Prediction of antigenic epitopes for coat protein of Potato virus Y, Egypt
Isolated Potato virus Y Egypt (PVY- EG) obtained from naturally infected potato plants in El-Sharkia, Egypt, was identified by biological, molecular and serological assays. The aims of this study were to predict the antigenic determinants (epitopes) of the isolated PVY using immunoinformatics; to...
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Ain Shams University
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doaj-7f2c73b4e37d4f8ba7dd0eba1dea4a9b2021-04-16T22:14:58ZengAin Shams UniversityNovel Research in Microbiology Journal2537-02862537-02942019-04-013229731310.21608/nrmj.2019.30610Prediction of antigenic epitopes for coat protein of Potato virus Y, EgyptSeham Abdel-Shafi0Ghaly Mohamed Farouk1Mohamed Taha 2Khalid El-Dougdoug3Department of Botany and Microbiology, Faculty of Science, Zagazig University, Zagazig, EgyptDepartment of Botany and Microbiology, Faculty of Science, Zagazig University, Zagazig, EgyptDepartment of Botany and Microbiology, Faculty of Science, Zagazig University, Zagazig, EgyptDepartment of Microbiology, Faculty of Agriculture, Ain Shams University, EgyptIsolated Potato virus Y Egypt (PVY- EG) obtained from naturally infected potato plants in El-Sharkia, Egypt, was identified by biological, molecular and serological assays. The aims of this study were to predict the antigenic determinants (epitopes) of the isolated PVY using immunoinformatics; to prepare antibodies then apply them for PVY screening in potato plants in the field, as well as comparison with antibodies against coat protein. The amino acid sequence of PVY isolate was expected by DNAStar protean system; in which four parameters for epitope prediction including antigenicity, surface probability, hydrophobicity and secondary structure were used. These models were applied to PVY as a case study to predict immunogenic peptides for antibodies production. The most hydrophilic regions of PVY-coat protein (PVY- CP) were; 36-40, 46-47, 67-74, 96-98, 130-133, 164-166 and 198-200, which showed high hydrophobicity of this protein. This indicated that this protein was one of the best candidates to be immunogenic, and capable of producing antibodies that cross react with PVY. The peptide was chemically synthesized and injected into a rabbit. Obtained antibodies were evaluated using Double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), and Immune Dot-Blot assay. These antibodies positively reacted against PVY infected potato tissues. https://nrmj.journals.ekb.eg/article_30610_c8852e0e9fbf9037954f6e15220b8a22.pdfpotyviruspotatoimmunoinformaticselisapabs |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Seham Abdel-Shafi Ghaly Mohamed Farouk Mohamed Taha Khalid El-Dougdoug |
spellingShingle |
Seham Abdel-Shafi Ghaly Mohamed Farouk Mohamed Taha Khalid El-Dougdoug Prediction of antigenic epitopes for coat protein of Potato virus Y, Egypt Novel Research in Microbiology Journal potyvirus potato immunoinformatics elisa pabs |
author_facet |
Seham Abdel-Shafi Ghaly Mohamed Farouk Mohamed Taha Khalid El-Dougdoug |
author_sort |
Seham Abdel-Shafi |
title |
Prediction of antigenic epitopes for coat protein of Potato virus Y, Egypt |
title_short |
Prediction of antigenic epitopes for coat protein of Potato virus Y, Egypt |
title_full |
Prediction of antigenic epitopes for coat protein of Potato virus Y, Egypt |
title_fullStr |
Prediction of antigenic epitopes for coat protein of Potato virus Y, Egypt |
title_full_unstemmed |
Prediction of antigenic epitopes for coat protein of Potato virus Y, Egypt |
title_sort |
prediction of antigenic epitopes for coat protein of potato virus y, egypt |
publisher |
Ain Shams University |
series |
Novel Research in Microbiology Journal |
issn |
2537-0286 2537-0294 |
publishDate |
2019-04-01 |
description |
Isolated Potato virus Y Egypt (PVY- EG) obtained from naturally infected potato plants in El-Sharkia, Egypt,
was identified by biological, molecular and serological assays. The aims of this study were to predict the antigenic
determinants (epitopes) of the isolated PVY using immunoinformatics; to prepare antibodies then apply them for
PVY screening in potato plants in the field, as well as comparison with antibodies against coat protein. The amino
acid sequence of PVY isolate was expected by DNAStar protean system; in which four parameters for epitope
prediction including antigenicity, surface probability, hydrophobicity and secondary structure were used. These
models were applied to PVY as a case study to predict immunogenic peptides for antibodies production. The most
hydrophilic regions of PVY-coat protein (PVY- CP) were; 36-40, 46-47, 67-74, 96-98, 130-133, 164-166 and
198-200, which showed high hydrophobicity of this protein. This indicated that this protein was one of the best
candidates to be immunogenic, and capable of producing antibodies that cross react with PVY. The peptide was
chemically synthesized and injected into a rabbit. Obtained antibodies were evaluated using Double antibody
sandwich enzyme-linked immunosorbent assay (DAS-ELISA), and Immune Dot-Blot assay. These antibodies
positively reacted against PVY infected potato tissues. |
topic |
potyvirus potato immunoinformatics elisa pabs |
url |
https://nrmj.journals.ekb.eg/article_30610_c8852e0e9fbf9037954f6e15220b8a22.pdf |
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