Ethylene Response Factors Are Controlled by Multiple Harvesting Stresses in Hevea brasiliensis.
Tolerance of recurrent mechanical wounding and exogenous ethylene is a feature of the rubber tree. Latex harvesting involves tapping of the tree bark and ethephon is applied to increase latex flow. Ethylene is an essential element in controlling latex production. The ethylene signalling pathway lead...
Main Authors: | , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2015-01-01
|
Series: | PLoS ONE |
Online Access: | https://doi.org/10.1371/journal.pone.0123618 |
id |
doaj-7e039aae8e5e4630b9a7d8640199148e |
---|---|
record_format |
Article |
spelling |
doaj-7e039aae8e5e4630b9a7d8640199148e2021-03-03T20:05:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01104e012361810.1371/journal.pone.0123618Ethylene Response Factors Are Controlled by Multiple Harvesting Stresses in Hevea brasiliensis.Riza-Arief PutrantoCuifang DuanKuswanhadiTetty ChaidamsariMaryannick RioPiyanuch PiyatrakulEva HerlinawatiJulien PirrelloFlorence DessaillyJulie LeclercqFrançois BonnotChaorong TangSongnian HuPascal MontoroTolerance of recurrent mechanical wounding and exogenous ethylene is a feature of the rubber tree. Latex harvesting involves tapping of the tree bark and ethephon is applied to increase latex flow. Ethylene is an essential element in controlling latex production. The ethylene signalling pathway leads to the activation of Ethylene Response Factor (ERF) transcription factors. This family has been identified in Hevea brasiliensis. This study set out to understand the regulation of ERF genes during latex harvesting in relation to abiotic stress and hormonal treatments. Analyses of the relative transcript abundance were carried out for 35 HbERF genes in latex, in bark from mature trees and in leaves from juvenile plants under multiple abiotic stresses. Twenty-one HbERF genes were regulated by harvesting stress in laticifers, revealing an overrepresentation of genes in group IX. Transcripts of three HbERF-IX genes from HbERF-IXc4, HbERF-IXc5 and HbERF-IXc6 were dramatically accumulated by combining wounding, methyl jasmonate and ethylene treatments. When an ethylene inhibitor was used, the transcript accumulation for these three genes was halted, showing ethylene-dependent induction. Subcellular localization and transactivation experiments confirmed that several members of HbERF-IX are activator-type transcription factors. This study suggested that latex harvesting induces mechanisms developed for the response to abiotic stress. These mechanisms probably depend on various hormonal signalling pathways. Several members of HbERF-IX could be essential integrators of complex hormonal signalling pathways in Hevea.https://doi.org/10.1371/journal.pone.0123618 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Riza-Arief Putranto Cuifang Duan Kuswanhadi Tetty Chaidamsari Maryannick Rio Piyanuch Piyatrakul Eva Herlinawati Julien Pirrello Florence Dessailly Julie Leclercq François Bonnot Chaorong Tang Songnian Hu Pascal Montoro |
spellingShingle |
Riza-Arief Putranto Cuifang Duan Kuswanhadi Tetty Chaidamsari Maryannick Rio Piyanuch Piyatrakul Eva Herlinawati Julien Pirrello Florence Dessailly Julie Leclercq François Bonnot Chaorong Tang Songnian Hu Pascal Montoro Ethylene Response Factors Are Controlled by Multiple Harvesting Stresses in Hevea brasiliensis. PLoS ONE |
author_facet |
Riza-Arief Putranto Cuifang Duan Kuswanhadi Tetty Chaidamsari Maryannick Rio Piyanuch Piyatrakul Eva Herlinawati Julien Pirrello Florence Dessailly Julie Leclercq François Bonnot Chaorong Tang Songnian Hu Pascal Montoro |
author_sort |
Riza-Arief Putranto |
title |
Ethylene Response Factors Are Controlled by Multiple Harvesting Stresses in Hevea brasiliensis. |
title_short |
Ethylene Response Factors Are Controlled by Multiple Harvesting Stresses in Hevea brasiliensis. |
title_full |
Ethylene Response Factors Are Controlled by Multiple Harvesting Stresses in Hevea brasiliensis. |
title_fullStr |
Ethylene Response Factors Are Controlled by Multiple Harvesting Stresses in Hevea brasiliensis. |
title_full_unstemmed |
Ethylene Response Factors Are Controlled by Multiple Harvesting Stresses in Hevea brasiliensis. |
title_sort |
ethylene response factors are controlled by multiple harvesting stresses in hevea brasiliensis. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2015-01-01 |
description |
Tolerance of recurrent mechanical wounding and exogenous ethylene is a feature of the rubber tree. Latex harvesting involves tapping of the tree bark and ethephon is applied to increase latex flow. Ethylene is an essential element in controlling latex production. The ethylene signalling pathway leads to the activation of Ethylene Response Factor (ERF) transcription factors. This family has been identified in Hevea brasiliensis. This study set out to understand the regulation of ERF genes during latex harvesting in relation to abiotic stress and hormonal treatments. Analyses of the relative transcript abundance were carried out for 35 HbERF genes in latex, in bark from mature trees and in leaves from juvenile plants under multiple abiotic stresses. Twenty-one HbERF genes were regulated by harvesting stress in laticifers, revealing an overrepresentation of genes in group IX. Transcripts of three HbERF-IX genes from HbERF-IXc4, HbERF-IXc5 and HbERF-IXc6 were dramatically accumulated by combining wounding, methyl jasmonate and ethylene treatments. When an ethylene inhibitor was used, the transcript accumulation for these three genes was halted, showing ethylene-dependent induction. Subcellular localization and transactivation experiments confirmed that several members of HbERF-IX are activator-type transcription factors. This study suggested that latex harvesting induces mechanisms developed for the response to abiotic stress. These mechanisms probably depend on various hormonal signalling pathways. Several members of HbERF-IX could be essential integrators of complex hormonal signalling pathways in Hevea. |
url |
https://doi.org/10.1371/journal.pone.0123618 |
work_keys_str_mv |
AT rizaariefputranto ethyleneresponsefactorsarecontrolledbymultipleharvestingstressesinheveabrasiliensis AT cuifangduan ethyleneresponsefactorsarecontrolledbymultipleharvestingstressesinheveabrasiliensis AT kuswanhadi ethyleneresponsefactorsarecontrolledbymultipleharvestingstressesinheveabrasiliensis AT tettychaidamsari ethyleneresponsefactorsarecontrolledbymultipleharvestingstressesinheveabrasiliensis AT maryannickrio ethyleneresponsefactorsarecontrolledbymultipleharvestingstressesinheveabrasiliensis AT piyanuchpiyatrakul ethyleneresponsefactorsarecontrolledbymultipleharvestingstressesinheveabrasiliensis AT evaherlinawati ethyleneresponsefactorsarecontrolledbymultipleharvestingstressesinheveabrasiliensis AT julienpirrello ethyleneresponsefactorsarecontrolledbymultipleharvestingstressesinheveabrasiliensis AT florencedessailly ethyleneresponsefactorsarecontrolledbymultipleharvestingstressesinheveabrasiliensis AT julieleclercq ethyleneresponsefactorsarecontrolledbymultipleharvestingstressesinheveabrasiliensis AT francoisbonnot ethyleneresponsefactorsarecontrolledbymultipleharvestingstressesinheveabrasiliensis AT chaorongtang ethyleneresponsefactorsarecontrolledbymultipleharvestingstressesinheveabrasiliensis AT songnianhu ethyleneresponsefactorsarecontrolledbymultipleharvestingstressesinheveabrasiliensis AT pascalmontoro ethyleneresponsefactorsarecontrolledbymultipleharvestingstressesinheveabrasiliensis |
_version_ |
1714824095006195712 |