD) and dynamic light scattering (DLS) system. The chemical composition and structural properties of the TiO₂ nanoparticle layer and the conductive polymer films were confirmed by X-ray photoelectron spectroscopy (XPS) spectra and scanning electron microscope (SEM). The glucose detection characteristics of the fabricated biosensor were determined by cyclic voltammetry (CV). CS-PPy/TiO₂ biosensor showed high sensitivity of 302.0 µA mM⁻¹ cm⁻² (<i>R</i>² = 0.9957) and low detection limit of 6.7 μM. The easily manufactured CS-PPy/TiO₂ biosensor showed excellent selectivity and reactivity.