Effects of clenbuterol administration on mitochondrial morphology and its regulatory proteins in rat skeletal muscle
Abstract Clenbuterol induces a slow‐to‐fast fiber type transition in skeletal muscle. This muscle fiber transition decreased mitochondrial oxidative capacity and respiratory function. We hypothesized that the clenbuterol‐mediated reduction in oxidative capacity is associated with the alteration in m...
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doaj-7dcd861e8bbd4a4c8c0f29d879614d3d2020-11-25T01:29:41ZengWileyPhysiological Reports2051-817X2019-10-01719n/an/a10.14814/phy2.14266Effects of clenbuterol administration on mitochondrial morphology and its regulatory proteins in rat skeletal muscleYu Kitaoka0Daiki Watanabe1Yudai Nonaka2Kazuyoshi Yagishita3Yutaka Kano4Daisuke Hoshino5Department of Human Sciences Kanagawa University Yokohama JapanGraduate School of Integrated Arts and Sciences Hiroshima University Hiroshima JapanDepartment of Engineering Science The University of Electro‐communications Chofu Tokyo JapanClinical Center for Sports Medicine and Sports Dentistry Hyperbaric Medical Center/Sports Medicine Clinical Center Medical Hospital of Tokyo Medical and Dental University Bunkyo‐ku Tokyo JapanDepartment of Engineering Science The University of Electro‐communications Chofu Tokyo JapanDepartment of Engineering Science The University of Electro‐communications Chofu Tokyo JapanAbstract Clenbuterol induces a slow‐to‐fast fiber type transition in skeletal muscle. This muscle fiber transition decreased mitochondrial oxidative capacity and respiratory function. We hypothesized that the clenbuterol‐mediated reduction in oxidative capacity is associated with the alteration in mitochondrial morphology. To verify this hypothesis, we examined whether clenbuterol alters mitochondrial morphology and mitochondrial regulatory proteins in rat skeletal muscle. Clenbuterol was administered to rats via drinking water (30 mg/L) for 3 weeks. Myosin heavy chain (MHC) isoform composition, mitochondrial morphology, and fusion and fission regulatory protein levels in deep region and superficial region in tibialis anterior (TA) muscles were assessed. Clenbuterol induced the fiber type transition from slow to fast in both the regions of TA. The levels of optic atrophy protein 1, mitofusin 2, and mitochondrial fission 1, but not of dynamin‐related protein 1, significantly decreased in deep and superficial muscles after clenbuterol administration (P < 0.01). Also, observation using the transmission electron microscopy showed a decrease in mitochondrial volume (P < 0.05) and an increase in proportion of continuous or interacting mitochondria across Z‐lines (P < 0.05). We showed that clenbuterol administration induces a transition in the muscle fiber type composition toward fast phenotype and causes alterations in mitochondrial morphology with a concomitant decrease in mitochondrial fusion and fission regulatory protein levels. These mitochondrial morphological alterations may influence deleterious effects on skeletal muscle metabolism.https://doi.org/10.14814/phy2.14266β2 adrenergic agonistmuscle fiber typemitochondrial dynamics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yu Kitaoka Daiki Watanabe Yudai Nonaka Kazuyoshi Yagishita Yutaka Kano Daisuke Hoshino |
spellingShingle |
Yu Kitaoka Daiki Watanabe Yudai Nonaka Kazuyoshi Yagishita Yutaka Kano Daisuke Hoshino Effects of clenbuterol administration on mitochondrial morphology and its regulatory proteins in rat skeletal muscle Physiological Reports β2 adrenergic agonist muscle fiber type mitochondrial dynamics |
author_facet |
Yu Kitaoka Daiki Watanabe Yudai Nonaka Kazuyoshi Yagishita Yutaka Kano Daisuke Hoshino |
author_sort |
Yu Kitaoka |
title |
Effects of clenbuterol administration on mitochondrial morphology and its regulatory proteins in rat skeletal muscle |
title_short |
Effects of clenbuterol administration on mitochondrial morphology and its regulatory proteins in rat skeletal muscle |
title_full |
Effects of clenbuterol administration on mitochondrial morphology and its regulatory proteins in rat skeletal muscle |
title_fullStr |
Effects of clenbuterol administration on mitochondrial morphology and its regulatory proteins in rat skeletal muscle |
title_full_unstemmed |
Effects of clenbuterol administration on mitochondrial morphology and its regulatory proteins in rat skeletal muscle |
title_sort |
effects of clenbuterol administration on mitochondrial morphology and its regulatory proteins in rat skeletal muscle |
publisher |
Wiley |
series |
Physiological Reports |
issn |
2051-817X |
publishDate |
2019-10-01 |
description |
Abstract Clenbuterol induces a slow‐to‐fast fiber type transition in skeletal muscle. This muscle fiber transition decreased mitochondrial oxidative capacity and respiratory function. We hypothesized that the clenbuterol‐mediated reduction in oxidative capacity is associated with the alteration in mitochondrial morphology. To verify this hypothesis, we examined whether clenbuterol alters mitochondrial morphology and mitochondrial regulatory proteins in rat skeletal muscle. Clenbuterol was administered to rats via drinking water (30 mg/L) for 3 weeks. Myosin heavy chain (MHC) isoform composition, mitochondrial morphology, and fusion and fission regulatory protein levels in deep region and superficial region in tibialis anterior (TA) muscles were assessed. Clenbuterol induced the fiber type transition from slow to fast in both the regions of TA. The levels of optic atrophy protein 1, mitofusin 2, and mitochondrial fission 1, but not of dynamin‐related protein 1, significantly decreased in deep and superficial muscles after clenbuterol administration (P < 0.01). Also, observation using the transmission electron microscopy showed a decrease in mitochondrial volume (P < 0.05) and an increase in proportion of continuous or interacting mitochondria across Z‐lines (P < 0.05). We showed that clenbuterol administration induces a transition in the muscle fiber type composition toward fast phenotype and causes alterations in mitochondrial morphology with a concomitant decrease in mitochondrial fusion and fission regulatory protein levels. These mitochondrial morphological alterations may influence deleterious effects on skeletal muscle metabolism. |
topic |
β2 adrenergic agonist muscle fiber type mitochondrial dynamics |
url |
https://doi.org/10.14814/phy2.14266 |
work_keys_str_mv |
AT yukitaoka effectsofclenbuteroladministrationonmitochondrialmorphologyanditsregulatoryproteinsinratskeletalmuscle AT daikiwatanabe effectsofclenbuteroladministrationonmitochondrialmorphologyanditsregulatoryproteinsinratskeletalmuscle AT yudainonaka effectsofclenbuteroladministrationonmitochondrialmorphologyanditsregulatoryproteinsinratskeletalmuscle AT kazuyoshiyagishita effectsofclenbuteroladministrationonmitochondrialmorphologyanditsregulatoryproteinsinratskeletalmuscle AT yutakakano effectsofclenbuteroladministrationonmitochondrialmorphologyanditsregulatoryproteinsinratskeletalmuscle AT daisukehoshino effectsofclenbuteroladministrationonmitochondrialmorphologyanditsregulatoryproteinsinratskeletalmuscle |
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1725095552594477056 |