Determination of extra and intracellular content from some lytic enzymes related with carnation (Dianthus caryophyllus L.) root cell wall
The presence of some enzymes related to cell wall (polygalacturonase, the pectate lyase, protease and xylanase) in carnation (Dianthus caryophyllus L.) roots as well as the activity levels were determined. These levels were analyzed in different cellular places: the intercellular fluid that is part...
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Universidad Nacional de Colombia
2016-11-01
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doaj-7ed52603547141e38e7d6da2e5aa8bf02020-11-24T23:28:18ZengUniversidad Nacional de ColombiaRevista Colombiana de Química0120-28042357-37912016-11-0145251110.15446/rev.colomb.quim.v45n2.6037043953Determination of extra and intracellular content from some lytic enzymes related with carnation (Dianthus caryophyllus L.) root cell wallSixta Tulia Martínez Peralta0Edilene Ramínez Vargas1Universidad Nacional de ColombiaUniversidad Nacional de ColombiaThe presence of some enzymes related to cell wall (polygalacturonase, the pectate lyase, protease and xylanase) in carnation (Dianthus caryophyllus L.) roots as well as the activity levels were determined. These levels were analyzed in different cellular places: the intercellular fluid that is part of apoplast, the symplast, and the total level (apoplast and symplast) in carnation roots. Two methods were tested to extract the intercellular fluid. To obtain the intracellular content (symplast) and total extract (apoplast+symplast), three methods were tested, using as extracting solution i) phosphate buffer, ii) phosphate buffer + PVPP, iii) before the extraction with phosphate buffer, the carnation roots were washed with acetone. The results showed the effect of different extracting solutions in the enzymatic activities and in the protein content. A new only one step method is proposed to extract the four enzymes and make the comparative analysis of enzymatic activity.http://www.revistas.unal.edu.co/index.php/rcolquim/article/view/60370fluido intercelularextracto intracelularactividad enzimáticapoligalacturonasapectato liasaproteasaxilanasa |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sixta Tulia Martínez Peralta Edilene Ramínez Vargas |
spellingShingle |
Sixta Tulia Martínez Peralta Edilene Ramínez Vargas Determination of extra and intracellular content from some lytic enzymes related with carnation (Dianthus caryophyllus L.) root cell wall Revista Colombiana de Química fluido intercelular extracto intracelular actividad enzimática poligalacturonasa pectato liasa proteasa xilanasa |
author_facet |
Sixta Tulia Martínez Peralta Edilene Ramínez Vargas |
author_sort |
Sixta Tulia Martínez Peralta |
title |
Determination of extra and intracellular content from some lytic enzymes related with carnation (Dianthus caryophyllus L.) root cell wall |
title_short |
Determination of extra and intracellular content from some lytic enzymes related with carnation (Dianthus caryophyllus L.) root cell wall |
title_full |
Determination of extra and intracellular content from some lytic enzymes related with carnation (Dianthus caryophyllus L.) root cell wall |
title_fullStr |
Determination of extra and intracellular content from some lytic enzymes related with carnation (Dianthus caryophyllus L.) root cell wall |
title_full_unstemmed |
Determination of extra and intracellular content from some lytic enzymes related with carnation (Dianthus caryophyllus L.) root cell wall |
title_sort |
determination of extra and intracellular content from some lytic enzymes related with carnation (dianthus caryophyllus l.) root cell wall |
publisher |
Universidad Nacional de Colombia |
series |
Revista Colombiana de Química |
issn |
0120-2804 2357-3791 |
publishDate |
2016-11-01 |
description |
The presence of some enzymes related to cell wall (polygalacturonase, the pectate lyase, protease and xylanase) in carnation (Dianthus caryophyllus L.) roots as well as the activity levels were determined. These levels were analyzed in different cellular places: the intercellular fluid that is part of apoplast, the symplast, and the total level (apoplast and symplast) in carnation roots. Two methods were tested to extract the intercellular fluid. To obtain the intracellular content (symplast) and total extract (apoplast+symplast), three methods were tested, using as extracting solution i) phosphate buffer, ii) phosphate buffer + PVPP, iii) before the extraction with phosphate buffer, the carnation roots were washed with acetone. The results showed the effect of different extracting solutions in the enzymatic activities and in the protein content. A new only one step method is proposed to extract the four enzymes and make the comparative analysis of enzymatic activity. |
topic |
fluido intercelular extracto intracelular actividad enzimática poligalacturonasa pectato liasa proteasa xilanasa |
url |
http://www.revistas.unal.edu.co/index.php/rcolquim/article/view/60370 |
work_keys_str_mv |
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