Assessment of the Polyphenol Indices and Antioxidant Capacity for Beers and Wines Using a Tyrosinase-Based Biosensor Prepared by Sinusoidal Current Method
The application of a novel Poly(3,4-ethylenedioxythiophene)-Tyrosinase/Sonogel-Carbon electrode (PEDOT-Tyr/SNGC) biosensor to beers and wines analysis is proposed. This biosensor implies a new Sinusoidal Current (SC) electrodeposition method to immobilize the enzyme generating a nanostructure surfac...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2018-12-01
|
Series: | Sensors |
Subjects: | |
Online Access: | http://www.mdpi.com/1424-8220/19/1/66 |
id |
doaj-7efa71b8a3714ff0b2dbb62646c914de |
---|---|
record_format |
Article |
spelling |
doaj-7efa71b8a3714ff0b2dbb62646c914de2020-11-25T00:44:10ZengMDPI AGSensors1424-82202018-12-011916610.3390/s19010066s19010066Assessment of the Polyphenol Indices and Antioxidant Capacity for Beers and Wines Using a Tyrosinase-Based Biosensor Prepared by Sinusoidal Current MethodJuan José García-Guzmán0David López-Iglesias1Laura Cubillana-Aguilera2Cecilia Lete3Stelian Lupu4José María Palacios-Santander5Dolores Bellido-Milla6Institute of Research on Electron Microscopy and Materials (IMEYMAT), Department of Analytical Chemistry, Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEIMAR), University of Cadiz, Polígono del Río San Pedro, S/N. 11510 Puerto Real, 11001 Cadiz, SpainInstitute of Research on Electron Microscopy and Materials (IMEYMAT), Department of Analytical Chemistry, Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEIMAR), University of Cadiz, Polígono del Río San Pedro, S/N. 11510 Puerto Real, 11001 Cadiz, SpainInstitute of Research on Electron Microscopy and Materials (IMEYMAT), Department of Analytical Chemistry, Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEIMAR), University of Cadiz, Polígono del Río San Pedro, S/N. 11510 Puerto Real, 11001 Cadiz, SpainInstitute of Physical Chemistry “Ilie Murgulescu” of the Romanian Academy, 202 Splaiul Independentei, 060021 Bucharest, RomaniaDepartment of Analytical Chemistry and Environmental Engineering, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, RomaniaInstitute of Research on Electron Microscopy and Materials (IMEYMAT), Department of Analytical Chemistry, Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEIMAR), University of Cadiz, Polígono del Río San Pedro, S/N. 11510 Puerto Real, 11001 Cadiz, SpainInstitute of Research on Electron Microscopy and Materials (IMEYMAT), Department of Analytical Chemistry, Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEIMAR), University of Cadiz, Polígono del Río San Pedro, S/N. 11510 Puerto Real, 11001 Cadiz, SpainThe application of a novel Poly(3,4-ethylenedioxythiophene)-Tyrosinase/Sonogel-Carbon electrode (PEDOT-Tyr/SNGC) biosensor to beers and wines analysis is proposed. This biosensor implies a new Sinusoidal Current (SC) electrodeposition method to immobilize the enzyme generating a nanostructure surface. The biosensors were characterized electrochemically, employing cyclic voltammetry and electrochemical impedance spectroscopy. Sensitivity, limit of detection, and correlation coefficients of the linear fitting were 2.40 × 10−4 µA·µM−1, 4.33 µM, and R2 = 0.9987, respectively. Caffeic acid is used as the reference polyphenol. A sampling of nine beers (four lager, three stout, and two non-alcoholic beers), and four wines (three red and one white wine) purchased in a local store was performed. The Polyphenol indeces for beers and wines have been assessed using the proposed biosensor, and the obtained values are in agreement with the literature data. Antioxidant properties of the samples using the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical spectrophotometric method were also evaluated. The correlation between the polyphenol index and the antioxidant capacity was obtained for beers and wines.http://www.mdpi.com/1424-8220/19/1/66sonogel–carbon based biosensorsinusoidal current methodpolyphenol indexantioxidant capacitybeer and wine |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Juan José García-Guzmán David López-Iglesias Laura Cubillana-Aguilera Cecilia Lete Stelian Lupu José María Palacios-Santander Dolores Bellido-Milla |
spellingShingle |
Juan José García-Guzmán David López-Iglesias Laura Cubillana-Aguilera Cecilia Lete Stelian Lupu José María Palacios-Santander Dolores Bellido-Milla Assessment of the Polyphenol Indices and Antioxidant Capacity for Beers and Wines Using a Tyrosinase-Based Biosensor Prepared by Sinusoidal Current Method Sensors sonogel–carbon based biosensor sinusoidal current method polyphenol index antioxidant capacity beer and wine |
author_facet |
Juan José García-Guzmán David López-Iglesias Laura Cubillana-Aguilera Cecilia Lete Stelian Lupu José María Palacios-Santander Dolores Bellido-Milla |
author_sort |
Juan José García-Guzmán |
title |
Assessment of the Polyphenol Indices and Antioxidant Capacity for Beers and Wines Using a Tyrosinase-Based Biosensor Prepared by Sinusoidal Current Method |
title_short |
Assessment of the Polyphenol Indices and Antioxidant Capacity for Beers and Wines Using a Tyrosinase-Based Biosensor Prepared by Sinusoidal Current Method |
title_full |
Assessment of the Polyphenol Indices and Antioxidant Capacity for Beers and Wines Using a Tyrosinase-Based Biosensor Prepared by Sinusoidal Current Method |
title_fullStr |
Assessment of the Polyphenol Indices and Antioxidant Capacity for Beers and Wines Using a Tyrosinase-Based Biosensor Prepared by Sinusoidal Current Method |
title_full_unstemmed |
Assessment of the Polyphenol Indices and Antioxidant Capacity for Beers and Wines Using a Tyrosinase-Based Biosensor Prepared by Sinusoidal Current Method |
title_sort |
assessment of the polyphenol indices and antioxidant capacity for beers and wines using a tyrosinase-based biosensor prepared by sinusoidal current method |
publisher |
MDPI AG |
series |
Sensors |
issn |
1424-8220 |
publishDate |
2018-12-01 |
description |
The application of a novel Poly(3,4-ethylenedioxythiophene)-Tyrosinase/Sonogel-Carbon electrode (PEDOT-Tyr/SNGC) biosensor to beers and wines analysis is proposed. This biosensor implies a new Sinusoidal Current (SC) electrodeposition method to immobilize the enzyme generating a nanostructure surface. The biosensors were characterized electrochemically, employing cyclic voltammetry and electrochemical impedance spectroscopy. Sensitivity, limit of detection, and correlation coefficients of the linear fitting were 2.40 × 10−4 µA·µM−1, 4.33 µM, and R2 = 0.9987, respectively. Caffeic acid is used as the reference polyphenol. A sampling of nine beers (four lager, three stout, and two non-alcoholic beers), and four wines (three red and one white wine) purchased in a local store was performed. The Polyphenol indeces for beers and wines have been assessed using the proposed biosensor, and the obtained values are in agreement with the literature data. Antioxidant properties of the samples using the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical spectrophotometric method were also evaluated. The correlation between the polyphenol index and the antioxidant capacity was obtained for beers and wines. |
topic |
sonogel–carbon based biosensor sinusoidal current method polyphenol index antioxidant capacity beer and wine |
url |
http://www.mdpi.com/1424-8220/19/1/66 |
work_keys_str_mv |
AT juanjosegarciaguzman assessmentofthepolyphenolindicesandantioxidantcapacityforbeersandwinesusingatyrosinasebasedbiosensorpreparedbysinusoidalcurrentmethod AT davidlopeziglesias assessmentofthepolyphenolindicesandantioxidantcapacityforbeersandwinesusingatyrosinasebasedbiosensorpreparedbysinusoidalcurrentmethod AT lauracubillanaaguilera assessmentofthepolyphenolindicesandantioxidantcapacityforbeersandwinesusingatyrosinasebasedbiosensorpreparedbysinusoidalcurrentmethod AT cecilialete assessmentofthepolyphenolindicesandantioxidantcapacityforbeersandwinesusingatyrosinasebasedbiosensorpreparedbysinusoidalcurrentmethod AT stelianlupu assessmentofthepolyphenolindicesandantioxidantcapacityforbeersandwinesusingatyrosinasebasedbiosensorpreparedbysinusoidalcurrentmethod AT josemariapalaciossantander assessmentofthepolyphenolindicesandantioxidantcapacityforbeersandwinesusingatyrosinasebasedbiosensorpreparedbysinusoidalcurrentmethod AT doloresbellidomilla assessmentofthepolyphenolindicesandantioxidantcapacityforbeersandwinesusingatyrosinasebasedbiosensorpreparedbysinusoidalcurrentmethod |
_version_ |
1725276067244015616 |