Placenta-specific novel splice variants of Rho GDP dissociation inhibitor β are highly expressed in cancerous cells
<p>Abstract</p> <p>Background</p> <p>Alternative splicing of pre-mRNA transcripts not only plays a role in normal molecular processes but is also associated with cancer development. While normal transcripts are ubiquitously expressed in normal tissues, splice variants c...
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doaj-7dc8437bea534c21858ee62e7fa274e02020-11-25T03:28:29ZengBMCBMC Research Notes1756-05002012-12-015166610.1186/1756-0500-5-666Placenta-specific novel splice variants of Rho GDP dissociation inhibitor β are highly expressed in cancerous cellsHatakeyama KeiichiFukuda YorikaneOhshima KeiichiTerashima MasanoriYamaguchi KenMochizuki Tohru<p>Abstract</p> <p>Background</p> <p>Alternative splicing of pre-mRNA transcripts not only plays a role in normal molecular processes but is also associated with cancer development. While normal transcripts are ubiquitously expressed in normal tissues, splice variants created through abnormal alternative splicing events are often expressed in cancer cells. Although the Rho GDP dissociation inhibitor β (<it>ARHGDIB</it>) gene has been found to be ubiquitously expressed in normal tissues and involved in cancer development, the presence of splice variants of <it>ARHGDIB</it> has not yet been investigated.</p> <p>Results</p> <p>Validation analysis for the presence of and exon structures of splice variants of <it>ARHGDIB</it>, performed using reverse-transcriptase polymerase chain reaction and DNA sequencing, successfully identified novel splice variants of <it>ARHGDIB</it>, that is, 6a, 6b, and 6c, in colon, pancreas, stomach, and breast cancer cell lines. Quantitative real-time polymerase chain reaction analysis showed that these variants were also highly expressed in normal placental tissue but not in other types of normal tissue.</p> <p>Conclusions</p> <p>Expression of ARHGDIB variants 6a, 6b, and 6c appears to be restricted to cancer cells and normal placental tissue, suggesting that these variants possess cancer-specific functions and, as such, are potential cancer-related biomarkers.</p> http://www.biomedcentral.com/1756-0500/5/666Alternative splicingARHGDIBBiomarkerMetastasisSplice variant |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hatakeyama Keiichi Fukuda Yorikane Ohshima Keiichi Terashima Masanori Yamaguchi Ken Mochizuki Tohru |
spellingShingle |
Hatakeyama Keiichi Fukuda Yorikane Ohshima Keiichi Terashima Masanori Yamaguchi Ken Mochizuki Tohru Placenta-specific novel splice variants of Rho GDP dissociation inhibitor β are highly expressed in cancerous cells BMC Research Notes Alternative splicing ARHGDIB Biomarker Metastasis Splice variant |
author_facet |
Hatakeyama Keiichi Fukuda Yorikane Ohshima Keiichi Terashima Masanori Yamaguchi Ken Mochizuki Tohru |
author_sort |
Hatakeyama Keiichi |
title |
Placenta-specific novel splice variants of Rho GDP dissociation inhibitor β are highly expressed in cancerous cells |
title_short |
Placenta-specific novel splice variants of Rho GDP dissociation inhibitor β are highly expressed in cancerous cells |
title_full |
Placenta-specific novel splice variants of Rho GDP dissociation inhibitor β are highly expressed in cancerous cells |
title_fullStr |
Placenta-specific novel splice variants of Rho GDP dissociation inhibitor β are highly expressed in cancerous cells |
title_full_unstemmed |
Placenta-specific novel splice variants of Rho GDP dissociation inhibitor β are highly expressed in cancerous cells |
title_sort |
placenta-specific novel splice variants of rho gdp dissociation inhibitor β are highly expressed in cancerous cells |
publisher |
BMC |
series |
BMC Research Notes |
issn |
1756-0500 |
publishDate |
2012-12-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Alternative splicing of pre-mRNA transcripts not only plays a role in normal molecular processes but is also associated with cancer development. While normal transcripts are ubiquitously expressed in normal tissues, splice variants created through abnormal alternative splicing events are often expressed in cancer cells. Although the Rho GDP dissociation inhibitor β (<it>ARHGDIB</it>) gene has been found to be ubiquitously expressed in normal tissues and involved in cancer development, the presence of splice variants of <it>ARHGDIB</it> has not yet been investigated.</p> <p>Results</p> <p>Validation analysis for the presence of and exon structures of splice variants of <it>ARHGDIB</it>, performed using reverse-transcriptase polymerase chain reaction and DNA sequencing, successfully identified novel splice variants of <it>ARHGDIB</it>, that is, 6a, 6b, and 6c, in colon, pancreas, stomach, and breast cancer cell lines. Quantitative real-time polymerase chain reaction analysis showed that these variants were also highly expressed in normal placental tissue but not in other types of normal tissue.</p> <p>Conclusions</p> <p>Expression of ARHGDIB variants 6a, 6b, and 6c appears to be restricted to cancer cells and normal placental tissue, suggesting that these variants possess cancer-specific functions and, as such, are potential cancer-related biomarkers.</p> |
topic |
Alternative splicing ARHGDIB Biomarker Metastasis Splice variant |
url |
http://www.biomedcentral.com/1756-0500/5/666 |
work_keys_str_mv |
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