Evaluation of different carbon sources for high frequency callus culture with reduced phenolic secretion in cotton (Gossypium hirsutum L.) cv. SVPR-2
An efficient protocol was developed to control excessive phenolic compound secretion during callus culture of cotton. As cotton is naturally rich in phenolic compounds factors influencing the phenolic compound secretion, callus induction and proliferation were optimized for getting high frequency ca...
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doaj-7df3f7d30ca8490c90c13511b4fdde5c2020-11-24T20:57:45ZengElsevierBiotechnology Reports2215-017X2015-09-017C728010.1016/j.btre.2015.05.005Evaluation of different carbon sources for high frequency callus culture with reduced phenolic secretion in cotton (Gossypium hirsutum L.) cv. SVPR-2G. Prem Kumar0Sivakumar Subiramani1Siva Govindarajan2Vinoth Sadasivam3Vigneswaran Manickam4Kanakachari Mogilicherla5Senthil Kumar Thiruppathi6Jayabalan Narayanasamy7Department of Plant Science, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, IndiaDepartment of Plant Science, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, IndiaDepartment of Plant Science, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, IndiaDepartment of Plant Science, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, IndiaDepartment of Plant Science, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, IndiaDepartment of Plant Science, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, IndiaDepartment of Industry-University Collaboration, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, IndiaDepartment of Plant Science, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, IndiaAn efficient protocol was developed to control excessive phenolic compound secretion during callus culture of cotton. As cotton is naturally rich in phenolic compounds factors influencing the phenolic compound secretion, callus induction and proliferation were optimized for getting high frequency callus culture. Different carbon sources such as fructose, glucose, sucrose and maltose were tested at various concentrations to control phenolic secretion in callus culture. Among them, 3% maltose was found to be the best carbon source for effectively controlling phenolic secretion in callus induction medium. High frequency of callus induction was obtained on MSB5 medium supplemented with 3% Maltose, 2,4-D (0.90 μM) and Kinetin (4.60 μM) from both cotyledon and hypocotyl explants. The best result of callus induction was obtained with hypocotyl explant (94.90%) followed by cotyledon explant (85.20%). MSB5 medium supplemented with 2,4-D (0.45 μM) along with 2iP (2.95 μM) gave tremendous proliferation of callus with high percentage of response. Varying degrees of colors and textures of callus were observed under different hormone treatments. The present study offers a solution for controlling phenolic secretion in cotton callus culture by adjusting carbon sources without adding any additives and evaluates the manipulation of plant growth regulators for efficient callus culture of SVPR-2 cotton cultivar.http://www.sciencedirect.com/science/article/pii/S2215017X15000326CottonSVPR-2Phenolic secretionCallus cultureCarbon sourcesMSB5 medium |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
G. Prem Kumar Sivakumar Subiramani Siva Govindarajan Vinoth Sadasivam Vigneswaran Manickam Kanakachari Mogilicherla Senthil Kumar Thiruppathi Jayabalan Narayanasamy |
spellingShingle |
G. Prem Kumar Sivakumar Subiramani Siva Govindarajan Vinoth Sadasivam Vigneswaran Manickam Kanakachari Mogilicherla Senthil Kumar Thiruppathi Jayabalan Narayanasamy Evaluation of different carbon sources for high frequency callus culture with reduced phenolic secretion in cotton (Gossypium hirsutum L.) cv. SVPR-2 Biotechnology Reports Cotton SVPR-2 Phenolic secretion Callus culture Carbon sources MSB5 medium |
author_facet |
G. Prem Kumar Sivakumar Subiramani Siva Govindarajan Vinoth Sadasivam Vigneswaran Manickam Kanakachari Mogilicherla Senthil Kumar Thiruppathi Jayabalan Narayanasamy |
author_sort |
G. Prem Kumar |
title |
Evaluation of different carbon sources for high frequency callus culture with reduced phenolic secretion in cotton (Gossypium hirsutum L.) cv. SVPR-2 |
title_short |
Evaluation of different carbon sources for high frequency callus culture with reduced phenolic secretion in cotton (Gossypium hirsutum L.) cv. SVPR-2 |
title_full |
Evaluation of different carbon sources for high frequency callus culture with reduced phenolic secretion in cotton (Gossypium hirsutum L.) cv. SVPR-2 |
title_fullStr |
Evaluation of different carbon sources for high frequency callus culture with reduced phenolic secretion in cotton (Gossypium hirsutum L.) cv. SVPR-2 |
title_full_unstemmed |
Evaluation of different carbon sources for high frequency callus culture with reduced phenolic secretion in cotton (Gossypium hirsutum L.) cv. SVPR-2 |
title_sort |
evaluation of different carbon sources for high frequency callus culture with reduced phenolic secretion in cotton (gossypium hirsutum l.) cv. svpr-2 |
publisher |
Elsevier |
series |
Biotechnology Reports |
issn |
2215-017X |
publishDate |
2015-09-01 |
description |
An efficient protocol was developed to control excessive phenolic compound secretion during callus culture of cotton. As cotton is naturally rich in phenolic compounds factors influencing the phenolic compound secretion, callus induction and proliferation were optimized for getting high frequency callus culture. Different carbon sources such as fructose, glucose, sucrose and maltose were tested at various concentrations to control phenolic secretion in callus culture. Among them, 3% maltose was found to be the best carbon source for effectively controlling phenolic secretion in callus induction medium. High frequency of callus induction was obtained on MSB5 medium supplemented with 3% Maltose, 2,4-D (0.90 μM) and Kinetin (4.60 μM) from both cotyledon and hypocotyl explants. The best result of callus induction was obtained with hypocotyl explant (94.90%) followed by cotyledon explant (85.20%). MSB5 medium supplemented with 2,4-D (0.45 μM) along with 2iP (2.95 μM) gave tremendous proliferation of callus with high percentage of response. Varying degrees of colors and textures of callus were observed under different hormone treatments. The present study offers a solution for controlling phenolic secretion in cotton callus culture by adjusting carbon sources without adding any additives and evaluates the manipulation of plant growth regulators for efficient callus culture of SVPR-2 cotton cultivar. |
topic |
Cotton SVPR-2 Phenolic secretion Callus culture Carbon sources MSB5 medium |
url |
http://www.sciencedirect.com/science/article/pii/S2215017X15000326 |
work_keys_str_mv |
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