High-performance capillary electrophoresis for determining HIV-1 Tat protein in neurons.
The HIV-1 protein, Tat has been implicated in AIDS pathogenesis however, the amount of circulating Tat is believed to be very low and its quantification has been difficult. We performed the quantification of Tat released from infected cells and taken up by neurons using high performance capillary el...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2011-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3017553?pdf=render |
id |
doaj-7ec3c9a05e8f4d30836a2b83a1758d2f |
---|---|
record_format |
Article |
spelling |
doaj-7ec3c9a05e8f4d30836a2b83a1758d2f2020-11-24T21:23:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0161e1614810.1371/journal.pone.0016148High-performance capillary electrophoresis for determining HIV-1 Tat protein in neurons.Satish L DeshmaneRuma MukerjeeShongshan FanBassel E SawayaThe HIV-1 protein, Tat has been implicated in AIDS pathogenesis however, the amount of circulating Tat is believed to be very low and its quantification has been difficult. We performed the quantification of Tat released from infected cells and taken up by neurons using high performance capillary electrophoresis. This is the first report to successfully measure the amount of Tat in neurons and places Tat as a key player involved in HIV-associated neurocognitive disorders.http://europepmc.org/articles/PMC3017553?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Satish L Deshmane Ruma Mukerjee Shongshan Fan Bassel E Sawaya |
spellingShingle |
Satish L Deshmane Ruma Mukerjee Shongshan Fan Bassel E Sawaya High-performance capillary electrophoresis for determining HIV-1 Tat protein in neurons. PLoS ONE |
author_facet |
Satish L Deshmane Ruma Mukerjee Shongshan Fan Bassel E Sawaya |
author_sort |
Satish L Deshmane |
title |
High-performance capillary electrophoresis for determining HIV-1 Tat protein in neurons. |
title_short |
High-performance capillary electrophoresis for determining HIV-1 Tat protein in neurons. |
title_full |
High-performance capillary electrophoresis for determining HIV-1 Tat protein in neurons. |
title_fullStr |
High-performance capillary electrophoresis for determining HIV-1 Tat protein in neurons. |
title_full_unstemmed |
High-performance capillary electrophoresis for determining HIV-1 Tat protein in neurons. |
title_sort |
high-performance capillary electrophoresis for determining hiv-1 tat protein in neurons. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2011-01-01 |
description |
The HIV-1 protein, Tat has been implicated in AIDS pathogenesis however, the amount of circulating Tat is believed to be very low and its quantification has been difficult. We performed the quantification of Tat released from infected cells and taken up by neurons using high performance capillary electrophoresis. This is the first report to successfully measure the amount of Tat in neurons and places Tat as a key player involved in HIV-associated neurocognitive disorders. |
url |
http://europepmc.org/articles/PMC3017553?pdf=render |
work_keys_str_mv |
AT satishldeshmane highperformancecapillaryelectrophoresisfordetermininghiv1tatproteininneurons AT rumamukerjee highperformancecapillaryelectrophoresisfordetermininghiv1tatproteininneurons AT shongshanfan highperformancecapillaryelectrophoresisfordetermininghiv1tatproteininneurons AT basselesawaya highperformancecapillaryelectrophoresisfordetermininghiv1tatproteininneurons |
_version_ |
1725992498857246720 |