Effect of metabolic control on the in vitro proliferation of peripheral blood mononuclear cells in type 1 and type 2 diabetic patients
CONTEXT AND OBJECTIVE: Diabetes mellitus is a clinical syndrome that frequently leads to the development of chronic complications and high susceptibility to infections. It is probably due to defective immunological defense, which may be related to metabolic control of the disease. The aim of this st...
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doaj-7e6e2c3199e04f3c9be28245f7d9ecf32020-11-25T01:10:26ZengAssociação Paulista de MedicinaSão Paulo Medical Journal1806-9460124421922210.1590/S1516-31802006000400009S1516-31802006000400009Effect of metabolic control on the in vitro proliferation of peripheral blood mononuclear cells in type 1 and type 2 diabetic patientsMaria Cristina Foss-Freitas0Norma Tiraboschi Foss1Eduardo Antonio Donadi2Milton Cesar Foss3Universidade de São PauloUniversidade de São PauloUniversidade de São PauloUniversidade de São PauloCONTEXT AND OBJECTIVE: Diabetes mellitus is a clinical syndrome that frequently leads to the development of chronic complications and high susceptibility to infections. It is probably due to defective immunological defense, which may be related to metabolic control of the disease. The aim of this study was to evaluate the effect of metabolic control on immune-cell behavior in type 1 and type 2 diabetic patients. For this, the in vitro proliferation of peripheral blood mononuclear cells (PBMC) was analyzed in patients with inadequate and adequate metabolic control. DESIGN AND SETTING: Experimental/laboratory study at a university hospital. METHODS: Eleven type 1 and thirteen type 2 diabetic patients were studied, together with 21 healthy individuals divided in two groups (11/10), who were matched by sex and age with those diabetic patients. PBMC cultures stimulated with concanavalin-A (Con-A) were used to measure ³H-thymidine incorporation after 72 hours of cell culturing. For patients with inadequate metabolic control, culturing was performed on the first day of patient hospitalization and again after intensive treatment to achieve adequate control. RESULTS: The proliferation index for Con-A-stimulated cultures from type 1 diabetic patients was significantly greater than that for cultures from healthy individuals and type 2 diabetic patients, independent of metabolic control. A negative correlation between the proliferation cell index and body mass index and serum C-reactive protein levels was also observed. CONCLUSION: The increase in the proliferation capacity of type 1 diabetic T lymphocytes was probably not caused by hyperglycemia and/or insulinopenia related to inadequate metabolic control.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802006000400009&lng=en&tlng=enCell cultureLymphocyte transformationType I diabetes mellitusType II diabetes mellitusHyperglycemiaInfection |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maria Cristina Foss-Freitas Norma Tiraboschi Foss Eduardo Antonio Donadi Milton Cesar Foss |
spellingShingle |
Maria Cristina Foss-Freitas Norma Tiraboschi Foss Eduardo Antonio Donadi Milton Cesar Foss Effect of metabolic control on the in vitro proliferation of peripheral blood mononuclear cells in type 1 and type 2 diabetic patients São Paulo Medical Journal Cell culture Lymphocyte transformation Type I diabetes mellitus Type II diabetes mellitus Hyperglycemia Infection |
author_facet |
Maria Cristina Foss-Freitas Norma Tiraboschi Foss Eduardo Antonio Donadi Milton Cesar Foss |
author_sort |
Maria Cristina Foss-Freitas |
title |
Effect of metabolic control on the in vitro proliferation of peripheral blood mononuclear cells in type 1 and type 2 diabetic patients |
title_short |
Effect of metabolic control on the in vitro proliferation of peripheral blood mononuclear cells in type 1 and type 2 diabetic patients |
title_full |
Effect of metabolic control on the in vitro proliferation of peripheral blood mononuclear cells in type 1 and type 2 diabetic patients |
title_fullStr |
Effect of metabolic control on the in vitro proliferation of peripheral blood mononuclear cells in type 1 and type 2 diabetic patients |
title_full_unstemmed |
Effect of metabolic control on the in vitro proliferation of peripheral blood mononuclear cells in type 1 and type 2 diabetic patients |
title_sort |
effect of metabolic control on the in vitro proliferation of peripheral blood mononuclear cells in type 1 and type 2 diabetic patients |
publisher |
Associação Paulista de Medicina |
series |
São Paulo Medical Journal |
issn |
1806-9460 |
description |
CONTEXT AND OBJECTIVE: Diabetes mellitus is a clinical syndrome that frequently leads to the development of chronic complications and high susceptibility to infections. It is probably due to defective immunological defense, which may be related to metabolic control of the disease. The aim of this study was to evaluate the effect of metabolic control on immune-cell behavior in type 1 and type 2 diabetic patients. For this, the in vitro proliferation of peripheral blood mononuclear cells (PBMC) was analyzed in patients with inadequate and adequate metabolic control. DESIGN AND SETTING: Experimental/laboratory study at a university hospital. METHODS: Eleven type 1 and thirteen type 2 diabetic patients were studied, together with 21 healthy individuals divided in two groups (11/10), who were matched by sex and age with those diabetic patients. PBMC cultures stimulated with concanavalin-A (Con-A) were used to measure ³H-thymidine incorporation after 72 hours of cell culturing. For patients with inadequate metabolic control, culturing was performed on the first day of patient hospitalization and again after intensive treatment to achieve adequate control. RESULTS: The proliferation index for Con-A-stimulated cultures from type 1 diabetic patients was significantly greater than that for cultures from healthy individuals and type 2 diabetic patients, independent of metabolic control. A negative correlation between the proliferation cell index and body mass index and serum C-reactive protein levels was also observed. CONCLUSION: The increase in the proliferation capacity of type 1 diabetic T lymphocytes was probably not caused by hyperglycemia and/or insulinopenia related to inadequate metabolic control. |
topic |
Cell culture Lymphocyte transformation Type I diabetes mellitus Type II diabetes mellitus Hyperglycemia Infection |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802006000400009&lng=en&tlng=en |
work_keys_str_mv |
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