Invasion of <it>Ureaplasma diversum </it>in bovine spermatozoids
<p>Abstract</p> <p>Background</p> <p><it>Ureaplasma diversum </it>has been associated with infertility in cows. In bulls, this mollicute colonizes the prepuce and distal portion of the urethra and may infect sperm cells. The aim of this study is to analyze &...
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doaj-7efcfdbf23ff47d98453db3d1fb7258a2020-11-25T01:54:26ZengBMCBMC Research Notes1756-05002011-10-014145510.1186/1756-0500-4-455Invasion of <it>Ureaplasma diversum </it>in bovine spermatozoidsTimenetsky JorgeAssumpção Mayra EOMachado-Santelli Gláucia MMarques LucasCortez Beatriz AOliveira Rosângela CYamaguti MaurícioBuzinhani Melissa<p>Abstract</p> <p>Background</p> <p><it>Ureaplasma diversum </it>has been associated with infertility in cows. In bulls, this mollicute colonizes the prepuce and distal portion of the urethra and may infect sperm cells. The aim of this study is to analyze <it>in vitro </it>interaction of <it>U. diversum </it>isolates and ATCC strains with bovine spermatozoids. The interactions were observed by confocal microscopy and the gentamycin internalization assay.</p> <p>Findings</p> <p><it>U. diversum </it>were able to adhere to and invade spermatozoids after 30 min of infection. The gentamicin resistance assay confirmed the intracellularity and survival of <it>U. diversum </it>in bovine spermatozoids.</p> <p>Conclusions</p> <p>The intracellular nature of bovine ureaplasma identifies a new difficulty to control the reproductive of these animals.</p> http://www.biomedcentral.com/1756-0500/4/455<it>Ureaplasma diversum</it>bovine spermatozoidinvasionconfocal microscopy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Timenetsky Jorge Assumpção Mayra EO Machado-Santelli Gláucia M Marques Lucas Cortez Beatriz A Oliveira Rosângela C Yamaguti Maurício Buzinhani Melissa |
spellingShingle |
Timenetsky Jorge Assumpção Mayra EO Machado-Santelli Gláucia M Marques Lucas Cortez Beatriz A Oliveira Rosângela C Yamaguti Maurício Buzinhani Melissa Invasion of <it>Ureaplasma diversum </it>in bovine spermatozoids BMC Research Notes <it>Ureaplasma diversum</it> bovine spermatozoid invasion confocal microscopy |
author_facet |
Timenetsky Jorge Assumpção Mayra EO Machado-Santelli Gláucia M Marques Lucas Cortez Beatriz A Oliveira Rosângela C Yamaguti Maurício Buzinhani Melissa |
author_sort |
Timenetsky Jorge |
title |
Invasion of <it>Ureaplasma diversum </it>in bovine spermatozoids |
title_short |
Invasion of <it>Ureaplasma diversum </it>in bovine spermatozoids |
title_full |
Invasion of <it>Ureaplasma diversum </it>in bovine spermatozoids |
title_fullStr |
Invasion of <it>Ureaplasma diversum </it>in bovine spermatozoids |
title_full_unstemmed |
Invasion of <it>Ureaplasma diversum </it>in bovine spermatozoids |
title_sort |
invasion of <it>ureaplasma diversum </it>in bovine spermatozoids |
publisher |
BMC |
series |
BMC Research Notes |
issn |
1756-0500 |
publishDate |
2011-10-01 |
description |
<p>Abstract</p> <p>Background</p> <p><it>Ureaplasma diversum </it>has been associated with infertility in cows. In bulls, this mollicute colonizes the prepuce and distal portion of the urethra and may infect sperm cells. The aim of this study is to analyze <it>in vitro </it>interaction of <it>U. diversum </it>isolates and ATCC strains with bovine spermatozoids. The interactions were observed by confocal microscopy and the gentamycin internalization assay.</p> <p>Findings</p> <p><it>U. diversum </it>were able to adhere to and invade spermatozoids after 30 min of infection. The gentamicin resistance assay confirmed the intracellularity and survival of <it>U. diversum </it>in bovine spermatozoids.</p> <p>Conclusions</p> <p>The intracellular nature of bovine ureaplasma identifies a new difficulty to control the reproductive of these animals.</p> |
topic |
<it>Ureaplasma diversum</it> bovine spermatozoid invasion confocal microscopy |
url |
http://www.biomedcentral.com/1756-0500/4/455 |
work_keys_str_mv |
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