An Electrochemical Enzyme Biosensor for Ammonium Detection in Aquaculture Using Screen-Printed Electrode Modified by Gold Nanoparticle/Polymethylene Blue
A SPEC/AuNPs/PMB modified electrode was prepared by electrodeposition and electro-polymerization. The electrochemical behavior of reduced nicotinamide adenine dinucleotide (NADH) on the surface of the modified electrode was studied by cyclic voltammetry. A certain amount of substrate and glutamate d...
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doaj-7ee87d14889f4b9b937368ef95950c752021-09-25T23:48:08ZengMDPI AGBiosensors2079-63742021-09-011133533510.3390/bios11090335An Electrochemical Enzyme Biosensor for Ammonium Detection in Aquaculture Using Screen-Printed Electrode Modified by Gold Nanoparticle/Polymethylene BlueCong Wang0Tan Wang1Zhen Li2Xianbao Xu3Xiaoshuan Zhang4Daoliang Li5National Innovation Center for Digital Fishery, China Agricultural University, Beijing 100083, ChinaNational Innovation Center for Digital Fishery, China Agricultural University, Beijing 100083, ChinaNational Innovation Center for Digital Fishery, China Agricultural University, Beijing 100083, ChinaNational Innovation Center for Digital Fishery, China Agricultural University, Beijing 100083, ChinaNational Innovation Center for Digital Fishery, China Agricultural University, Beijing 100083, ChinaNational Innovation Center for Digital Fishery, China Agricultural University, Beijing 100083, ChinaA SPEC/AuNPs/PMB modified electrode was prepared by electrodeposition and electro-polymerization. The electrochemical behavior of reduced nicotinamide adenine dinucleotide (NADH) on the surface of the modified electrode was studied by cyclic voltammetry. A certain amount of substrate and glutamate dehydrogenase (GLDH) were coated on the modified electrode to form a functional enzyme membrane. The ammonia nitrogen in the water sample could be calculated indirectly by measuring the consumption of NADH in the reaction. The results showed that the strength of electro-catalytic current signal was increased by two times; the catalytic oxidation potential was shifted to the left by 0.5 V, and the anti-interference ability of the sensor was enhanced. The optimum substrate concentration and enzyme loading were determined as 1.3 mM NADH, 28 mM α-Ketoglutarate and 2.0 U GLDH, respectively. The homemade ceramic heating plate controlled the working electrode to work at 37 °C. A pH compensation algorithm based on piecewise linear interpolation could reduce the measurement error to less than 3.29 μM. The biosensor exhibited good linearity in the range of 0~300 μM with a detection limit of 0.65 μM NH<sub>4</sub><sup>+</sup>. Compared with standard Nessler’s method, the recoveries were 93.71~105.92%. The biosensor was found to be stable for at least 14 days when refrigerated and sealed at 4 °C.https://www.mdpi.com/2079-6374/11/9/335biosensorglutamate dehydrogenasemethylene bluescreen-printed electrodeammonium |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cong Wang Tan Wang Zhen Li Xianbao Xu Xiaoshuan Zhang Daoliang Li |
spellingShingle |
Cong Wang Tan Wang Zhen Li Xianbao Xu Xiaoshuan Zhang Daoliang Li An Electrochemical Enzyme Biosensor for Ammonium Detection in Aquaculture Using Screen-Printed Electrode Modified by Gold Nanoparticle/Polymethylene Blue Biosensors biosensor glutamate dehydrogenase methylene blue screen-printed electrode ammonium |
author_facet |
Cong Wang Tan Wang Zhen Li Xianbao Xu Xiaoshuan Zhang Daoliang Li |
author_sort |
Cong Wang |
title |
An Electrochemical Enzyme Biosensor for Ammonium Detection in Aquaculture Using Screen-Printed Electrode Modified by Gold Nanoparticle/Polymethylene Blue |
title_short |
An Electrochemical Enzyme Biosensor for Ammonium Detection in Aquaculture Using Screen-Printed Electrode Modified by Gold Nanoparticle/Polymethylene Blue |
title_full |
An Electrochemical Enzyme Biosensor for Ammonium Detection in Aquaculture Using Screen-Printed Electrode Modified by Gold Nanoparticle/Polymethylene Blue |
title_fullStr |
An Electrochemical Enzyme Biosensor for Ammonium Detection in Aquaculture Using Screen-Printed Electrode Modified by Gold Nanoparticle/Polymethylene Blue |
title_full_unstemmed |
An Electrochemical Enzyme Biosensor for Ammonium Detection in Aquaculture Using Screen-Printed Electrode Modified by Gold Nanoparticle/Polymethylene Blue |
title_sort |
electrochemical enzyme biosensor for ammonium detection in aquaculture using screen-printed electrode modified by gold nanoparticle/polymethylene blue |
publisher |
MDPI AG |
series |
Biosensors |
issn |
2079-6374 |
publishDate |
2021-09-01 |
description |
A SPEC/AuNPs/PMB modified electrode was prepared by electrodeposition and electro-polymerization. The electrochemical behavior of reduced nicotinamide adenine dinucleotide (NADH) on the surface of the modified electrode was studied by cyclic voltammetry. A certain amount of substrate and glutamate dehydrogenase (GLDH) were coated on the modified electrode to form a functional enzyme membrane. The ammonia nitrogen in the water sample could be calculated indirectly by measuring the consumption of NADH in the reaction. The results showed that the strength of electro-catalytic current signal was increased by two times; the catalytic oxidation potential was shifted to the left by 0.5 V, and the anti-interference ability of the sensor was enhanced. The optimum substrate concentration and enzyme loading were determined as 1.3 mM NADH, 28 mM α-Ketoglutarate and 2.0 U GLDH, respectively. The homemade ceramic heating plate controlled the working electrode to work at 37 °C. A pH compensation algorithm based on piecewise linear interpolation could reduce the measurement error to less than 3.29 μM. The biosensor exhibited good linearity in the range of 0~300 μM with a detection limit of 0.65 μM NH<sub>4</sub><sup>+</sup>. Compared with standard Nessler’s method, the recoveries were 93.71~105.92%. The biosensor was found to be stable for at least 14 days when refrigerated and sealed at 4 °C. |
topic |
biosensor glutamate dehydrogenase methylene blue screen-printed electrode ammonium |
url |
https://www.mdpi.com/2079-6374/11/9/335 |
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