Aldosterone Rapidly Enhances Levels of the Striatin and Caveolin-1 Proteins in Rat Kidney: The Role of the Mineralocorticoid Receptor
BackgroundStriatin and caveolin-1 (cav-1) are scaffolding/regulating proteins that are associated with salt-sensitive high blood pressure and promote renal sodium and water reabsorption, respectively. The mineralocorticoid receptor (MR) interacts with striatin and cav-1, while aldosterone increases...
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doaj-7f2986eae1eb479bb74ac0786e8478e52020-11-25T02:06:33ZengAcademya Publishing Co.Endocrinology and Metabolism2093-596X2093-59782019-09-0134329130110.3803/EnM.2019.34.3.291Aldosterone Rapidly Enhances Levels of the Striatin and Caveolin-1 Proteins in Rat Kidney: The Role of the Mineralocorticoid ReceptorKevalin Inthachart0Krissanapong Manotham1Somchai Eiam-Ong2Somchit Eiam-Ong3Interdepartment of Physiology, Chulalongkorn University Graduate School, Bangkok, .ThailandDepartment of Medicine, Lerdsin General Hospital, Bangkok, .ThailandDivision of Nephrology, Department of Medicine, Chulalongkorn University Faculty of Medicine, Bangkok, .ThailandDepartment of Physiology, Chulalongkorn University Faculty of Medicine, Bangkok, .ThailandBackgroundStriatin and caveolin-1 (cav-1) are scaffolding/regulating proteins that are associated with salt-sensitive high blood pressure and promote renal sodium and water reabsorption, respectively. The mineralocorticoid receptor (MR) interacts with striatin and cav-1, while aldosterone increases striatin and cav-1 levels. However, no in vivo data have been reported for the levels of these proteins in the kidney.MethodsMale Wistar rats were intraperitoneally injected with normal saline solution, aldosterone alone (Aldo: 150 µg/kg body weight), or aldosterone after pretreatment with eplerenone, an MR blocker, 30 minutes before the aldosterone injection (eplerenone [Ep.]+Aldo). Thirty minutes after the aldosterone injection, the amount and localization of striatin and cav-1 were determined by Western blot analysis and immunohistochemistry, respectively.ResultsAldosterone increased striatin levels by 150% (P<0.05), and cav-1 levels by 200% (P<0.001). Eplerenone had no significant effect on striatin levels, but partially blocked the aldosterone-induced increase in cav-1 levels. Aldosterone stimulated striatin and cav-1 immunoreactivity in both the cortex and medulla. Eplerenone reduced cav-1 immunostaining in both areas; however, striatin intensity was reduced in the cortex, but increased in the medulla.ConclusionThis is the first in vivo study demonstrating that aldosterone rapidly enhances renal levels of striatin and cav-1. Aldosterone increases striatin levels via an MR-independent pathway, whereas cav-1 is partially regulated through MR.https://e-enm.org/Synapse/Data/PDFData/2008ENM/enm-34-291.pdfaldosteronerapid actionstriatincaveolin 1protein levelsrat kidney |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kevalin Inthachart Krissanapong Manotham Somchai Eiam-Ong Somchit Eiam-Ong |
spellingShingle |
Kevalin Inthachart Krissanapong Manotham Somchai Eiam-Ong Somchit Eiam-Ong Aldosterone Rapidly Enhances Levels of the Striatin and Caveolin-1 Proteins in Rat Kidney: The Role of the Mineralocorticoid Receptor Endocrinology and Metabolism aldosterone rapid action striatin caveolin 1 protein levels rat kidney |
author_facet |
Kevalin Inthachart Krissanapong Manotham Somchai Eiam-Ong Somchit Eiam-Ong |
author_sort |
Kevalin Inthachart |
title |
Aldosterone Rapidly Enhances Levels of the Striatin and Caveolin-1 Proteins in Rat Kidney: The Role of the Mineralocorticoid Receptor |
title_short |
Aldosterone Rapidly Enhances Levels of the Striatin and Caveolin-1 Proteins in Rat Kidney: The Role of the Mineralocorticoid Receptor |
title_full |
Aldosterone Rapidly Enhances Levels of the Striatin and Caveolin-1 Proteins in Rat Kidney: The Role of the Mineralocorticoid Receptor |
title_fullStr |
Aldosterone Rapidly Enhances Levels of the Striatin and Caveolin-1 Proteins in Rat Kidney: The Role of the Mineralocorticoid Receptor |
title_full_unstemmed |
Aldosterone Rapidly Enhances Levels of the Striatin and Caveolin-1 Proteins in Rat Kidney: The Role of the Mineralocorticoid Receptor |
title_sort |
aldosterone rapidly enhances levels of the striatin and caveolin-1 proteins in rat kidney: the role of the mineralocorticoid receptor |
publisher |
Academya Publishing Co. |
series |
Endocrinology and Metabolism |
issn |
2093-596X 2093-5978 |
publishDate |
2019-09-01 |
description |
BackgroundStriatin and caveolin-1 (cav-1) are scaffolding/regulating proteins that are associated with salt-sensitive high blood pressure and promote renal sodium and water reabsorption, respectively. The mineralocorticoid receptor (MR) interacts with striatin and cav-1, while aldosterone increases striatin and cav-1 levels. However, no in vivo data have been reported for the levels of these proteins in the kidney.MethodsMale Wistar rats were intraperitoneally injected with normal saline solution, aldosterone alone (Aldo: 150 µg/kg body weight), or aldosterone after pretreatment with eplerenone, an MR blocker, 30 minutes before the aldosterone injection (eplerenone [Ep.]+Aldo). Thirty minutes after the aldosterone injection, the amount and localization of striatin and cav-1 were determined by Western blot analysis and immunohistochemistry, respectively.ResultsAldosterone increased striatin levels by 150% (P<0.05), and cav-1 levels by 200% (P<0.001). Eplerenone had no significant effect on striatin levels, but partially blocked the aldosterone-induced increase in cav-1 levels. Aldosterone stimulated striatin and cav-1 immunoreactivity in both the cortex and medulla. Eplerenone reduced cav-1 immunostaining in both areas; however, striatin intensity was reduced in the cortex, but increased in the medulla.ConclusionThis is the first in vivo study demonstrating that aldosterone rapidly enhances renal levels of striatin and cav-1. Aldosterone increases striatin levels via an MR-independent pathway, whereas cav-1 is partially regulated through MR. |
topic |
aldosterone rapid action striatin caveolin 1 protein levels rat kidney |
url |
https://e-enm.org/Synapse/Data/PDFData/2008ENM/enm-34-291.pdf |
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