Sialic Acid Expression in the Mosquito Aedes aegypti and Its Possible Role in Dengue Virus-Vector Interactions
Dengue fever (DF) is the most prevalent arthropod-borne viral disease which affects humans. DF is caused by the four dengue virus (DENV) serotypes, which are transmitted to the host by the mosquito Aedes aegypti that has key roles in DENV infection, replication, and viral transmission (vector compe...
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doaj-7f1a0ff05513445b93fda4250ae9b3442020-11-25T00:11:00ZengHindawi LimitedBioMed Research International2314-61332314-61412015-01-01201510.1155/2015/504187504187Sialic Acid Expression in the Mosquito Aedes aegypti and Its Possible Role in Dengue Virus-Vector InteractionsJorge Cime-Castillo0Philippe Delannoy1Guillermo Mendoza-Hernández2Verónica Monroy-Martínez3Anne Harduin-Lepers4Humberto Lanz-Mendoza5Fidel de la Cruz Hernández-Hernández6Edgar Zenteno7Carlos Cabello-Gutiérrez8Blanca H. Ruiz-Ordaz9Molecular Biology and Biotechnology Department, Biomedical Research Institute, National University of México (UNAM), 04510 México City, MexicoStructural and Functional Glycobiology Unit, UMR 8576 CNRS, University of Sciences and Technologies of Lille, 59655 Villeneuve d’Ascq, FranceBiochemistry Department, Faculty of Medicine, UNAM, 04510 México City, MexicoMolecular Biology and Biotechnology Department, Biomedical Research Institute, National University of México (UNAM), 04510 México City, MexicoStructural and Functional Glycobiology Unit, UMR 8576 CNRS, University of Sciences and Technologies of Lille, 59655 Villeneuve d’Ascq, FranceCISEI, National Institute of Public Health, 62100 Cuernavaca, MOR, MexicoInfectomics and Molecular Pathogenesis Department, CINVESTAV-IPN, 07360 México City, MexicoBiochemistry Department, Faculty of Medicine, UNAM, 04510 México City, MexicoVirology Department, National Respiratory Institute (INER), 14050 México City, MexicoMolecular Biology and Biotechnology Department, Biomedical Research Institute, National University of México (UNAM), 04510 México City, MexicoDengue fever (DF) is the most prevalent arthropod-borne viral disease which affects humans. DF is caused by the four dengue virus (DENV) serotypes, which are transmitted to the host by the mosquito Aedes aegypti that has key roles in DENV infection, replication, and viral transmission (vector competence). Mosquito saliva also plays an important role during DENV transmission. In this study, we detected the presence of sialic acid (Sia) in Aedes aegypti tissues, which may have an important role during DENV-vector competence. We also identified genome sequences encoding enzymes involved in Sia pathways. The cDNA for Aedes aegypti CMP-Sia synthase (CSAS) was amplified, cloned, and functionally evaluated via the complementation of LEC29.Lec32 CSAS-deficient CHO cells. AedesCSAS-transfected LEC29.Lec32 cells were able to express Sia moieties on the cell surface. Sequences related to α-2,6-sialyltransferase were detected in the Aedes aegypti genome. Likewise, we identified Sia-α-2,6-DENV interactions in different mosquito tissues. In addition, we evaluated the possible role of sialylated molecules in a salivary gland extract during DENV internalization in mammalian cells. The knowledge of early DENV-host interactions could facilitate a better understanding of viral tropism and pathogenesis to allow the development of new strategies for controlling DENV transmission.http://dx.doi.org/10.1155/2015/504187 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jorge Cime-Castillo Philippe Delannoy Guillermo Mendoza-Hernández Verónica Monroy-Martínez Anne Harduin-Lepers Humberto Lanz-Mendoza Fidel de la Cruz Hernández-Hernández Edgar Zenteno Carlos Cabello-Gutiérrez Blanca H. Ruiz-Ordaz |
spellingShingle |
Jorge Cime-Castillo Philippe Delannoy Guillermo Mendoza-Hernández Verónica Monroy-Martínez Anne Harduin-Lepers Humberto Lanz-Mendoza Fidel de la Cruz Hernández-Hernández Edgar Zenteno Carlos Cabello-Gutiérrez Blanca H. Ruiz-Ordaz Sialic Acid Expression in the Mosquito Aedes aegypti and Its Possible Role in Dengue Virus-Vector Interactions BioMed Research International |
author_facet |
Jorge Cime-Castillo Philippe Delannoy Guillermo Mendoza-Hernández Verónica Monroy-Martínez Anne Harduin-Lepers Humberto Lanz-Mendoza Fidel de la Cruz Hernández-Hernández Edgar Zenteno Carlos Cabello-Gutiérrez Blanca H. Ruiz-Ordaz |
author_sort |
Jorge Cime-Castillo |
title |
Sialic Acid Expression in the Mosquito Aedes aegypti and Its Possible Role in Dengue Virus-Vector Interactions |
title_short |
Sialic Acid Expression in the Mosquito Aedes aegypti and Its Possible Role in Dengue Virus-Vector Interactions |
title_full |
Sialic Acid Expression in the Mosquito Aedes aegypti and Its Possible Role in Dengue Virus-Vector Interactions |
title_fullStr |
Sialic Acid Expression in the Mosquito Aedes aegypti and Its Possible Role in Dengue Virus-Vector Interactions |
title_full_unstemmed |
Sialic Acid Expression in the Mosquito Aedes aegypti and Its Possible Role in Dengue Virus-Vector Interactions |
title_sort |
sialic acid expression in the mosquito aedes aegypti and its possible role in dengue virus-vector interactions |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2015-01-01 |
description |
Dengue fever (DF) is the most prevalent arthropod-borne viral disease which affects humans. DF is caused by the four dengue virus (DENV) serotypes, which are transmitted to the host by the mosquito Aedes aegypti that has key roles in DENV infection, replication, and viral transmission (vector competence). Mosquito saliva also plays an important role during DENV transmission. In this study, we detected the presence of sialic acid (Sia) in Aedes aegypti tissues, which may have an important role during DENV-vector competence. We also identified genome sequences encoding enzymes involved in Sia pathways. The cDNA for Aedes aegypti CMP-Sia synthase (CSAS) was amplified, cloned, and functionally evaluated via the complementation of LEC29.Lec32 CSAS-deficient CHO cells. AedesCSAS-transfected LEC29.Lec32 cells were able to express Sia moieties on the cell surface. Sequences related to α-2,6-sialyltransferase were detected in the Aedes aegypti genome. Likewise, we identified Sia-α-2,6-DENV interactions in different mosquito tissues. In addition, we evaluated the possible role of sialylated molecules in a salivary gland extract during DENV internalization in mammalian cells. The knowledge of early DENV-host interactions could facilitate a better understanding of viral tropism and pathogenesis to allow the development of new strategies for controlling DENV transmission. |
url |
http://dx.doi.org/10.1155/2015/504187 |
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