Prevalence of Enteropathogens and Virulence Traits in Brazilian Children With and Without Diarrhea
The use of molecular diagnostics for pathogen detection in epidemiological studies have allowed us to get a wider view of the pathogens associated with diarrhea, but the presence of enteropathogens in asymptomatic individuals has raised several challenges in understanding the etiology of diarrhea, a...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2020-09-01
|
Series: | Frontiers in Cellular and Infection Microbiology |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fcimb.2020.549919/full |
id |
doaj-7f0eefa6ec684ac394a69addacc89222 |
---|---|
record_format |
Article |
spelling |
doaj-7f0eefa6ec684ac394a69addacc892222020-11-25T03:59:04ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882020-09-011010.3389/fcimb.2020.549919549919Prevalence of Enteropathogens and Virulence Traits in Brazilian Children With and Without DiarrheaVictor R. Merino0Viviane Nakano1Sabine Delannoy2Patrick Fach3Gabriela G. F. Alberca4Mauricio J. Farfan5Roxane M. F. Piazza6Mario J. Avila-Campos7Laboratório de Anaeróbios, Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade of São Paulo, São Paulo, BrazilLaboratório de Anaeróbios, Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade of São Paulo, São Paulo, BrazilAgence Nationale de Sécurité Sanitaire de l'Alimentation, de l'Environnement et du Travail, Food Safety Laboratory, Université Paris-Est, Champs-sur-Marne, FranceAgence Nationale de Sécurité Sanitaire de l'Alimentation, de l'Environnement et du Travail, Food Safety Laboratory, Université Paris-Est, Champs-sur-Marne, FranceLaboratório de Anaeróbios, Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade of São Paulo, São Paulo, BrazilDepartamento de Pediatría y Cirugía Infantil, Facultad de Medicina, Campus Oriente-Hospital Dr. Luis Calvo Mackenna, Universidad de Chile, Santiago, ChileLaboratório de Bacteriologia, Instituto Butantan, São Paulo, BrazilLaboratório de Anaeróbios, Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade of São Paulo, São Paulo, BrazilThe use of molecular diagnostics for pathogen detection in epidemiological studies have allowed us to get a wider view of the pathogens associated with diarrhea, but the presence of enteropathogens in asymptomatic individuals has raised several challenges in understanding the etiology of diarrhea, and the use of these platforms in clinical diagnosis as well. To characterize the presence of the most relevant bacterial enteropathogens in diarrheal episodes, we evaluated here the prevalence of diarrheagenic E. coli pathotypes, Salmonella spp., and Yersinia enterocolitica in stool samples of children with and without diarrhea using real-time quantitative PCR (qPCR). We found that the presence of genetic markers associated with bacterial pathogens was significantly higher in stool samples from the diarrhea group compared to the control (P < 0.001). Bacterial loads in samples positive for eae and aggR markers were also determined. Compared to samples from asymptomatic children, a significantly higher number of copies of the eae gene were found in diarrhea samples. Also, the presence of genetic markers associated with STEC strains with clinical significance was evaluated in eae-positive samples by high-throughput real-time PCR. The data presented herein demonstrated that asymptomatic children of an urban area in Brazil might be enteropathogen reservoirs, especially for STEC.https://www.frontiersin.org/article/10.3389/fcimb.2020.549919/fullenteric pathogensEscherichia coli pathotypesdiarrheal childrenhealthy childrenmolecular diagnostics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Victor R. Merino Viviane Nakano Sabine Delannoy Patrick Fach Gabriela G. F. Alberca Mauricio J. Farfan Roxane M. F. Piazza Mario J. Avila-Campos |
spellingShingle |
Victor R. Merino Viviane Nakano Sabine Delannoy Patrick Fach Gabriela G. F. Alberca Mauricio J. Farfan Roxane M. F. Piazza Mario J. Avila-Campos Prevalence of Enteropathogens and Virulence Traits in Brazilian Children With and Without Diarrhea Frontiers in Cellular and Infection Microbiology enteric pathogens Escherichia coli pathotypes diarrheal children healthy children molecular diagnostics |
author_facet |
Victor R. Merino Viviane Nakano Sabine Delannoy Patrick Fach Gabriela G. F. Alberca Mauricio J. Farfan Roxane M. F. Piazza Mario J. Avila-Campos |
author_sort |
Victor R. Merino |
title |
Prevalence of Enteropathogens and Virulence Traits in Brazilian Children With and Without Diarrhea |
title_short |
Prevalence of Enteropathogens and Virulence Traits in Brazilian Children With and Without Diarrhea |
title_full |
Prevalence of Enteropathogens and Virulence Traits in Brazilian Children With and Without Diarrhea |
title_fullStr |
Prevalence of Enteropathogens and Virulence Traits in Brazilian Children With and Without Diarrhea |
title_full_unstemmed |
Prevalence of Enteropathogens and Virulence Traits in Brazilian Children With and Without Diarrhea |
title_sort |
prevalence of enteropathogens and virulence traits in brazilian children with and without diarrhea |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular and Infection Microbiology |
issn |
2235-2988 |
publishDate |
2020-09-01 |
description |
The use of molecular diagnostics for pathogen detection in epidemiological studies have allowed us to get a wider view of the pathogens associated with diarrhea, but the presence of enteropathogens in asymptomatic individuals has raised several challenges in understanding the etiology of diarrhea, and the use of these platforms in clinical diagnosis as well. To characterize the presence of the most relevant bacterial enteropathogens in diarrheal episodes, we evaluated here the prevalence of diarrheagenic E. coli pathotypes, Salmonella spp., and Yersinia enterocolitica in stool samples of children with and without diarrhea using real-time quantitative PCR (qPCR). We found that the presence of genetic markers associated with bacterial pathogens was significantly higher in stool samples from the diarrhea group compared to the control (P < 0.001). Bacterial loads in samples positive for eae and aggR markers were also determined. Compared to samples from asymptomatic children, a significantly higher number of copies of the eae gene were found in diarrhea samples. Also, the presence of genetic markers associated with STEC strains with clinical significance was evaluated in eae-positive samples by high-throughput real-time PCR. The data presented herein demonstrated that asymptomatic children of an urban area in Brazil might be enteropathogen reservoirs, especially for STEC. |
topic |
enteric pathogens Escherichia coli pathotypes diarrheal children healthy children molecular diagnostics |
url |
https://www.frontiersin.org/article/10.3389/fcimb.2020.549919/full |
work_keys_str_mv |
AT victorrmerino prevalenceofenteropathogensandvirulencetraitsinbrazilianchildrenwithandwithoutdiarrhea AT vivianenakano prevalenceofenteropathogensandvirulencetraitsinbrazilianchildrenwithandwithoutdiarrhea AT sabinedelannoy prevalenceofenteropathogensandvirulencetraitsinbrazilianchildrenwithandwithoutdiarrhea AT patrickfach prevalenceofenteropathogensandvirulencetraitsinbrazilianchildrenwithandwithoutdiarrhea AT gabrielagfalberca prevalenceofenteropathogensandvirulencetraitsinbrazilianchildrenwithandwithoutdiarrhea AT mauriciojfarfan prevalenceofenteropathogensandvirulencetraitsinbrazilianchildrenwithandwithoutdiarrhea AT roxanemfpiazza prevalenceofenteropathogensandvirulencetraitsinbrazilianchildrenwithandwithoutdiarrhea AT mariojavilacampos prevalenceofenteropathogensandvirulencetraitsinbrazilianchildrenwithandwithoutdiarrhea |
_version_ |
1724455727975628800 |