| Summary: | <i>Candida albicans</i> is a commensal polymorphic and opportunistic fungus, which usually resides as a small community in the oral cavities of a majority of humans. The latter eco-system presents this yeast varied opportunities for mutualistic interactions with other cohabitant oral bacteria, that synergizes its persistence and pathogenicity. Collectively, these communities live within complex plaque biofilms which may adversely affect the oral health and increase the proclivity for oral candidiasis. The proteome of such oral biofilms with myriad interkingdom interactions are largely underexplored. Herein, we employed limma differential expression analysis, and cluster analysis to explore the proteomic interactions of <i>C. albicans</i> biofilms with nine different common oral bacterial species, <i>Aggregatibacter actinomycetemcomitans</i>, <i>Actinomyces naeslundii</i>, <i>Fusobacterium nucleatum</i>, <i>Enterococcus faecalis</i>, <i>Porphyromonas gingivalis</i>, <i>Streptococcus mutants</i>, <i>Streptococcus sanguinis</i>, <i>Streptococcus mitis</i>, and <i>Streptococcus sobrinus</i>. Interestingly, upon exposure of <i>C. albicans</i> biofilms to the foregoing heat-killed bacteria, the proteomes of the fungus associated with cellular respiration, translation, oxidoreductase activity, and ligase activity were significantly altered. Subsequent differential expression and cluster analysis revealed the subtle, yet significant alterations in the <i>C. albicans</i> proteome, particularly on exposure to bacteria with dissimilar cell morphologies, and Gram staining characteristics.
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