Comprehensive Proteome and Lysine Acetylome Analysis Reveals the Widespread Involvement of Acetylation in Cold Resistance of Pepper (Capsicum annuum L.)
Pepper is a typical warmth-loving vegetable that lacks a cold acclimation mechanism and is sensitive to cold stress. Lysine acetylation plays an important role in diverse cellular processes, but limited knowledge is available regarding acetylation modifications in the resistance of pepper plants to...
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Frontiers Media S.A.
2021-08-01
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Series: | Frontiers in Plant Science |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.730489/full |
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language |
English |
format |
Article |
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DOAJ |
author |
Zhoubin Liu Zhoubin Liu Zhoubin Liu Jingshuang Song Wu Miao Bozhi Yang Bozhi Yang Bozhi Yang Zhuqing Zhang Wenchao Chen Fangjun Tan Huan Suo Huan Suo Huan Suo Xiongze Dai Xiongze Dai Xiongze Dai Xuexiao Zou Xuexiao Zou Xuexiao Zou Lijun Ou Lijun Ou Lijun Ou |
spellingShingle |
Zhoubin Liu Zhoubin Liu Zhoubin Liu Jingshuang Song Wu Miao Bozhi Yang Bozhi Yang Bozhi Yang Zhuqing Zhang Wenchao Chen Fangjun Tan Huan Suo Huan Suo Huan Suo Xiongze Dai Xiongze Dai Xiongze Dai Xuexiao Zou Xuexiao Zou Xuexiao Zou Lijun Ou Lijun Ou Lijun Ou Comprehensive Proteome and Lysine Acetylome Analysis Reveals the Widespread Involvement of Acetylation in Cold Resistance of Pepper (Capsicum annuum L.) Frontiers in Plant Science acetylation modification cold stress carbon fixation pepper proteome photosynthesis |
author_facet |
Zhoubin Liu Zhoubin Liu Zhoubin Liu Jingshuang Song Wu Miao Bozhi Yang Bozhi Yang Bozhi Yang Zhuqing Zhang Wenchao Chen Fangjun Tan Huan Suo Huan Suo Huan Suo Xiongze Dai Xiongze Dai Xiongze Dai Xuexiao Zou Xuexiao Zou Xuexiao Zou Lijun Ou Lijun Ou Lijun Ou |
author_sort |
Zhoubin Liu |
title |
Comprehensive Proteome and Lysine Acetylome Analysis Reveals the Widespread Involvement of Acetylation in Cold Resistance of Pepper (Capsicum annuum L.) |
title_short |
Comprehensive Proteome and Lysine Acetylome Analysis Reveals the Widespread Involvement of Acetylation in Cold Resistance of Pepper (Capsicum annuum L.) |
title_full |
Comprehensive Proteome and Lysine Acetylome Analysis Reveals the Widespread Involvement of Acetylation in Cold Resistance of Pepper (Capsicum annuum L.) |
title_fullStr |
Comprehensive Proteome and Lysine Acetylome Analysis Reveals the Widespread Involvement of Acetylation in Cold Resistance of Pepper (Capsicum annuum L.) |
title_full_unstemmed |
Comprehensive Proteome and Lysine Acetylome Analysis Reveals the Widespread Involvement of Acetylation in Cold Resistance of Pepper (Capsicum annuum L.) |
title_sort |
comprehensive proteome and lysine acetylome analysis reveals the widespread involvement of acetylation in cold resistance of pepper (capsicum annuum l.) |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2021-08-01 |
description |
Pepper is a typical warmth-loving vegetable that lacks a cold acclimation mechanism and is sensitive to cold stress. Lysine acetylation plays an important role in diverse cellular processes, but limited knowledge is available regarding acetylation modifications in the resistance of pepper plants to cold stress. In this study, the proteome and acetylome of two pepper varieties with different levels of cold resistance were investigated by subjecting them to cold treatments of varying durations followed by recovery periods. In total, 6,213 proteins and 4,574 lysine acetylation sites were identified, and this resulted in the discovery of 3,008 differentially expressed proteins and 768 differentially expressed acetylated proteins. A total of 1,988 proteins were identified in both the proteome and acetylome, and the functional differences in these co-identified proteins were elucidated through GO enrichment. KEGG analysis showed that 397 identified acetylated proteins were involved in 93 different metabolic pathways. The dynamic changes in the acetylated proteins in photosynthesis and the “carbon fixation in the photosynthetic organisms” pathway in pepper under low-temperature stress were further analyzed. It was found that acetylation of the PsbO and PsbR proteins in photosystem II and the PsaN protein in photosystem I could regulate the response of pepper leaves to cold stress. The acetylation levels of key carbon assimilation enzymes, such as ribulose bisphosphate carboxylase, fructose-1,6-bisphosphatase, sedoheptulose-1,7-bisphosphatase, glyceraldehyde 3-phosphate dehydrogenase, phosphoribulokinase, and triosephosphate isomerase decreased, leading to decreases in carbon assimilation capacity and photosynthetic efficiency, reducing the cold tolerance of pepper leaves. This study is the first to identify the acetylome in pepper, and it greatly expands the catalog of lysine acetylation substrates and sites in Solanaceae crops, providing new insights for posttranslational modification studies. |
topic |
acetylation modification cold stress carbon fixation pepper proteome photosynthesis |
url |
https://www.frontiersin.org/articles/10.3389/fpls.2021.730489/full |
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doaj-7e4aa50fcca14033b24a978dedc009782021-09-03T08:47:21ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-08-011210.3389/fpls.2021.730489730489Comprehensive Proteome and Lysine Acetylome Analysis Reveals the Widespread Involvement of Acetylation in Cold Resistance of Pepper (Capsicum annuum L.)Zhoubin Liu0Zhoubin Liu1Zhoubin Liu2Jingshuang Song3Wu Miao4Bozhi Yang5Bozhi Yang6Bozhi Yang7Zhuqing Zhang8Wenchao Chen9Fangjun Tan10Huan Suo11Huan Suo12Huan Suo13Xiongze Dai14Xiongze Dai15Xiongze Dai16Xuexiao Zou17Xuexiao Zou18Xuexiao Zou19Lijun Ou20Lijun Ou21Lijun Ou22College of Horticulture, Hunan Agricultural University, Changsha, ChinaERC for Germplasm Innovation and New Variety Breeding of Horticultural Crops, Changsha, ChinaKey Laboratory for Vegetable Biology of Hunan Province, Changsha, ChinaVegetable Research Institute, Hunan Academy of Agricultural Science, Changsha, ChinaHunan Xiangyan Seed Industry Co., Ltd, Changsha, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha, ChinaERC for Germplasm Innovation and New Variety Breeding of Horticultural Crops, Changsha, ChinaKey Laboratory for Vegetable Biology of Hunan Province, Changsha, ChinaVegetable Research Institute, Hunan Academy of Agricultural Science, Changsha, ChinaVegetable Research Institute, Hunan Academy of Agricultural Science, Changsha, ChinaVegetable Research Institute, Hunan Academy of Agricultural Science, Changsha, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha, ChinaERC for Germplasm Innovation and New Variety Breeding of Horticultural Crops, Changsha, ChinaKey Laboratory for Vegetable Biology of Hunan Province, Changsha, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha, ChinaERC for Germplasm Innovation and New Variety Breeding of Horticultural Crops, Changsha, ChinaKey Laboratory for Vegetable Biology of Hunan Province, Changsha, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha, ChinaERC for Germplasm Innovation and New Variety Breeding of Horticultural Crops, Changsha, ChinaKey Laboratory for Vegetable Biology of Hunan Province, Changsha, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha, ChinaERC for Germplasm Innovation and New Variety Breeding of Horticultural Crops, Changsha, ChinaKey Laboratory for Vegetable Biology of Hunan Province, Changsha, ChinaPepper is a typical warmth-loving vegetable that lacks a cold acclimation mechanism and is sensitive to cold stress. Lysine acetylation plays an important role in diverse cellular processes, but limited knowledge is available regarding acetylation modifications in the resistance of pepper plants to cold stress. In this study, the proteome and acetylome of two pepper varieties with different levels of cold resistance were investigated by subjecting them to cold treatments of varying durations followed by recovery periods. In total, 6,213 proteins and 4,574 lysine acetylation sites were identified, and this resulted in the discovery of 3,008 differentially expressed proteins and 768 differentially expressed acetylated proteins. A total of 1,988 proteins were identified in both the proteome and acetylome, and the functional differences in these co-identified proteins were elucidated through GO enrichment. KEGG analysis showed that 397 identified acetylated proteins were involved in 93 different metabolic pathways. The dynamic changes in the acetylated proteins in photosynthesis and the “carbon fixation in the photosynthetic organisms” pathway in pepper under low-temperature stress were further analyzed. It was found that acetylation of the PsbO and PsbR proteins in photosystem II and the PsaN protein in photosystem I could regulate the response of pepper leaves to cold stress. The acetylation levels of key carbon assimilation enzymes, such as ribulose bisphosphate carboxylase, fructose-1,6-bisphosphatase, sedoheptulose-1,7-bisphosphatase, glyceraldehyde 3-phosphate dehydrogenase, phosphoribulokinase, and triosephosphate isomerase decreased, leading to decreases in carbon assimilation capacity and photosynthetic efficiency, reducing the cold tolerance of pepper leaves. This study is the first to identify the acetylome in pepper, and it greatly expands the catalog of lysine acetylation substrates and sites in Solanaceae crops, providing new insights for posttranslational modification studies.https://www.frontiersin.org/articles/10.3389/fpls.2021.730489/fullacetylation modificationcold stresscarbon fixationpepperproteomephotosynthesis |