Extracts of <i>Phyllostachys pubescens</i> Leaves Represses Human Steroid 5-Alpha Reductase Type 2 Promoter Activity in BHP-1 Cells and Ameliorates Testosterone-Induced Benign Prostatic Hyperplasia in Rat Model
Benign prostatic hyperplasia (BPH) is the most common symptomatic abnormality of the human prostate characterized by uncontrolled proliferation of the prostate gland. In this study, we investigated the effect of bamboo, <i>Phyllostachys pubescens,</i> leaves extract (PPE) on human 5α-red...
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doaj-7db44b95c97c4f4892338a8164cc9d482021-03-10T00:04:51ZengMDPI AGNutrients2072-66432021-03-011388488410.3390/nu13030884Extracts of <i>Phyllostachys pubescens</i> Leaves Represses Human Steroid 5-Alpha Reductase Type 2 Promoter Activity in BHP-1 Cells and Ameliorates Testosterone-Induced Benign Prostatic Hyperplasia in Rat ModelKwang Hoon Song0Chang-Seob Seo1Won-Kyung Yang2Hyun-O Gu3Ki-Joong Kim4Seung-Hyung Kim5Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Yuseong-daero 1672, Yuseong-gu, Daejeon 34054, KoreaHerbal Medicine Research Division, Korea Institute of Oriental Medicine, Yuseong-daero 1672, Yuseong-gu, Daejeon 34054, KoreaInstitute of Traditional Medicine and Bioscience, Daejeon University, Daejeon 34520, KoreaInstitute of Traditional Medicine and Bioscience, Daejeon University, Daejeon 34520, KoreaInstitute of Traditional Medicine and Bioscience, Daejeon University, Daejeon 34520, KoreaInstitute of Traditional Medicine and Bioscience, Daejeon University, Daejeon 34520, KoreaBenign prostatic hyperplasia (BPH) is the most common symptomatic abnormality of the human prostate characterized by uncontrolled proliferation of the prostate gland. In this study, we investigated the effect of bamboo, <i>Phyllostachys pubescens,</i> leaves extract (PPE) on human 5α-reductase type 2 (SRD5A2) gene promoter activity in human prostate cell lines and the protective effect of PPE on a testosterone-induced BPH rat model. PPE repressed human SRD5A2 promoter activity and its mRNA expression. The rats treated with PPE for 4 weeks showed a significantly attenuated prostate weight compared to vehicle control. PPE-treated rats also showed reduced serum dihydrotestosterone, testosterone, prostate-specific antigen, and SRD5A2 levels by testosterone injection. Quantitative real-time polymerase chain reaction showed that PPE treatment significantly decreased mRNA expression of SRD5A2, androgen receptor (AR), proliferating cell nuclear antigen (PCNA), and fibroblast growth factor 2 compared with the vehicle-treated, testosterone-injected rats in the prostate. Furthermore, PPE treatment showed reduced AR, PCNA, and tumor necrosis factor alpha expression in the prostate via immunohistofluorescence staining. In conclusion, oral administration of PPE prevented and inhibited the development and progression of enlarged prostate lesions in testosterone-induced animal models through various anti-proliferative and anti-inflammatory pharmacological effects and induced suppression of SRD5A2 gene expression.https://www.mdpi.com/2072-6643/13/3/884<i>Phyllostachys pubescens</i>benign prostatic hyperplasia5α-reductase type 2dihydrotestosterone |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kwang Hoon Song Chang-Seob Seo Won-Kyung Yang Hyun-O Gu Ki-Joong Kim Seung-Hyung Kim |
spellingShingle |
Kwang Hoon Song Chang-Seob Seo Won-Kyung Yang Hyun-O Gu Ki-Joong Kim Seung-Hyung Kim Extracts of <i>Phyllostachys pubescens</i> Leaves Represses Human Steroid 5-Alpha Reductase Type 2 Promoter Activity in BHP-1 Cells and Ameliorates Testosterone-Induced Benign Prostatic Hyperplasia in Rat Model Nutrients <i>Phyllostachys pubescens</i> benign prostatic hyperplasia 5α-reductase type 2 dihydrotestosterone |
author_facet |
Kwang Hoon Song Chang-Seob Seo Won-Kyung Yang Hyun-O Gu Ki-Joong Kim Seung-Hyung Kim |
author_sort |
Kwang Hoon Song |
title |
Extracts of <i>Phyllostachys pubescens</i> Leaves Represses Human Steroid 5-Alpha Reductase Type 2 Promoter Activity in BHP-1 Cells and Ameliorates Testosterone-Induced Benign Prostatic Hyperplasia in Rat Model |
title_short |
Extracts of <i>Phyllostachys pubescens</i> Leaves Represses Human Steroid 5-Alpha Reductase Type 2 Promoter Activity in BHP-1 Cells and Ameliorates Testosterone-Induced Benign Prostatic Hyperplasia in Rat Model |
title_full |
Extracts of <i>Phyllostachys pubescens</i> Leaves Represses Human Steroid 5-Alpha Reductase Type 2 Promoter Activity in BHP-1 Cells and Ameliorates Testosterone-Induced Benign Prostatic Hyperplasia in Rat Model |
title_fullStr |
Extracts of <i>Phyllostachys pubescens</i> Leaves Represses Human Steroid 5-Alpha Reductase Type 2 Promoter Activity in BHP-1 Cells and Ameliorates Testosterone-Induced Benign Prostatic Hyperplasia in Rat Model |
title_full_unstemmed |
Extracts of <i>Phyllostachys pubescens</i> Leaves Represses Human Steroid 5-Alpha Reductase Type 2 Promoter Activity in BHP-1 Cells and Ameliorates Testosterone-Induced Benign Prostatic Hyperplasia in Rat Model |
title_sort |
extracts of <i>phyllostachys pubescens</i> leaves represses human steroid 5-alpha reductase type 2 promoter activity in bhp-1 cells and ameliorates testosterone-induced benign prostatic hyperplasia in rat model |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2021-03-01 |
description |
Benign prostatic hyperplasia (BPH) is the most common symptomatic abnormality of the human prostate characterized by uncontrolled proliferation of the prostate gland. In this study, we investigated the effect of bamboo, <i>Phyllostachys pubescens,</i> leaves extract (PPE) on human 5α-reductase type 2 (SRD5A2) gene promoter activity in human prostate cell lines and the protective effect of PPE on a testosterone-induced BPH rat model. PPE repressed human SRD5A2 promoter activity and its mRNA expression. The rats treated with PPE for 4 weeks showed a significantly attenuated prostate weight compared to vehicle control. PPE-treated rats also showed reduced serum dihydrotestosterone, testosterone, prostate-specific antigen, and SRD5A2 levels by testosterone injection. Quantitative real-time polymerase chain reaction showed that PPE treatment significantly decreased mRNA expression of SRD5A2, androgen receptor (AR), proliferating cell nuclear antigen (PCNA), and fibroblast growth factor 2 compared with the vehicle-treated, testosterone-injected rats in the prostate. Furthermore, PPE treatment showed reduced AR, PCNA, and tumor necrosis factor alpha expression in the prostate via immunohistofluorescence staining. In conclusion, oral administration of PPE prevented and inhibited the development and progression of enlarged prostate lesions in testosterone-induced animal models through various anti-proliferative and anti-inflammatory pharmacological effects and induced suppression of SRD5A2 gene expression. |
topic |
<i>Phyllostachys pubescens</i> benign prostatic hyperplasia 5α-reductase type 2 dihydrotestosterone |
url |
https://www.mdpi.com/2072-6643/13/3/884 |
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