Genomic and secretomic analyses reveal unique features of the lignocellulolytic enzyme system of Penicillium decumbens.
Many Penicillium species could produce extracellular enzyme systems with good lignocellulose hydrolysis performance. However, these species and their enzyme systems are still poorly understood and explored due to the lacking of genetic information. Here, we present the genomic and secretomic analyse...
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2013-01-01
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doaj-7f098f6063a840c0a7f2018783814f512021-03-03T23:46:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0182e5518510.1371/journal.pone.0055185Genomic and secretomic analyses reveal unique features of the lignocellulolytic enzyme system of Penicillium decumbens.Guodong LiuLei ZhangXiaomin WeiGen ZouYuqi QinLiang MaJie LiHuajun ZhengShengyue WangChengshu WangLuying XunGuo-Ping ZhaoZhihua ZhouYinbo QuMany Penicillium species could produce extracellular enzyme systems with good lignocellulose hydrolysis performance. However, these species and their enzyme systems are still poorly understood and explored due to the lacking of genetic information. Here, we present the genomic and secretomic analyses of Penicillium decumbens that has been used in industrial production of lignocellulolytic enzymes in China for more than fifteen years. Comparative genomics analysis with the phylogenetically most similar species Penicillium chrysogenum revealed that P. decumbens has evolved with more genes involved in plant cell wall degradation, but fewer genes in cellular metabolism and regulation. Compared with the widely used cellulase producer Trichoderma reesei, P. decumbens has a lignocellulolytic enzyme system with more diverse components, particularly for cellulose binding domain-containing proteins and hemicellulases. Further, proteomic analysis of secretomes revealed that P. decumbens produced significantly more lignocellulolytic enzymes in the medium with cellulose-wheat bran as the carbon source than with glucose. The results expand our knowledge on the genetic information of lignocellulolytic enzyme systems in Penicillium species, and will facilitate rational strain improvement for the production of highly efficient enzyme systems used in lignocellulose utilization from Penicillium species.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23383313/pdf/?tool=EBI |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Guodong Liu Lei Zhang Xiaomin Wei Gen Zou Yuqi Qin Liang Ma Jie Li Huajun Zheng Shengyue Wang Chengshu Wang Luying Xun Guo-Ping Zhao Zhihua Zhou Yinbo Qu |
spellingShingle |
Guodong Liu Lei Zhang Xiaomin Wei Gen Zou Yuqi Qin Liang Ma Jie Li Huajun Zheng Shengyue Wang Chengshu Wang Luying Xun Guo-Ping Zhao Zhihua Zhou Yinbo Qu Genomic and secretomic analyses reveal unique features of the lignocellulolytic enzyme system of Penicillium decumbens. PLoS ONE |
author_facet |
Guodong Liu Lei Zhang Xiaomin Wei Gen Zou Yuqi Qin Liang Ma Jie Li Huajun Zheng Shengyue Wang Chengshu Wang Luying Xun Guo-Ping Zhao Zhihua Zhou Yinbo Qu |
author_sort |
Guodong Liu |
title |
Genomic and secretomic analyses reveal unique features of the lignocellulolytic enzyme system of Penicillium decumbens. |
title_short |
Genomic and secretomic analyses reveal unique features of the lignocellulolytic enzyme system of Penicillium decumbens. |
title_full |
Genomic and secretomic analyses reveal unique features of the lignocellulolytic enzyme system of Penicillium decumbens. |
title_fullStr |
Genomic and secretomic analyses reveal unique features of the lignocellulolytic enzyme system of Penicillium decumbens. |
title_full_unstemmed |
Genomic and secretomic analyses reveal unique features of the lignocellulolytic enzyme system of Penicillium decumbens. |
title_sort |
genomic and secretomic analyses reveal unique features of the lignocellulolytic enzyme system of penicillium decumbens. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
Many Penicillium species could produce extracellular enzyme systems with good lignocellulose hydrolysis performance. However, these species and their enzyme systems are still poorly understood and explored due to the lacking of genetic information. Here, we present the genomic and secretomic analyses of Penicillium decumbens that has been used in industrial production of lignocellulolytic enzymes in China for more than fifteen years. Comparative genomics analysis with the phylogenetically most similar species Penicillium chrysogenum revealed that P. decumbens has evolved with more genes involved in plant cell wall degradation, but fewer genes in cellular metabolism and regulation. Compared with the widely used cellulase producer Trichoderma reesei, P. decumbens has a lignocellulolytic enzyme system with more diverse components, particularly for cellulose binding domain-containing proteins and hemicellulases. Further, proteomic analysis of secretomes revealed that P. decumbens produced significantly more lignocellulolytic enzymes in the medium with cellulose-wheat bran as the carbon source than with glucose. The results expand our knowledge on the genetic information of lignocellulolytic enzyme systems in Penicillium species, and will facilitate rational strain improvement for the production of highly efficient enzyme systems used in lignocellulose utilization from Penicillium species. |
url |
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23383313/pdf/?tool=EBI |
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