Identification of a Novel (-)-5-Epieremophilene Synthase from Salvia miltiorrhiza via Transcriptome Mining

Identification of a Novel (-)-5-Epieremophilene Synthase from Salvia miltiorrhiza via Transcriptome Mining

Salvia miltiorrhiza, a medicinal plant in China, has been used for thousands of years to treat coronary heart diseases. Although biosynthesis of tanshinones, a group of diterpenoids in S. miltiorrhiza, has been extensively investigated, to date we know little about the formation of monoterpenes and...

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Main Authors: Lei Yang, Xin Fang, Chen-Yi Li, Yue Yang, Meng-Ying Cui, Xiao-Ya Chen
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-04-01
Series:Frontiers in Plant Science
Subjects:
(-)-5-epieremophilene
natural products
Salvia miltiorrhiza
sesquiterpenes
terpene synthases
Online Access:http://journal.frontiersin.org/article/10.3389/fpls.2017.00627/full
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spelling doaj-7dfbfd6978cb4fd0806ddbd8f7a98f572020-11-24T22:40:26ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-04-01810.3389/fpls.2017.00627263420Identification of a Novel (-)-5-Epieremophilene Synthase from Salvia miltiorrhiza via Transcriptome MiningLei Yang0Xin Fang1Xin Fang2Chen-Yi Li3Yue Yang4Meng-Ying Cui5Xiao-Ya Chen6Xiao-Ya Chen7Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden, Shanghai Chenshan Plant Science Research Center, Chinese Academy of SciencesShanghai, ChinaShanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden, Shanghai Chenshan Plant Science Research Center, Chinese Academy of SciencesShanghai, ChinaState Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of SciencesShanghai, ChinaState Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of SciencesShanghai, ChinaShanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden, Shanghai Chenshan Plant Science Research Center, Chinese Academy of SciencesShanghai, ChinaShanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden, Shanghai Chenshan Plant Science Research Center, Chinese Academy of SciencesShanghai, ChinaShanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden, Shanghai Chenshan Plant Science Research Center, Chinese Academy of SciencesShanghai, ChinaState Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of SciencesShanghai, ChinaSalvia miltiorrhiza, a medicinal plant in China, has been used for thousands of years to treat coronary heart diseases. Although biosynthesis of tanshinones, a group of diterpenoids in S. miltiorrhiza, has been extensively investigated, to date we know little about the formation of monoterpenes and sesquiterpenes in this medicinal plant. Here, we report the characterization of three sesquiterpene synthases, named SmSTPS1, SmSTPS2, and SmSTPS3, which catalyzed the formation of a new compound, (-)-5-epieremophilene. Additionally, the (-)-5-epieremophilene biosynthesis activity of SmSTPS1 was confirmed by transient expression in Nicotiana benthamiana. Despite the similar enzyme activities of SmSTPS1, SmSTPS2, and SmSTPS3, the three (-)-5-epieremophilene synthase genes displayed different spatial expression patterns and responded differently to hormone treatments, implicating their specific roles in plant-environment interactions. Our results provide valuable data to understanding the biosynthesis and composition of terpenes in plant.http://journal.frontiersin.org/article/10.3389/fpls.2017.00627/full(-)-5-epieremophilenenatural productsSalvia miltiorrhizasesquiterpenesterpene synthases
collection DOAJ
language English
format Article
sources DOAJ
author Lei Yang
Xin Fang
Xin Fang
Chen-Yi Li
Yue Yang
Meng-Ying Cui
Xiao-Ya Chen
Xiao-Ya Chen
spellingShingle Lei Yang
Xin Fang
Xin Fang
Chen-Yi Li
Yue Yang
Meng-Ying Cui
Xiao-Ya Chen
Xiao-Ya Chen
Identification of a Novel (-)-5-Epieremophilene Synthase from Salvia miltiorrhiza via Transcriptome Mining
Frontiers in Plant Science
(-)-5-epieremophilene
natural products
Salvia miltiorrhiza
sesquiterpenes
terpene synthases
author_facet Lei Yang
Xin Fang
Xin Fang
Chen-Yi Li
Yue Yang
Meng-Ying Cui
Xiao-Ya Chen
Xiao-Ya Chen
author_sort Lei Yang
title Identification of a Novel (-)-5-Epieremophilene Synthase from Salvia miltiorrhiza via Transcriptome Mining
title_short Identification of a Novel (-)-5-Epieremophilene Synthase from Salvia miltiorrhiza via Transcriptome Mining
title_full Identification of a Novel (-)-5-Epieremophilene Synthase from Salvia miltiorrhiza via Transcriptome Mining
title_fullStr Identification of a Novel (-)-5-Epieremophilene Synthase from Salvia miltiorrhiza via Transcriptome Mining
title_full_unstemmed Identification of a Novel (-)-5-Epieremophilene Synthase from Salvia miltiorrhiza via Transcriptome Mining
title_sort identification of a novel (-)-5-epieremophilene synthase from salvia miltiorrhiza via transcriptome mining
publisher Frontiers Media S.A.
series Frontiers in Plant Science
issn 1664-462X
publishDate 2017-04-01
description Salvia miltiorrhiza, a medicinal plant in China, has been used for thousands of years to treat coronary heart diseases. Although biosynthesis of tanshinones, a group of diterpenoids in S. miltiorrhiza, has been extensively investigated, to date we know little about the formation of monoterpenes and sesquiterpenes in this medicinal plant. Here, we report the characterization of three sesquiterpene synthases, named SmSTPS1, SmSTPS2, and SmSTPS3, which catalyzed the formation of a new compound, (-)-5-epieremophilene. Additionally, the (-)-5-epieremophilene biosynthesis activity of SmSTPS1 was confirmed by transient expression in Nicotiana benthamiana. Despite the similar enzyme activities of SmSTPS1, SmSTPS2, and SmSTPS3, the three (-)-5-epieremophilene synthase genes displayed different spatial expression patterns and responded differently to hormone treatments, implicating their specific roles in plant-environment interactions. Our results provide valuable data to understanding the biosynthesis and composition of terpenes in plant.
topic (-)-5-epieremophilene
natural products
Salvia miltiorrhiza
sesquiterpenes
terpene synthases
url http://journal.frontiersin.org/article/10.3389/fpls.2017.00627/full
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