Influence of miRNA-30a-5p on Pulmonary Fibrosis in Mice with Streptococcus pneumoniae Infection through Regulation of Autophagy by Beclin-1
The study is aimed at observing the influence of microribonucleic acid- (miRNA-) 30a-50p on the pulmonary fibrosis in mice with Streptococcus pneumoniae infection through the regulation of autophagy by Beclin-1. Specific pathogen-free mice were instilled with Streptococcus pneumoniae through the tra...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2021-01-01
|
Series: | BioMed Research International |
Online Access: | http://dx.doi.org/10.1155/2021/9963700 |
id |
doaj-7deb1f31017346db804fb81a381d9eb9 |
---|---|
record_format |
Article |
spelling |
doaj-7deb1f31017346db804fb81a381d9eb92021-10-04T01:58:45ZengHindawi LimitedBioMed Research International2314-61412021-01-01202110.1155/2021/9963700Influence of miRNA-30a-5p on Pulmonary Fibrosis in Mice with Streptococcus pneumoniae Infection through Regulation of Autophagy by Beclin-1Hanyu Liu0Yabo Li1Yingdong Zou2Xingzong Zhang3Xiongfei Shi4Zhiping Yin5Yun Lin6Department of LaboratoryDepartment of LaboratoryDepartment of LaboratoryDepartment of LaboratoryDepartment of LaboratoryDepartment of LaboratoryDepartment of LaboratoryThe study is aimed at observing the influence of microribonucleic acid- (miRNA-) 30a-50p on the pulmonary fibrosis in mice with Streptococcus pneumoniae infection through the regulation of autophagy by Beclin-1. Specific pathogen-free mice were instilled with Streptococcus pneumoniae through the trachea to establish the pulmonary fibrosis model. Then, they were divided into the miRNA-30a-50p mimics group (mimics group, n=10) and miRNA-30a-5p inhibitors group (inhibitors group, n=10), with the control group (n=10) also set. Pulmonary tissue wet weight/dry weight (W/D) was detected. The content of tumor necrosis factor-α (TNF-α), interleukin- (IL-) 6, and myeloperoxidase (MPO) was determined using enzyme-linked immunosorbent assay (ELISA). Besides, the changes in the pulmonary function index dynamic lung compliance (Cdyn), plateau pressure (Pplat), and peak airway pressure (Ppeak) were monitored, and the gene and protein expression levels were measured via quantitative PCR (qPCR) and Western blotting. The expression level of miRNA-30a-5p was substantially raised in the mimics group (p<0.05), but extremely low in the inhibitors group (p<0.05). The mimics group had obviously raised levels of serum aminotransferase (AST), glutamic-pyruvic transaminase (GPT), alkaline phosphatase (ALP), and pulmonary tissue W/D (p<0.05). Additionally, the expression levels of TNF-α, IL-6, and MPO were notably elevated in the mimics group, while their expression levels showed the opposite conditions in the inhibitors group (p<0.05). According to the HE staining results, the inhibitors group had arranged orderly cells, while the mimics group exhibited lung injury, pulmonary edema, severe inflammatory response, and alveolar congestion. In the inhibitors group, Cdyn was remarkably elevated, but Pplat and Ppeak declined considerably (p<0.05). Besides, the inhibitors group exhibited elevated messenger RNA (mRNA) levels of Beclin-1 and LC3, lowered mRNA levels of α-SMA and p62, a raised protein level of Beclin-1, and a markedly decreased protein level of p62 (p<0.05). Silencing miRNA-30a-5p expression can promote the expression of Beclin-1 to accelerate the occurrence of autophagy, thereby treating pulmonary fibrosis in mice with Streptococcus pneumoniae infection.http://dx.doi.org/10.1155/2021/9963700 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hanyu Liu Yabo Li Yingdong Zou Xingzong Zhang Xiongfei Shi Zhiping Yin Yun Lin |
spellingShingle |
Hanyu Liu Yabo Li Yingdong Zou Xingzong Zhang Xiongfei Shi Zhiping Yin Yun Lin Influence of miRNA-30a-5p on Pulmonary Fibrosis in Mice with Streptococcus pneumoniae Infection through Regulation of Autophagy by Beclin-1 BioMed Research International |
author_facet |
Hanyu Liu Yabo Li Yingdong Zou Xingzong Zhang Xiongfei Shi Zhiping Yin Yun Lin |
author_sort |
Hanyu Liu |
title |
Influence of miRNA-30a-5p on Pulmonary Fibrosis in Mice with Streptococcus pneumoniae Infection through Regulation of Autophagy by Beclin-1 |
title_short |
Influence of miRNA-30a-5p on Pulmonary Fibrosis in Mice with Streptococcus pneumoniae Infection through Regulation of Autophagy by Beclin-1 |
title_full |
Influence of miRNA-30a-5p on Pulmonary Fibrosis in Mice with Streptococcus pneumoniae Infection through Regulation of Autophagy by Beclin-1 |
title_fullStr |
Influence of miRNA-30a-5p on Pulmonary Fibrosis in Mice with Streptococcus pneumoniae Infection through Regulation of Autophagy by Beclin-1 |
title_full_unstemmed |
Influence of miRNA-30a-5p on Pulmonary Fibrosis in Mice with Streptococcus pneumoniae Infection through Regulation of Autophagy by Beclin-1 |
title_sort |
influence of mirna-30a-5p on pulmonary fibrosis in mice with streptococcus pneumoniae infection through regulation of autophagy by beclin-1 |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6141 |
publishDate |
2021-01-01 |
description |
The study is aimed at observing the influence of microribonucleic acid- (miRNA-) 30a-50p on the pulmonary fibrosis in mice with Streptococcus pneumoniae infection through the regulation of autophagy by Beclin-1. Specific pathogen-free mice were instilled with Streptococcus pneumoniae through the trachea to establish the pulmonary fibrosis model. Then, they were divided into the miRNA-30a-50p mimics group (mimics group, n=10) and miRNA-30a-5p inhibitors group (inhibitors group, n=10), with the control group (n=10) also set. Pulmonary tissue wet weight/dry weight (W/D) was detected. The content of tumor necrosis factor-α (TNF-α), interleukin- (IL-) 6, and myeloperoxidase (MPO) was determined using enzyme-linked immunosorbent assay (ELISA). Besides, the changes in the pulmonary function index dynamic lung compliance (Cdyn), plateau pressure (Pplat), and peak airway pressure (Ppeak) were monitored, and the gene and protein expression levels were measured via quantitative PCR (qPCR) and Western blotting. The expression level of miRNA-30a-5p was substantially raised in the mimics group (p<0.05), but extremely low in the inhibitors group (p<0.05). The mimics group had obviously raised levels of serum aminotransferase (AST), glutamic-pyruvic transaminase (GPT), alkaline phosphatase (ALP), and pulmonary tissue W/D (p<0.05). Additionally, the expression levels of TNF-α, IL-6, and MPO were notably elevated in the mimics group, while their expression levels showed the opposite conditions in the inhibitors group (p<0.05). According to the HE staining results, the inhibitors group had arranged orderly cells, while the mimics group exhibited lung injury, pulmonary edema, severe inflammatory response, and alveolar congestion. In the inhibitors group, Cdyn was remarkably elevated, but Pplat and Ppeak declined considerably (p<0.05). Besides, the inhibitors group exhibited elevated messenger RNA (mRNA) levels of Beclin-1 and LC3, lowered mRNA levels of α-SMA and p62, a raised protein level of Beclin-1, and a markedly decreased protein level of p62 (p<0.05). Silencing miRNA-30a-5p expression can promote the expression of Beclin-1 to accelerate the occurrence of autophagy, thereby treating pulmonary fibrosis in mice with Streptococcus pneumoniae infection. |
url |
http://dx.doi.org/10.1155/2021/9963700 |
work_keys_str_mv |
AT hanyuliu influenceofmirna30a5ponpulmonaryfibrosisinmicewithstreptococcuspneumoniaeinfectionthroughregulationofautophagybybeclin1 AT yaboli influenceofmirna30a5ponpulmonaryfibrosisinmicewithstreptococcuspneumoniaeinfectionthroughregulationofautophagybybeclin1 AT yingdongzou influenceofmirna30a5ponpulmonaryfibrosisinmicewithstreptococcuspneumoniaeinfectionthroughregulationofautophagybybeclin1 AT xingzongzhang influenceofmirna30a5ponpulmonaryfibrosisinmicewithstreptococcuspneumoniaeinfectionthroughregulationofautophagybybeclin1 AT xiongfeishi influenceofmirna30a5ponpulmonaryfibrosisinmicewithstreptococcuspneumoniaeinfectionthroughregulationofautophagybybeclin1 AT zhipingyin influenceofmirna30a5ponpulmonaryfibrosisinmicewithstreptococcuspneumoniaeinfectionthroughregulationofautophagybybeclin1 AT yunlin influenceofmirna30a5ponpulmonaryfibrosisinmicewithstreptococcuspneumoniaeinfectionthroughregulationofautophagybybeclin1 |
_version_ |
1716844665347506176 |