Antioxidant Activity and Phytochemical Composition of the Leaves of Solanum guaraniticum A. St.-Hil
Solanum guaraniticum is a shrub belonging to the Solanaceae family popularly known in Brazil as jurubeba or false-jurubeba. The aim of this study was to evaluate the antioxidant activity of crude extract and chloroform, ethyl acetate and n-butanol fractions from its leaves, verifying the ability to...
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doaj-7e924e269f0148d59b869a5afe1374032020-11-24T23:02:34ZengMDPI AGMolecules1420-30492012-10-011711125601257410.3390/molecules171112560Antioxidant Activity and Phytochemical Composition of the Leaves of Solanum guaraniticum A. St.-HilFélix Alexandre Antunes SoaresMargareth Linde AthaydeSílvio Terra StefanelloMichel Mansur MachadoRobson Borba de FreitasAline Augusti BoligonThiele Faccim de BrumMariana PianaMarina ZadraSolanum guaraniticum is a shrub belonging to the Solanaceae family popularly known in Brazil as jurubeba or false-jurubeba. The aim of this study was to evaluate the antioxidant activity of crude extract and chloroform, ethyl acetate and n-butanol fractions from its leaves, verifying the ability to remove reactive species and identify and quantify phenolic compounds. The ethyl acetate fraction showed the highest amount of total polyphenols (546.57 ± 2.35 mg gallic acid equivalent/g) and the lowest IC50 (9.11 ± 0.75 µg/mL) by the DPPH method. Furthermore, the chloroform fraction presented the highest content of flavonoids (75.73 ± 0.34 mg rutin equivalents/g), tannins (56.03 ± 0.68 mg catechin equivalents/g) and alkaloids (10.79 ± 0.06 mg/g). This fraction was effective in the scavenging of reactive species by 2′,7′-dichlorofluorescein diacetate assay, in addition to completely reducing protein carbonyl content and reducing lipid peroxidation at basal levels even at low concentrations. Chlorogenic, caffeic and rosmarinic acids were identified and quantified by HPLC/DAD. These results show that S. guaraniticum is rich in phenolic compounds and has potential as an antioxidant.http://www.mdpi.com/1420-3049/17/11/12560Solanum guaraniticumantioxidant activitypolyphenolsHPLC/DAD |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Félix Alexandre Antunes Soares Margareth Linde Athayde Sílvio Terra Stefanello Michel Mansur Machado Robson Borba de Freitas Aline Augusti Boligon Thiele Faccim de Brum Mariana Piana Marina Zadra |
spellingShingle |
Félix Alexandre Antunes Soares Margareth Linde Athayde Sílvio Terra Stefanello Michel Mansur Machado Robson Borba de Freitas Aline Augusti Boligon Thiele Faccim de Brum Mariana Piana Marina Zadra Antioxidant Activity and Phytochemical Composition of the Leaves of Solanum guaraniticum A. St.-Hil Molecules Solanum guaraniticum antioxidant activity polyphenols HPLC/DAD |
author_facet |
Félix Alexandre Antunes Soares Margareth Linde Athayde Sílvio Terra Stefanello Michel Mansur Machado Robson Borba de Freitas Aline Augusti Boligon Thiele Faccim de Brum Mariana Piana Marina Zadra |
author_sort |
Félix Alexandre Antunes Soares |
title |
Antioxidant Activity and Phytochemical Composition of the Leaves of Solanum guaraniticum A. St.-Hil |
title_short |
Antioxidant Activity and Phytochemical Composition of the Leaves of Solanum guaraniticum A. St.-Hil |
title_full |
Antioxidant Activity and Phytochemical Composition of the Leaves of Solanum guaraniticum A. St.-Hil |
title_fullStr |
Antioxidant Activity and Phytochemical Composition of the Leaves of Solanum guaraniticum A. St.-Hil |
title_full_unstemmed |
Antioxidant Activity and Phytochemical Composition of the Leaves of Solanum guaraniticum A. St.-Hil |
title_sort |
antioxidant activity and phytochemical composition of the leaves of solanum guaraniticum a. st.-hil |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2012-10-01 |
description |
Solanum guaraniticum is a shrub belonging to the Solanaceae family popularly known in Brazil as jurubeba or false-jurubeba. The aim of this study was to evaluate the antioxidant activity of crude extract and chloroform, ethyl acetate and n-butanol fractions from its leaves, verifying the ability to remove reactive species and identify and quantify phenolic compounds. The ethyl acetate fraction showed the highest amount of total polyphenols (546.57 ± 2.35 mg gallic acid equivalent/g) and the lowest IC50 (9.11 ± 0.75 µg/mL) by the DPPH method. Furthermore, the chloroform fraction presented the highest content of flavonoids (75.73 ± 0.34 mg rutin equivalents/g), tannins (56.03 ± 0.68 mg catechin equivalents/g) and alkaloids (10.79 ± 0.06 mg/g). This fraction was effective in the scavenging of reactive species by 2′,7′-dichlorofluorescein diacetate assay, in addition to completely reducing protein carbonyl content and reducing lipid peroxidation at basal levels even at low concentrations. Chlorogenic, caffeic and rosmarinic acids were identified and quantified by HPLC/DAD. These results show that S. guaraniticum is rich in phenolic compounds and has potential as an antioxidant. |
topic |
Solanum guaraniticum antioxidant activity polyphenols HPLC/DAD |
url |
http://www.mdpi.com/1420-3049/17/11/12560 |
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