First report of a newly-described lungworm, Dictyocaulus cervi (Nematoda: Trichostrongyloidea), in moose (Alces alces) in central Europe
Lungworms from the genus Dictyocaulus are the causative agents of verminous pneumonia in domestic and wild ungulates. Recently, in 2017, a new species was isolated from red deer and described as Dictyocaulus cervi; however, little is known about its epidemiology and pathogenicity in other cervids. T...
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doaj-7f3550874b2740cc8224f091109b1e712020-12-19T05:06:40ZengElsevierInternational Journal for Parasitology: Parasites and Wildlife2213-22442020-12-0113275282First report of a newly-described lungworm, Dictyocaulus cervi (Nematoda: Trichostrongyloidea), in moose (Alces alces) in central EuropeKatarzyna Filip-Hutsch0Aleksander W. Demiaszkiewicz1Anita Chęcińska2Tomasz Hutsch3Michał Czopowicz4Anna M. Pyziel5Witold Stefański Institute of Parasitology PAS, Twarda 51/55, 00-818, Warsaw, Poland; Corresponding author.Witold Stefański Institute of Parasitology PAS, Twarda 51/55, 00-818, Warsaw, PolandVeterinary Diagnostic Laboratory ALAB bioscience, Krucza 13, 05-090, Rybie, Poland; Molecular Biology Unit, Mossakowski Medical Research Centre PAS, A. Pawińskiego 5, 02-106, Warsaw, PolandVeterinary Diagnostic Laboratory ALAB bioscience, Krucza 13, 05-090, Rybie, PolandDivision of Veterinary Epidemiology and Economics, Institute of Veterinary Medicine, Warsaw University of Life Sciences–SGGW (WULS), Nowoursynowska 159c, 02-776, Warsaw, PolandDepartment of Food Hygiene and Public Health Protection, Institute of Veterinary Medicine, Warsaw University of Life Sciences – SGGW (WULS), Nowoursynowska 159, 02-776, Warsaw, PolandLungworms from the genus Dictyocaulus are the causative agents of verminous pneumonia in domestic and wild ungulates. Recently, in 2017, a new species was isolated from red deer and described as Dictyocaulus cervi; however, little is known about its epidemiology and pathogenicity in other cervids. The aim of our study was to determine the extent of infection with Dictyocaulus nematodes in the moose population in Poland.Parasitological necropsies were performed in 18 moose and 249 faecal samples were analysed. A combination of multiplex PCR and analysis of the partial SSU, cox1 and cyt B regions revealed the presence of D. cervi infection in two of the necropsied moose. Histopathological examinations revealed changes, including multiple cross sections of larvae of nematodes in alveoli, massive pulmonary fibrosis, mononuclear cell infiltration and diffuse alveolar damage in the lungs of four animals. The lesions were more pronounced when adult Dictyocaulus nematodes were present in the bronchi and bronchioles. Some of the observed pathological changes could be attributed to co-infection by nematodes of the Protostrongylidae, whose larvae were found in all four animals with lung pathologies. In the faeces, Dictyocaulus sp. larvae only occurred together with Protostrongylidae larvae; in addition, higher numbers of Protostrongylidae larvae were excreted in the faeces of animals with dictyocaulosis.The present study is the first report of the presence of D. cervi in moose, and demonstrates the value of multiplex PCR in the identification of Dictyocaulus nematodes. Our findings indicate that co-infections with multiple species of lung nematodes in moose may be commonplace, and this should be considered as a factor aggravating the course of parasitosis.http://www.sciencedirect.com/science/article/pii/S2213224420301085Alces alcesDictyocaulosisProtostrongylidaeHistopathologyFaecal analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Katarzyna Filip-Hutsch Aleksander W. Demiaszkiewicz Anita Chęcińska Tomasz Hutsch Michał Czopowicz Anna M. Pyziel |
spellingShingle |
Katarzyna Filip-Hutsch Aleksander W. Demiaszkiewicz Anita Chęcińska Tomasz Hutsch Michał Czopowicz Anna M. Pyziel First report of a newly-described lungworm, Dictyocaulus cervi (Nematoda: Trichostrongyloidea), in moose (Alces alces) in central Europe International Journal for Parasitology: Parasites and Wildlife Alces alces Dictyocaulosis Protostrongylidae Histopathology Faecal analysis |
author_facet |
Katarzyna Filip-Hutsch Aleksander W. Demiaszkiewicz Anita Chęcińska Tomasz Hutsch Michał Czopowicz Anna M. Pyziel |
author_sort |
Katarzyna Filip-Hutsch |
title |
First report of a newly-described lungworm, Dictyocaulus cervi (Nematoda: Trichostrongyloidea), in moose (Alces alces) in central Europe |
title_short |
First report of a newly-described lungworm, Dictyocaulus cervi (Nematoda: Trichostrongyloidea), in moose (Alces alces) in central Europe |
title_full |
First report of a newly-described lungworm, Dictyocaulus cervi (Nematoda: Trichostrongyloidea), in moose (Alces alces) in central Europe |
title_fullStr |
First report of a newly-described lungworm, Dictyocaulus cervi (Nematoda: Trichostrongyloidea), in moose (Alces alces) in central Europe |
title_full_unstemmed |
First report of a newly-described lungworm, Dictyocaulus cervi (Nematoda: Trichostrongyloidea), in moose (Alces alces) in central Europe |
title_sort |
first report of a newly-described lungworm, dictyocaulus cervi (nematoda: trichostrongyloidea), in moose (alces alces) in central europe |
publisher |
Elsevier |
series |
International Journal for Parasitology: Parasites and Wildlife |
issn |
2213-2244 |
publishDate |
2020-12-01 |
description |
Lungworms from the genus Dictyocaulus are the causative agents of verminous pneumonia in domestic and wild ungulates. Recently, in 2017, a new species was isolated from red deer and described as Dictyocaulus cervi; however, little is known about its epidemiology and pathogenicity in other cervids. The aim of our study was to determine the extent of infection with Dictyocaulus nematodes in the moose population in Poland.Parasitological necropsies were performed in 18 moose and 249 faecal samples were analysed. A combination of multiplex PCR and analysis of the partial SSU, cox1 and cyt B regions revealed the presence of D. cervi infection in two of the necropsied moose. Histopathological examinations revealed changes, including multiple cross sections of larvae of nematodes in alveoli, massive pulmonary fibrosis, mononuclear cell infiltration and diffuse alveolar damage in the lungs of four animals. The lesions were more pronounced when adult Dictyocaulus nematodes were present in the bronchi and bronchioles. Some of the observed pathological changes could be attributed to co-infection by nematodes of the Protostrongylidae, whose larvae were found in all four animals with lung pathologies. In the faeces, Dictyocaulus sp. larvae only occurred together with Protostrongylidae larvae; in addition, higher numbers of Protostrongylidae larvae were excreted in the faeces of animals with dictyocaulosis.The present study is the first report of the presence of D. cervi in moose, and demonstrates the value of multiplex PCR in the identification of Dictyocaulus nematodes. Our findings indicate that co-infections with multiple species of lung nematodes in moose may be commonplace, and this should be considered as a factor aggravating the course of parasitosis. |
topic |
Alces alces Dictyocaulosis Protostrongylidae Histopathology Faecal analysis |
url |
http://www.sciencedirect.com/science/article/pii/S2213224420301085 |
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