Spinning-Spot Shadowless TIRF Microscopy.

Total internal reflection fluorescence (TIRF) microscopy is a powerful tool for visualizing near-membrane cellular structures and processes, including imaging of local Ca2+ transients with single-channel resolution. TIRF is most commonly implemented in epi-fluorescence mode, whereby laser excitation...

Full description

Bibliographic Details
Main Authors: Kyle L Ellefsen, Joseph L Dynes, Ian Parker
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4550233?pdf=render