Comparison of Two Solid-Phase Extraction (SPE) Methods for the Identification and Quantification of Porcine Retinal Protein Markers by LC-MS/MS

Comparison of Two Solid-Phase Extraction (SPE) Methods for the Identification and Quantification of Porcine Retinal Protein Markers by LC-MS/MS

Proper sample preparation protocols represent a critical step for liquid chromatography-mass spectrometry (LC-MS)-based proteomic study designs and influence the speed, performance and automation of high-throughput data acquisition. The main objective of this study was to compare two commercial soli...

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Bibliographic Details
Main Authors: Carsten Schmelter, Sebastian Funke, Jana Treml, Anja Beschnitt, Natarajan Perumal, Caroline Manicam, Norbert Pfeiffer, Franz H. Grus
Format: Article
Language:English
Published: MDPI AG 2018-12-01
Series:International Journal of Molecular Sciences
Subjects:
mass spectrometry
glaucoma animal model
biomarkers
sample clean-up
ZIPTIP<sup>®</sup> C18 pipette tips
SOLAμTM HRP SPE spin plates
Online Access:https://www.mdpi.com/1422-0067/19/12/3847

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