Prevalence of Toxoplasma gondii Infection among Healthy Blood Donors in Northeast of Iran
Background: This cross-sectional investigation aimed to evaluate the prevalence of IgM and IgG anti- Toxoplasma gondii antibodies and the associated risk factors among healthy blood donors in Khorasan Razavi Province, northeast of Iran from Nov 2014 to May 2015. Methods: Overall, 491 serum samples...
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doaj-7df7fa581d9c49629b71c398fb5c2fc62021-04-02T12:16:27ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2017-12-01124705Prevalence of Toxoplasma gondii Infection among Healthy Blood Donors in Northeast of IranSaeed SADOOGHIAN0Hossein MAHMOUDVAND1Mohammad Ali MOHAMMADI2Naser NAZARI SARCHESHMEH3Amir TAVAKOLI KARESHK4Hossein KAMIABI5Naser ZIA-ALI6Dept. of Medical Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, IranDept. of Medical Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, IranBlood Transfusion Organization of Khorasan Razavi, Mashhad, IranBlood Transfusion Organization of Khorasan Razavi, Mashhad, IranDept. of Medical Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, IranDept. of Medical Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, IranDept. of Medical Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, Iran Background: This cross-sectional investigation aimed to evaluate the prevalence of IgM and IgG anti- Toxoplasma gondii antibodies and the associated risk factors among healthy blood donors in Khorasan Razavi Province, northeast of Iran from Nov 2014 to May 2015. Methods: Overall, 491 serum samples from apparently healthy blood donors referred the six biggest blood centers in Razavi Khorasan, Iran, were screened for IgG and IgM anti-T. gondii antibodies by enzyme-linked immunosorbent assay (ELISA). A structured questionnaire was used to obtain information on risk factors for T. gondii infection. Nested PCR was also used to detect DNA of T. gondii in the IgM-positive samples by using of B1 and RE (Repetitive Element) as marker for amplifying fragment size of 531 bp and 164 bp in PCR method. Results: Totally, 200 (40.7%) samples were seropositive for anti-T. gondii antibodies; 184 (37.5%) donors tested seropositive for only IgG antibody, 8 (1.6%) tested seropositive for both IgM and IgG and 8 (1.6%) were positive for IgM antibody alone. Several risk factors significantly related to T. gondii seropositivity in the univariate analysis at P<0.05 included age (P<0.001), and raw/half-cocked meat consumption (P=0.015). T. gondii DNA was found in all sixteen IgM-positive samples. Conclusion: T. gondii infection was present among healthy blood donors in northeast of Iran. Thus, it is suggested to design screening programs for preventing transfusion-transmitted toxoplasmosis. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/1929ToxoplasmosisBlood transfusionIgG antibodyIgM antibodyNested-PCRIran |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Saeed SADOOGHIAN Hossein MAHMOUDVAND Mohammad Ali MOHAMMADI Naser NAZARI SARCHESHMEH Amir TAVAKOLI KARESHK Hossein KAMIABI Naser ZIA-ALI |
spellingShingle |
Saeed SADOOGHIAN Hossein MAHMOUDVAND Mohammad Ali MOHAMMADI Naser NAZARI SARCHESHMEH Amir TAVAKOLI KARESHK Hossein KAMIABI Naser ZIA-ALI Prevalence of Toxoplasma gondii Infection among Healthy Blood Donors in Northeast of Iran Iranian Journal of Parasitology Toxoplasmosis Blood transfusion IgG antibody IgM antibody Nested-PCR Iran |
author_facet |
Saeed SADOOGHIAN Hossein MAHMOUDVAND Mohammad Ali MOHAMMADI Naser NAZARI SARCHESHMEH Amir TAVAKOLI KARESHK Hossein KAMIABI Naser ZIA-ALI |
author_sort |
Saeed SADOOGHIAN |
title |
Prevalence of Toxoplasma gondii Infection among Healthy Blood Donors in Northeast of Iran |
title_short |
Prevalence of Toxoplasma gondii Infection among Healthy Blood Donors in Northeast of Iran |
title_full |
Prevalence of Toxoplasma gondii Infection among Healthy Blood Donors in Northeast of Iran |
title_fullStr |
Prevalence of Toxoplasma gondii Infection among Healthy Blood Donors in Northeast of Iran |
title_full_unstemmed |
Prevalence of Toxoplasma gondii Infection among Healthy Blood Donors in Northeast of Iran |
title_sort |
prevalence of toxoplasma gondii infection among healthy blood donors in northeast of iran |
publisher |
Tehran University of Medical Sciences |
series |
Iranian Journal of Parasitology |
issn |
1735-7020 2008-238X |
publishDate |
2017-12-01 |
description |
Background: This cross-sectional investigation aimed to evaluate the prevalence of IgM and IgG anti- Toxoplasma gondii antibodies and the associated risk factors among healthy blood donors in Khorasan Razavi Province, northeast of Iran from Nov 2014 to May 2015.
Methods: Overall, 491 serum samples from apparently healthy blood donors referred the six biggest blood centers in Razavi Khorasan, Iran, were screened for IgG and IgM anti-T. gondii antibodies by enzyme-linked immunosorbent assay (ELISA). A structured questionnaire was used to obtain information on risk factors for T. gondii infection. Nested PCR was also used to detect DNA of T. gondii in the IgM-positive samples by using of B1 and RE (Repetitive Element) as marker for amplifying fragment size of 531 bp and 164 bp in PCR method.
Results: Totally, 200 (40.7%) samples were seropositive for anti-T. gondii antibodies; 184 (37.5%) donors tested seropositive for only IgG antibody, 8 (1.6%) tested seropositive for both IgM and IgG and 8 (1.6%) were positive for IgM antibody alone. Several risk factors significantly related to T. gondii seropositivity in the univariate analysis at P<0.05 included age (P<0.001), and raw/half-cocked meat consumption (P=0.015). T. gondii DNA was found in all sixteen IgM-positive samples.
Conclusion: T. gondii infection was present among healthy blood donors in northeast of Iran. Thus, it is suggested to design screening programs for preventing transfusion-transmitted toxoplasmosis.
|
topic |
Toxoplasmosis Blood transfusion IgG antibody IgM antibody Nested-PCR Iran |
url |
https://ijpa.tums.ac.ir/index.php/ijpa/article/view/1929 |
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