Identification of Echinococcus granulosus Strains in Isolated Hydatid Cyst Specimens from Animals by PCR-RFLP Method in West Azerbaijan - Iran
Background: The aim of this study was DNA extraction from protosco-lecses of Echinococcus granulosus and identification of these strains in West- Azerbaijan Province, north western Iran. Methods: Thirty one livestock isolates from sheep and cattle were collected from abattoirs of the provinc...
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doaj-7ed552d5b2b44c9ab67231a1af48e7b42021-03-02T09:01:47ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2013-09-0183Identification of Echinococcus granulosus Strains in Isolated Hydatid Cyst Specimens from Animals by PCR-RFLP Method in West Azerbaijan - IranHaleh Hanifian0Kambiz Diba1Khosrow Hazrati Tappeh2Habib Mohammadzadeh3Rahim Mahmoudlou4Dept. of Medical Parasitology and Mycology, Faculty of medicine, Urmia University of Medical Sciences, Urmia, Iran.Dept. of Medical Parasitology and Mycology, Faculty of medicine, Urmia University of Medical Sciences, Urmia, Iran.Cellular and Molecular Research center, Urmia University of Medical Seiences, Urmia-Iran.Dept. of Medical Parasitology and Mycology, Faculty of medicine, Urmia University of Medical Sciences, Urmia, Iran.Dept. of surgery, Faculty of medicine, Urmia University of Medical Sciences, Urmia-Iran. Background: The aim of this study was DNA extraction from protosco-lecses of Echinococcus granulosus and identification of these strains in West- Azerbaijan Province, north western Iran. Methods: Thirty one livestock isolates from sheep and cattle were collected from abattoirs of the province. To investigate the genetic variation of the isolates, after DNA extraction by Glass beads-phenol chloroform method; PCR-RLFP analysis of rDNA-ITS1 was performed using three different re-striction enzymes of Taq 1, Rsa 1 and Alu 1. Result: Amplified PCR products for all isolates were 1000bp band which is expected band in sheep strains (G1-G3 complex). The results of RFLP analy-sis also were the same for all isolates. PCR-RFLP patterns restriction en-zymes were identical as follows, Rsa1 bands under UV showed two bands approximately 655bp and 345bp. Alu1 bands were as follows: two approx-imately 800bp and 200bp and Taq1 did not cut any region and bands were approximately 1000 bp in all samples. Conclusions: Based on PCR-RFLP patterns of ITS1 fragment produced with endonucleases enzyme digestion in animal isolates, it can be concluded that a single strain of E. granulosus (sheep strain or G1-G3 complex) is domi-nant genotype in this province. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/481Hydatid cystITS1RFLP-PCRStrain |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Haleh Hanifian Kambiz Diba Khosrow Hazrati Tappeh Habib Mohammadzadeh Rahim Mahmoudlou |
spellingShingle |
Haleh Hanifian Kambiz Diba Khosrow Hazrati Tappeh Habib Mohammadzadeh Rahim Mahmoudlou Identification of Echinococcus granulosus Strains in Isolated Hydatid Cyst Specimens from Animals by PCR-RFLP Method in West Azerbaijan - Iran Iranian Journal of Parasitology Hydatid cyst ITS1 RFLP-PCR Strain |
author_facet |
Haleh Hanifian Kambiz Diba Khosrow Hazrati Tappeh Habib Mohammadzadeh Rahim Mahmoudlou |
author_sort |
Haleh Hanifian |
title |
Identification of Echinococcus granulosus Strains in Isolated Hydatid Cyst Specimens from Animals by PCR-RFLP Method in West Azerbaijan - Iran |
title_short |
Identification of Echinococcus granulosus Strains in Isolated Hydatid Cyst Specimens from Animals by PCR-RFLP Method in West Azerbaijan - Iran |
title_full |
Identification of Echinococcus granulosus Strains in Isolated Hydatid Cyst Specimens from Animals by PCR-RFLP Method in West Azerbaijan - Iran |
title_fullStr |
Identification of Echinococcus granulosus Strains in Isolated Hydatid Cyst Specimens from Animals by PCR-RFLP Method in West Azerbaijan - Iran |
title_full_unstemmed |
Identification of Echinococcus granulosus Strains in Isolated Hydatid Cyst Specimens from Animals by PCR-RFLP Method in West Azerbaijan - Iran |
title_sort |
identification of echinococcus granulosus strains in isolated hydatid cyst specimens from animals by pcr-rflp method in west azerbaijan - iran |
publisher |
Tehran University of Medical Sciences |
series |
Iranian Journal of Parasitology |
issn |
1735-7020 2008-238X |
publishDate |
2013-09-01 |
description |
Background: The aim of this study was DNA extraction from protosco-lecses of Echinococcus granulosus and identification of these strains in West- Azerbaijan Province, north western Iran.
Methods: Thirty one livestock isolates from sheep and cattle were collected from abattoirs of the province. To investigate the genetic variation of the isolates, after DNA extraction by Glass beads-phenol chloroform method; PCR-RLFP analysis of rDNA-ITS1 was performed using three different re-striction enzymes of Taq 1, Rsa 1 and Alu 1.
Result: Amplified PCR products for all isolates were 1000bp band which is expected band in sheep strains (G1-G3 complex). The results of RFLP analy-sis also were the same for all isolates. PCR-RFLP patterns restriction en-zymes were identical as follows, Rsa1 bands under UV showed two bands approximately 655bp and 345bp. Alu1 bands were as follows: two approx-imately 800bp and 200bp and Taq1 did not cut any region and bands were approximately 1000 bp in all samples.
Conclusions: Based on PCR-RFLP patterns of ITS1 fragment produced with endonucleases enzyme digestion in animal isolates, it can be concluded that a single strain of E. granulosus (sheep strain or G1-G3 complex) is domi-nant genotype in this province.
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topic |
Hydatid cyst ITS1 RFLP-PCR Strain |
url |
https://ijpa.tums.ac.ir/index.php/ijpa/article/view/481 |
work_keys_str_mv |
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