Immune response induced in mice oral immunization with cowpea severe mosaic virus
There is increasing interest in the immune response induced by plant viruses since these could be used as antigen-expressing systems in vaccination procedures. Cowpea severe mosaic virus (CPSMV), as a purified preparation (300 g of leaves, 2 weeks post-inoculation), or crude extract from cowpea (Vig...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Associação Brasileira de Divulgação Científica
2002-07-01
|
Series: | Brazilian Journal of Medical and Biological Research |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000700011 |
id |
doaj-7e191a239c534ebda175b335b050e805 |
---|---|
record_format |
Article |
spelling |
doaj-7e191a239c534ebda175b335b050e8052020-11-24T22:51:34ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2002-07-0135782783510.1590/S0100-879X2002000700011Immune response induced in mice oral immunization with cowpea severe mosaic virusM.I. FlorindoM.E.F. de AragãoA.C.M. da SilvaM.L. OtochD. Fernandes de MeloJ.A.A. LimaM.G. Silva LimaThere is increasing interest in the immune response induced by plant viruses since these could be used as antigen-expressing systems in vaccination procedures. Cowpea severe mosaic virus (CPSMV), as a purified preparation (300 g of leaves, 2 weeks post-inoculation), or crude extract from cowpea (Vigna unguiculata) leaves infected with CPSMV both administered by gavage to Swiss mice induced a humoral immune response. Groups of 10 Swiss mice (2-month-old females) were immunized orally with 10 daily doses of either 50 µg viral capsid protein (boosters of 50 µg at days 21 and 35 after immunization) or 0.6 mg protein of the crude extract (boosters of 0.6 mg at days 21 and 35 after immunization). Anti-CPSMV antibodies were quantified by ELISA in pooled sera diluted at least 1:400 at days 7, 14, 21, 28, 35 and 42 after the 10th dose. IgG and IgA against CPSMV were produced systemically, but IgE was not detected. No synthesis of specific antibodies against the proteins of leaf extracts from V. unguiculata, infected or not with CPSMV, was detected. The use of CPSMV, a plant-infecting virus that apparently does not induce a pathogenic response in animals, induced a humoral and persistent (at least 6 months) immune response through the administration of low antigen doses by gavage. These results raise the possibility of using CPSMV either as a vector for the production of vaccines against animal pathogens or in quick and easy methods to produce specific antisera for viral diagnosis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000700011Oral immunizationPlant virusPlant virus immunogenicityCowpea severe mosaic virus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
M.I. Florindo M.E.F. de Aragão A.C.M. da Silva M.L. Otoch D. Fernandes de Melo J.A.A. Lima M.G. Silva Lima |
spellingShingle |
M.I. Florindo M.E.F. de Aragão A.C.M. da Silva M.L. Otoch D. Fernandes de Melo J.A.A. Lima M.G. Silva Lima Immune response induced in mice oral immunization with cowpea severe mosaic virus Brazilian Journal of Medical and Biological Research Oral immunization Plant virus Plant virus immunogenicity Cowpea severe mosaic virus |
author_facet |
M.I. Florindo M.E.F. de Aragão A.C.M. da Silva M.L. Otoch D. Fernandes de Melo J.A.A. Lima M.G. Silva Lima |
author_sort |
M.I. Florindo |
title |
Immune response induced in mice oral immunization with cowpea severe mosaic virus |
title_short |
Immune response induced in mice oral immunization with cowpea severe mosaic virus |
title_full |
Immune response induced in mice oral immunization with cowpea severe mosaic virus |
title_fullStr |
Immune response induced in mice oral immunization with cowpea severe mosaic virus |
title_full_unstemmed |
Immune response induced in mice oral immunization with cowpea severe mosaic virus |
title_sort |
immune response induced in mice oral immunization with cowpea severe mosaic virus |
publisher |
Associação Brasileira de Divulgação Científica |
series |
Brazilian Journal of Medical and Biological Research |
issn |
0100-879X 1414-431X |
publishDate |
2002-07-01 |
description |
There is increasing interest in the immune response induced by plant viruses since these could be used as antigen-expressing systems in vaccination procedures. Cowpea severe mosaic virus (CPSMV), as a purified preparation (300 g of leaves, 2 weeks post-inoculation), or crude extract from cowpea (Vigna unguiculata) leaves infected with CPSMV both administered by gavage to Swiss mice induced a humoral immune response. Groups of 10 Swiss mice (2-month-old females) were immunized orally with 10 daily doses of either 50 µg viral capsid protein (boosters of 50 µg at days 21 and 35 after immunization) or 0.6 mg protein of the crude extract (boosters of 0.6 mg at days 21 and 35 after immunization). Anti-CPSMV antibodies were quantified by ELISA in pooled sera diluted at least 1:400 at days 7, 14, 21, 28, 35 and 42 after the 10th dose. IgG and IgA against CPSMV were produced systemically, but IgE was not detected. No synthesis of specific antibodies against the proteins of leaf extracts from V. unguiculata, infected or not with CPSMV, was detected. The use of CPSMV, a plant-infecting virus that apparently does not induce a pathogenic response in animals, induced a humoral and persistent (at least 6 months) immune response through the administration of low antigen doses by gavage. These results raise the possibility of using CPSMV either as a vector for the production of vaccines against animal pathogens or in quick and easy methods to produce specific antisera for viral diagnosis. |
topic |
Oral immunization Plant virus Plant virus immunogenicity Cowpea severe mosaic virus |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000700011 |
work_keys_str_mv |
AT miflorindo immuneresponseinducedinmiceoralimmunizationwithcowpeaseveremosaicvirus AT mefdearagao immuneresponseinducedinmiceoralimmunizationwithcowpeaseveremosaicvirus AT acmdasilva immuneresponseinducedinmiceoralimmunizationwithcowpeaseveremosaicvirus AT mlotoch immuneresponseinducedinmiceoralimmunizationwithcowpeaseveremosaicvirus AT dfernandesdemelo immuneresponseinducedinmiceoralimmunizationwithcowpeaseveremosaicvirus AT jaalima immuneresponseinducedinmiceoralimmunizationwithcowpeaseveremosaicvirus AT mgsilvalima immuneresponseinducedinmiceoralimmunizationwithcowpeaseveremosaicvirus |
_version_ |
1725669045246623744 |