The synergistic interference effect of silica nanoparticles concentration and the wavelength of ELISA on the colorimetric assay of cell toxicity
Abstract The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay is the most common method for the determination of cell toxicity, but some factors limit the sensitivity of this method, such as pH. Less attention had been paid to the interference effect of optical and plasmonic...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2021-07-01
|
Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-021-92419-1 |
id |
doaj-7e5e823b6cca45aba4e73a7d04b7e8bc |
---|---|
record_format |
Article |
spelling |
doaj-7e5e823b6cca45aba4e73a7d04b7e8bc2021-07-25T11:26:29ZengNature Publishing GroupScientific Reports2045-23222021-07-0111111110.1038/s41598-021-92419-1The synergistic interference effect of silica nanoparticles concentration and the wavelength of ELISA on the colorimetric assay of cell toxicityFariba Abbasi0Hassan Hashemi1Mohammad Reza Samaei2Amir SavarDashtaki3Abooalfazl Azhdarpoor4Mohammad Javad Fallahi5Department of Environmental Health Engineering, School of Health, Shiraz University of Medical SciencesDepartment of Environmental Health Engineering, School of Health, Shiraz University of Medical SciencesDepartment of Environmental Health Engineering, School of Health, Shiraz University of Medical SciencesDepartment of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical SciencesDepartment of Environmental Health Engineering, School of Health, Shiraz University of Medical SciencesDepartment of Internal Medicine, School of Medicine, Shiraz University of Medical SciencesAbstract The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay is the most common method for the determination of cell toxicity, but some factors limit the sensitivity of this method, such as pH. Less attention had been paid to the interference effect of optical and plasmonic properties of SiO2 nanoparticles (NPs) in the wavelength range assigned to MTT. This study investigated the synergistic interference effect of SiO2 NPs and wavelength on MTT assay for the first time. The examined variables included the type of SiO2 NPs concentrations (1, 10, and 100 mM) and different wavelengths (470, 490, 520, and 570 nm). The results showed that optical density (OD) increased (p < 0.05) when wavelength and the concentration of crystalline SiO2 NPs increased. So, the maximum OD at 10 and 100 mM were attributed to crystalline SiO2 NPs (p < 0.05) due to the functional group, whereas it was related to amorphous at 1 mM (p > 0.05). According to polynomial regression modeling (PRM), the maximum interference effect was predicted at crystalline SiO2 NPs and wavelength > 550 nm. Besides, the synergistic effects of SiO2 NPs, wavelength, and concentration of NPs had been a good fitting with first-order PRM. Thus, the concentration of SiO2 NPs had a confounder factor in colorimetric for MTT assay. The best artificial neural network (ANN) structure was related to the 3:7:1 network (Rall = 0.936, MSE = 0.0006, MAPE = 0.063). The correlation between the actual and predicted data was 0.88. As SiO2 NPs presence is an interfering factor in MTT assay concerning wavelength, it is suggested wavelength use with minimum confounding effect for MTT assay.https://doi.org/10.1038/s41598-021-92419-1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fariba Abbasi Hassan Hashemi Mohammad Reza Samaei Amir SavarDashtaki Abooalfazl Azhdarpoor Mohammad Javad Fallahi |
spellingShingle |
Fariba Abbasi Hassan Hashemi Mohammad Reza Samaei Amir SavarDashtaki Abooalfazl Azhdarpoor Mohammad Javad Fallahi The synergistic interference effect of silica nanoparticles concentration and the wavelength of ELISA on the colorimetric assay of cell toxicity Scientific Reports |
author_facet |
Fariba Abbasi Hassan Hashemi Mohammad Reza Samaei Amir SavarDashtaki Abooalfazl Azhdarpoor Mohammad Javad Fallahi |
author_sort |
Fariba Abbasi |
title |
The synergistic interference effect of silica nanoparticles concentration and the wavelength of ELISA on the colorimetric assay of cell toxicity |
title_short |
The synergistic interference effect of silica nanoparticles concentration and the wavelength of ELISA on the colorimetric assay of cell toxicity |
title_full |
The synergistic interference effect of silica nanoparticles concentration and the wavelength of ELISA on the colorimetric assay of cell toxicity |
title_fullStr |
The synergistic interference effect of silica nanoparticles concentration and the wavelength of ELISA on the colorimetric assay of cell toxicity |
title_full_unstemmed |
The synergistic interference effect of silica nanoparticles concentration and the wavelength of ELISA on the colorimetric assay of cell toxicity |
title_sort |
synergistic interference effect of silica nanoparticles concentration and the wavelength of elisa on the colorimetric assay of cell toxicity |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-07-01 |
description |
Abstract The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay is the most common method for the determination of cell toxicity, but some factors limit the sensitivity of this method, such as pH. Less attention had been paid to the interference effect of optical and plasmonic properties of SiO2 nanoparticles (NPs) in the wavelength range assigned to MTT. This study investigated the synergistic interference effect of SiO2 NPs and wavelength on MTT assay for the first time. The examined variables included the type of SiO2 NPs concentrations (1, 10, and 100 mM) and different wavelengths (470, 490, 520, and 570 nm). The results showed that optical density (OD) increased (p < 0.05) when wavelength and the concentration of crystalline SiO2 NPs increased. So, the maximum OD at 10 and 100 mM were attributed to crystalline SiO2 NPs (p < 0.05) due to the functional group, whereas it was related to amorphous at 1 mM (p > 0.05). According to polynomial regression modeling (PRM), the maximum interference effect was predicted at crystalline SiO2 NPs and wavelength > 550 nm. Besides, the synergistic effects of SiO2 NPs, wavelength, and concentration of NPs had been a good fitting with first-order PRM. Thus, the concentration of SiO2 NPs had a confounder factor in colorimetric for MTT assay. The best artificial neural network (ANN) structure was related to the 3:7:1 network (Rall = 0.936, MSE = 0.0006, MAPE = 0.063). The correlation between the actual and predicted data was 0.88. As SiO2 NPs presence is an interfering factor in MTT assay concerning wavelength, it is suggested wavelength use with minimum confounding effect for MTT assay. |
url |
https://doi.org/10.1038/s41598-021-92419-1 |
work_keys_str_mv |
AT faribaabbasi thesynergisticinterferenceeffectofsilicananoparticlesconcentrationandthewavelengthofelisaonthecolorimetricassayofcelltoxicity AT hassanhashemi thesynergisticinterferenceeffectofsilicananoparticlesconcentrationandthewavelengthofelisaonthecolorimetricassayofcelltoxicity AT mohammadrezasamaei thesynergisticinterferenceeffectofsilicananoparticlesconcentrationandthewavelengthofelisaonthecolorimetricassayofcelltoxicity AT amirsavardashtaki thesynergisticinterferenceeffectofsilicananoparticlesconcentrationandthewavelengthofelisaonthecolorimetricassayofcelltoxicity AT abooalfazlazhdarpoor thesynergisticinterferenceeffectofsilicananoparticlesconcentrationandthewavelengthofelisaonthecolorimetricassayofcelltoxicity AT mohammadjavadfallahi thesynergisticinterferenceeffectofsilicananoparticlesconcentrationandthewavelengthofelisaonthecolorimetricassayofcelltoxicity AT faribaabbasi synergisticinterferenceeffectofsilicananoparticlesconcentrationandthewavelengthofelisaonthecolorimetricassayofcelltoxicity AT hassanhashemi synergisticinterferenceeffectofsilicananoparticlesconcentrationandthewavelengthofelisaonthecolorimetricassayofcelltoxicity AT mohammadrezasamaei synergisticinterferenceeffectofsilicananoparticlesconcentrationandthewavelengthofelisaonthecolorimetricassayofcelltoxicity AT amirsavardashtaki synergisticinterferenceeffectofsilicananoparticlesconcentrationandthewavelengthofelisaonthecolorimetricassayofcelltoxicity AT abooalfazlazhdarpoor synergisticinterferenceeffectofsilicananoparticlesconcentrationandthewavelengthofelisaonthecolorimetricassayofcelltoxicity AT mohammadjavadfallahi synergisticinterferenceeffectofsilicananoparticlesconcentrationandthewavelengthofelisaonthecolorimetricassayofcelltoxicity |
_version_ |
1721283193767723008 |