Genetic Diversity, Nitrogen Fixation, and Water Use Efficiency in a Panel of Honduran Common Bean (<em>Phaseolus vulgaris</em> L.) Landraces and Modern Genotypes
Common bean (<i>Phaseolus vulgaris </i>L.) provides critical nutrition and a livelihood for millions of smallholder farmers worldwide. Beans engage in symbiotic nitrogen fixation (SNF) with Rhizobia. Honduran hillside farmers farm marginal land and utilize few production inputs; therefor...
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doaj-7f306a3bce8a41348fcb9855300308012020-11-25T03:56:55ZengMDPI AGPlants2223-77472020-09-0191238123810.3390/plants9091238Genetic Diversity, Nitrogen Fixation, and Water Use Efficiency in a Panel of Honduran Common Bean (<em>Phaseolus vulgaris</em> L.) Landraces and Modern GenotypesJennifer Wilker0Sally Humphries1Juan Carlos Rosas-Sotomayor2Marvin Gómez Cerna3Davoud Torkamaneh4Michelle Edwards5Alireza Navabi6K. Peter Pauls7Department of Plant Agriculture, University of Guelph, Guelph, ON N1G 2W1, CanadaDepartment of Sociology and Anthropology, University of Guelph, Guelph, ON N1G 2W1, CanadaDepartamento de Ciencia y Producción Agropecuaria, Escuela Agrícola Panamericana, Zamorano, Tegucigalpa 11101, HondurasFundación para la Investigación Participativa con Agricultores de Honduras, La Ceiba, Atlántida 561, HondurasDepartment of Plant Agriculture, University of Guelph, Guelph, ON N1G 2W1, CanadaDepartment of Plant Agriculture, University of Guelph, Guelph, ON N1G 2W1, CanadaDepartment of Plant Agriculture, University of Guelph, Guelph, ON N1G 2W1, CanadaDepartment of Plant Agriculture, University of Guelph, Guelph, ON N1G 2W1, CanadaCommon bean (<i>Phaseolus vulgaris </i>L.) provides critical nutrition and a livelihood for millions of smallholder farmers worldwide. Beans engage in symbiotic nitrogen fixation (SNF) with Rhizobia. Honduran hillside farmers farm marginal land and utilize few production inputs; therefore, bean varieties with high SNF capacity and environmental resiliency would be of benefit to them. We explored the diversity for SNF, agronomic traits, and water use efficiency (WUE) among 70 Honduran landrace, participatory bred (PPB), and conventionally bred bean varieties (HON panel) and 6 North American check varieties in 3 low-N field trials in Ontario, Canada and Honduras. Genetic diversity was measured with a 6K single nucleotide polymorphism (SNP) array, and phenotyping for agronomic, SNF, and WUE traits was carried out. STRUCTURE analysis revealed two subpopulations with admixture between the subpopulations. Nucleotide diversity was greater in the landraces than the PPB varieties across the genome, and multiple genomic regions were identified where population genetic differentiation between the landraces and PPB varieties was evident. Significant differences were found between varieties and breeding categories for agronomic traits, SNF, and WUE. Landraces had above average SNF capacity, conventional varieties showed higher yields, and PPB varieties performed well for WUE. Varieties with the best SNF capacity could be used in further participatory breeding efforts.https://www.mdpi.com/2223-7747/9/9/1238nitrogen fixationsymbiosisbeanlandracePPBparticipatory breeding |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jennifer Wilker Sally Humphries Juan Carlos Rosas-Sotomayor Marvin Gómez Cerna Davoud Torkamaneh Michelle Edwards Alireza Navabi K. Peter Pauls |
spellingShingle |
Jennifer Wilker Sally Humphries Juan Carlos Rosas-Sotomayor Marvin Gómez Cerna Davoud Torkamaneh Michelle Edwards Alireza Navabi K. Peter Pauls Genetic Diversity, Nitrogen Fixation, and Water Use Efficiency in a Panel of Honduran Common Bean (<em>Phaseolus vulgaris</em> L.) Landraces and Modern Genotypes Plants nitrogen fixation symbiosis bean landrace PPB participatory breeding |
author_facet |
Jennifer Wilker Sally Humphries Juan Carlos Rosas-Sotomayor Marvin Gómez Cerna Davoud Torkamaneh Michelle Edwards Alireza Navabi K. Peter Pauls |
author_sort |
Jennifer Wilker |
title |
Genetic Diversity, Nitrogen Fixation, and Water Use Efficiency in a Panel of Honduran Common Bean (<em>Phaseolus vulgaris</em> L.) Landraces and Modern Genotypes |
title_short |
Genetic Diversity, Nitrogen Fixation, and Water Use Efficiency in a Panel of Honduran Common Bean (<em>Phaseolus vulgaris</em> L.) Landraces and Modern Genotypes |
title_full |
Genetic Diversity, Nitrogen Fixation, and Water Use Efficiency in a Panel of Honduran Common Bean (<em>Phaseolus vulgaris</em> L.) Landraces and Modern Genotypes |
title_fullStr |
Genetic Diversity, Nitrogen Fixation, and Water Use Efficiency in a Panel of Honduran Common Bean (<em>Phaseolus vulgaris</em> L.) Landraces and Modern Genotypes |
title_full_unstemmed |
Genetic Diversity, Nitrogen Fixation, and Water Use Efficiency in a Panel of Honduran Common Bean (<em>Phaseolus vulgaris</em> L.) Landraces and Modern Genotypes |
title_sort |
genetic diversity, nitrogen fixation, and water use efficiency in a panel of honduran common bean (<em>phaseolus vulgaris</em> l.) landraces and modern genotypes |
publisher |
MDPI AG |
series |
Plants |
issn |
2223-7747 |
publishDate |
2020-09-01 |
description |
Common bean (<i>Phaseolus vulgaris </i>L.) provides critical nutrition and a livelihood for millions of smallholder farmers worldwide. Beans engage in symbiotic nitrogen fixation (SNF) with Rhizobia. Honduran hillside farmers farm marginal land and utilize few production inputs; therefore, bean varieties with high SNF capacity and environmental resiliency would be of benefit to them. We explored the diversity for SNF, agronomic traits, and water use efficiency (WUE) among 70 Honduran landrace, participatory bred (PPB), and conventionally bred bean varieties (HON panel) and 6 North American check varieties in 3 low-N field trials in Ontario, Canada and Honduras. Genetic diversity was measured with a 6K single nucleotide polymorphism (SNP) array, and phenotyping for agronomic, SNF, and WUE traits was carried out. STRUCTURE analysis revealed two subpopulations with admixture between the subpopulations. Nucleotide diversity was greater in the landraces than the PPB varieties across the genome, and multiple genomic regions were identified where population genetic differentiation between the landraces and PPB varieties was evident. Significant differences were found between varieties and breeding categories for agronomic traits, SNF, and WUE. Landraces had above average SNF capacity, conventional varieties showed higher yields, and PPB varieties performed well for WUE. Varieties with the best SNF capacity could be used in further participatory breeding efforts. |
topic |
nitrogen fixation symbiosis bean landrace PPB participatory breeding |
url |
https://www.mdpi.com/2223-7747/9/9/1238 |
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