Application of a double-enrichment procedure for microsatellite isolation and the use of tailed primers for high throughput genotyping

Application of a double-enrichment procedure for microsatellite isolation and the use of tailed primers for high throughput genotyping

The number of microsatellite loci and their allelic diversity contribute to increase accuracy and informativity of genetic estimates, however, the isolation of microsatellite loci is not only laborious but also quite expensive. We used (GATA)n and (GACA)n tetranucleotide probes and single- and doubl...

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Bibliographic Details
Main Authors: Fábio Mendonça Diniz, Arati Iyengar, Paulo Sarmanho da Costa Lima, Norman Maclean, Paul Bentzen
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2007-03-01
Series:Genetics and Molecular Biology
Subjects:
double-enrichment
double-hybridization
stutter bands
tailed microsatellite primers
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300014

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