Effects of Soil and Substrate Cultivation on Lettuce Rhizosphere Bacterial Community

• Main Authors: LIANG Yun, ZHAO Li, HUANG Dan-feng
• Format: Article
• Language: zho
• Published: Agro-Environmental Protection Institute, Ministry of Agriculture 2017-01-01
• Series: Journal of Agricultural Resources and Environment
• Subjects: soil cultivation, substrate cultivation, rhizosphere bacterial community, PCR-DGGE
• Online Access: http://www.aed.org.cn/nyzyyhjxb/html/2017/1/20170111.htm
• ISSN: 2095-6819; 2095-6819

Rhizosphere bacterial community can promote the nutrition absorption of plant root, which result in the upgrade of plant quality. Cultivation system has effect on rhizosphere bacterial community. Four treatments were set to investigate the effects of two different cultivation systems, soil and substrate systems, for two varieties of lettuce, Shengxuan NO.5 and cv. Lollo Rossca.(two cultivation systems &#215; two varieties). Each treatment had three pots as samples with 10 lettuce plants for each pot. After 30 days of transplanting, five plants of each pot were randomly selected, and rhizosphere soil or substrate was sampled. Real-time PCR and PCR-DGGE were implied to analyze the characteristics of rhizosphere bacterial community in each treatment. Real-Time PCR detection showed that the number of the population of rhizosphere bacteria in substrate system was significantly higher than that of soil system(P<0.05). PCR-DGGE profiles revealed that the diversity of substrate system was significantly higher than that of soil system. As for Shenxuan NO.5, the Shannon-Wiener index(H), Simpson index(D)and Pielou evenness index(E)of substrate system were significantly higher than that of soil system(P<0.05), and for cv.Lollo Rossca, index H of substrate system were significantly higher than that of soil system(P<0.05). RDA revealed that soil and substrate systems had different bacterial communities, and pH and nitrate nitrogen were two main factors that determining the community structure. In addition, water content, C/N, and available phosphorus were positively correlated with the development of bacterial community. Overall, soil and substrate cultivation systems had different rhizosphere bacterial community, and the quantity and diversity were higher in substrate system due to the physiochemical difference.