Effects of exogenous zinc on the photosynthesis and carbonic anhydrase activity of millet (Setaria italica L.)
This study aimed to evaluate the effects of Zn on the growth safety and activity of carbonic anhydrase (CA) in foxtail millet (Setaria italica L.). The photosynthetic characteristics, CA activity, and relative gene expression of different varieties of millet at the seedling stage were studied by spr...
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Academy of Sciences of the Czech Republic, Institute of Experimental Botany
2020-06-01
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doaj-7f096a8e862f49ae9dcda4110ce836382020-11-25T03:10:38ZengAcademy of Sciences of the Czech Republic, Institute of Experimental BotanyPhotosynthetica0300-36041573-90582020-06-0158371271910.32615/ps.2020.018phs-202003-0004Effects of exogenous zinc on the photosynthesis and carbonic anhydrase activity of millet (Setaria italica L.)M.L. CAO0Y.X. LI1H.L. DU2College of Agricultural Science, Shanxi Agricultural University, Taigu, 030801 Shanxi, ChinaCollege of Agricultural Science, Shanxi Agricultural University, Taigu, 030801 Shanxi, ChinaCollege of Arts and Sciences, Shanxi Agricultural University, Taigu, 030801 Shanxi, ChinaThis study aimed to evaluate the effects of Zn on the growth safety and activity of carbonic anhydrase (CA) in foxtail millet (Setaria italica L.). The photosynthetic characteristics, CA activity, and relative gene expression of different varieties of millet at the seedling stage were studied by spraying Zn solution under pot experiment and indoor culture conditions. Results showed that spraying low-concentration Zn solution (20, 40, and 60 mg L-1) reduced malondialdehyde content and intercellular CO2 concentration (Ci) but increased antioxidant enzyme activity, pigment content, and photosynthetic gas-exchange parameters (net photosynthetic rate, stomatal conductance, transpiration rate, except for Ci); meanwhile, spraying high Zn concentration (80 and 100 mg L-1) exerted opposite effects. The optimal growth of millet was achieved when the Zn concentration was 40 mg L-1. At this concentration, CA activity increased and β-CA family expression was upregulated, which exerted little or no effect on other CA families. Compared to Zhangzagu 10 (zinc-resistant variety), Jingu 21 (zinc-sensitive variety) showed a more significant change. This study may serve as a reference for further research on the function of CA and physiological processes, such as photosynthesis in millet, and a theoretical basis for the effective use of Zn fertilizer in millet.https://ps.ueb.cas.cz/artkey/phs-202003-0004_effects-of-exogenous-zinc-on-the-photosynthesis-and-carbonic-anhydrase-activity-of-millet-setaria-italica-l.phpcarbonic anhydrase gene familyperoxidasephotosynthetic pigmentsuperoxide dismutase |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
M.L. CAO Y.X. LI H.L. DU |
spellingShingle |
M.L. CAO Y.X. LI H.L. DU Effects of exogenous zinc on the photosynthesis and carbonic anhydrase activity of millet (Setaria italica L.) Photosynthetica carbonic anhydrase gene family peroxidase photosynthetic pigment superoxide dismutase |
author_facet |
M.L. CAO Y.X. LI H.L. DU |
author_sort |
M.L. CAO |
title |
Effects of exogenous zinc on the photosynthesis and carbonic anhydrase activity of millet (Setaria italica L.) |
title_short |
Effects of exogenous zinc on the photosynthesis and carbonic anhydrase activity of millet (Setaria italica L.) |
title_full |
Effects of exogenous zinc on the photosynthesis and carbonic anhydrase activity of millet (Setaria italica L.) |
title_fullStr |
Effects of exogenous zinc on the photosynthesis and carbonic anhydrase activity of millet (Setaria italica L.) |
title_full_unstemmed |
Effects of exogenous zinc on the photosynthesis and carbonic anhydrase activity of millet (Setaria italica L.) |
title_sort |
effects of exogenous zinc on the photosynthesis and carbonic anhydrase activity of millet (setaria italica l.) |
publisher |
Academy of Sciences of the Czech Republic, Institute of Experimental Botany |
series |
Photosynthetica |
issn |
0300-3604 1573-9058 |
publishDate |
2020-06-01 |
description |
This study aimed to evaluate the effects of Zn on the growth safety and activity of carbonic anhydrase (CA) in foxtail millet (Setaria italica L.). The photosynthetic characteristics, CA activity, and relative gene expression of different varieties of millet at the seedling stage were studied by spraying Zn solution under pot experiment and indoor culture conditions. Results showed that spraying low-concentration Zn solution (20, 40, and 60 mg L-1) reduced malondialdehyde content and intercellular CO2 concentration (Ci) but increased antioxidant enzyme activity, pigment content, and photosynthetic gas-exchange parameters (net photosynthetic rate, stomatal conductance, transpiration rate, except for Ci); meanwhile, spraying high Zn concentration (80 and 100 mg L-1) exerted opposite effects. The optimal growth of millet was achieved when the Zn concentration was 40 mg L-1. At this concentration, CA activity increased and β-CA family expression was upregulated, which exerted little or no effect on other CA families. Compared to Zhangzagu 10 (zinc-resistant variety), Jingu 21 (zinc-sensitive variety) showed a more significant change. This study may serve as a reference for further research on the function of CA and physiological processes, such as photosynthesis in millet, and a theoretical basis for the effective use of Zn fertilizer in millet. |
topic |
carbonic anhydrase gene family peroxidase photosynthetic pigment superoxide dismutase |
url |
https://ps.ueb.cas.cz/artkey/phs-202003-0004_effects-of-exogenous-zinc-on-the-photosynthesis-and-carbonic-anhydrase-activity-of-millet-setaria-italica-l.php |
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