Human Adult Periodontal Ligament-Derived Cells Integrate and Differentiate after Implantation into the Adult Mammalian Brain
Previous studies suggest that neural crest (NC)-derived stem cells may reside in NC derivatives including the human periodontal ligament (hPDL). The isolation and manipulation of autologous NC-derived cells could be an accessible source of adult neural stem cells for their use in cell replacement an...
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doaj-7f15fafe7cbc47b287a4adfd6f35a8c12020-11-25T03:01:43ZengSAGE PublishingCell Transplantation0963-68971555-38922013-11-012210.3727/096368912X657305Human Adult Periodontal Ligament-Derived Cells Integrate and Differentiate after Implantation into the Adult Mammalian BrainCarlos Bueno0Carmina Ramirez1Francisco J. Rodríguez-Lozano2Rafael Tabarés-Seisdedos3Mónica Rodenas4Jose M. Moraleda5Jonathan R. Jones6Salvador Martinez Ph.D.7 Instituto de Neurociencias de Alicante (UMH-CSIC), Sant Juan, Alicante, Spain Instituto de Neurociencias de Alicante (UMH-CSIC), Sant Juan, Alicante, Spain Cell Therapy Unit, Hospital Universitary Virgen de la Arrixaca, Faculty of Medicine, University of Murcia, Murcia, Spain Department of Medicine, University of Valencia, Centro de Investigación Biomédica en Red en el area de Salud Mental (CIBERSAM), Valencia, Spain Instituto de Neurociencias de Alicante (UMH-CSIC), Sant Juan, Alicante, Spain Cell Therapy Unit, Hospital Universitary Virgen de la Arrixaca, Faculty of Medicine, University of Murcia, Murcia, Spain Instituto de Neurociencias de Alicante (UMH-CSIC), Sant Juan, Alicante, Spain Instituto de Neurociencias de Alicante (UMH-CSIC), Sant Juan, Alicante, SpainPrevious studies suggest that neural crest (NC)-derived stem cells may reside in NC derivatives including the human periodontal ligament (hPDL). The isolation and manipulation of autologous NC-derived cells could be an accessible source of adult neural stem cells for their use in cell replacement and gene transfer to the diseased central nervous system. In this study, we examined the expression of NC markers and neural differentiation potential of hPDL-derived cells both in vitro and in vivo. In vitro we found that hPDL-derived cells expressed stem cell markers (Oct3/4, Nestin, Sox2, and Musashi-1) and a subset of NC cell markers (Slug, p75 NTR , Twist, and Sox9). hPDL-derived cells differentiated into neural-like cells based on cellular morphology and neural marker expression (TUJ1, MAP2, MAP1b, GAD65/67, GABA, NeuN, ChAT, GAT1, synaptophysin, GFAP, NG2, and O4). In vivo, hPDL-derived cells survive, migrate, and give rise to DCX + , NF-M + , GABA + , GFAP + , and NG2 + cells after grafting the adult mouse brain. Some of the grafted hPDL-derived cells were located in stem cell niches such as the ventricular epithelium and the subventricular zone of the anterolateral ventricle wall as well as in the subgranular zone of the hippocampal dentate gyrus. Thus, the hPDL contains stem cells that originate from the NC and can differentiate into neural cells. The engraftment and differentiation properties of hPDL-derived cells in the adult brain indicate that they are a potential stem cell source to be used in neuroregenerative and/or neurotrophic medicine.https://doi.org/10.3727/096368912X657305 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Carlos Bueno Carmina Ramirez Francisco J. Rodríguez-Lozano Rafael Tabarés-Seisdedos Mónica Rodenas Jose M. Moraleda Jonathan R. Jones Salvador Martinez Ph.D. |
spellingShingle |
Carlos Bueno Carmina Ramirez Francisco J. Rodríguez-Lozano Rafael Tabarés-Seisdedos Mónica Rodenas Jose M. Moraleda Jonathan R. Jones Salvador Martinez Ph.D. Human Adult Periodontal Ligament-Derived Cells Integrate and Differentiate after Implantation into the Adult Mammalian Brain Cell Transplantation |
author_facet |
Carlos Bueno Carmina Ramirez Francisco J. Rodríguez-Lozano Rafael Tabarés-Seisdedos Mónica Rodenas Jose M. Moraleda Jonathan R. Jones Salvador Martinez Ph.D. |
author_sort |
Carlos Bueno |
title |
Human Adult Periodontal Ligament-Derived Cells Integrate and Differentiate after Implantation into the Adult Mammalian Brain |
title_short |
Human Adult Periodontal Ligament-Derived Cells Integrate and Differentiate after Implantation into the Adult Mammalian Brain |
title_full |
Human Adult Periodontal Ligament-Derived Cells Integrate and Differentiate after Implantation into the Adult Mammalian Brain |
title_fullStr |
Human Adult Periodontal Ligament-Derived Cells Integrate and Differentiate after Implantation into the Adult Mammalian Brain |
title_full_unstemmed |
Human Adult Periodontal Ligament-Derived Cells Integrate and Differentiate after Implantation into the Adult Mammalian Brain |
title_sort |
human adult periodontal ligament-derived cells integrate and differentiate after implantation into the adult mammalian brain |
publisher |
SAGE Publishing |
series |
Cell Transplantation |
issn |
0963-6897 1555-3892 |
publishDate |
2013-11-01 |
description |
Previous studies suggest that neural crest (NC)-derived stem cells may reside in NC derivatives including the human periodontal ligament (hPDL). The isolation and manipulation of autologous NC-derived cells could be an accessible source of adult neural stem cells for their use in cell replacement and gene transfer to the diseased central nervous system. In this study, we examined the expression of NC markers and neural differentiation potential of hPDL-derived cells both in vitro and in vivo. In vitro we found that hPDL-derived cells expressed stem cell markers (Oct3/4, Nestin, Sox2, and Musashi-1) and a subset of NC cell markers (Slug, p75 NTR , Twist, and Sox9). hPDL-derived cells differentiated into neural-like cells based on cellular morphology and neural marker expression (TUJ1, MAP2, MAP1b, GAD65/67, GABA, NeuN, ChAT, GAT1, synaptophysin, GFAP, NG2, and O4). In vivo, hPDL-derived cells survive, migrate, and give rise to DCX + , NF-M + , GABA + , GFAP + , and NG2 + cells after grafting the adult mouse brain. Some of the grafted hPDL-derived cells were located in stem cell niches such as the ventricular epithelium and the subventricular zone of the anterolateral ventricle wall as well as in the subgranular zone of the hippocampal dentate gyrus. Thus, the hPDL contains stem cells that originate from the NC and can differentiate into neural cells. The engraftment and differentiation properties of hPDL-derived cells in the adult brain indicate that they are a potential stem cell source to be used in neuroregenerative and/or neurotrophic medicine. |
url |
https://doi.org/10.3727/096368912X657305 |
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