Aflatoxin M1 detection by ELISA in raw and processed milk in Bangladesh
An analysis was accomplished to get information regarding presence of highly toxic and carcinogenic aflatoxin M1 (AFM1) in raw and processed samples of milk applying ELISA (enzyme linked immunosorbent assay). Investigation of a set of 100 samples (n=100) taken from different regional small-scale far...
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doaj-7f512f60304c4f68819eefe7a13706222020-12-25T05:10:23ZengElsevierToxicology Reports2214-75002020-01-01713391343Aflatoxin M1 detection by ELISA in raw and processed milk in BangladeshNourin Tarannum0Meher Nigad Nipa1Suvra Das2Sahana Parveen3Institute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka, BangladeshInstitute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka, BangladeshInstitute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka, BangladeshCorresponding author.; Institute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka, BangladeshAn analysis was accomplished to get information regarding presence of highly toxic and carcinogenic aflatoxin M1 (AFM1) in raw and processed samples of milk applying ELISA (enzyme linked immunosorbent assay). Investigation of a set of 100 samples (n=100) taken from different regional small-scale farms as well as grocery stores of Bangladesh containing three groups of milk including raw (n=50), pasteurized (n=25) and UHT (n=25), exhibited in total 53% AFM1 contamination where 70% contamination was found in raw milk ranging from 22.79−1489.28 ng/kg (mean value 699.07 ng/kg), 52% in pasteurized milk ranging from 18.11−672.18 ng/kg (mean value 99.77 ng/kg) and 20% in UHT milk ranging from 25.07−48.95 ng/kg (mean value 35.46 ng/kg). Among all the positive samples, 75% contaminated samples were above the European Communities prescribed limits (50 ng/kg) while having 25% samples still below this limit whereas 43% samples crossed the accepted limit of US regulations/Codex Alimentarius Commission regulations (500 ng/kg). Thus the findings of this study may lead to increase awareness regarding health impact of aflatoxin M1 and implementation of strict regulations by law enforcement bureaus of Bangladesh.http://www.sciencedirect.com/science/article/pii/S2214750020304066AflatoxinAflatoxin M1Aflatoxin B1ELISAMilk |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nourin Tarannum Meher Nigad Nipa Suvra Das Sahana Parveen |
spellingShingle |
Nourin Tarannum Meher Nigad Nipa Suvra Das Sahana Parveen Aflatoxin M1 detection by ELISA in raw and processed milk in Bangladesh Toxicology Reports Aflatoxin Aflatoxin M1 Aflatoxin B1 ELISA Milk |
author_facet |
Nourin Tarannum Meher Nigad Nipa Suvra Das Sahana Parveen |
author_sort |
Nourin Tarannum |
title |
Aflatoxin M1 detection by ELISA in raw and processed milk in Bangladesh |
title_short |
Aflatoxin M1 detection by ELISA in raw and processed milk in Bangladesh |
title_full |
Aflatoxin M1 detection by ELISA in raw and processed milk in Bangladesh |
title_fullStr |
Aflatoxin M1 detection by ELISA in raw and processed milk in Bangladesh |
title_full_unstemmed |
Aflatoxin M1 detection by ELISA in raw and processed milk in Bangladesh |
title_sort |
aflatoxin m1 detection by elisa in raw and processed milk in bangladesh |
publisher |
Elsevier |
series |
Toxicology Reports |
issn |
2214-7500 |
publishDate |
2020-01-01 |
description |
An analysis was accomplished to get information regarding presence of highly toxic and carcinogenic aflatoxin M1 (AFM1) in raw and processed samples of milk applying ELISA (enzyme linked immunosorbent assay). Investigation of a set of 100 samples (n=100) taken from different regional small-scale farms as well as grocery stores of Bangladesh containing three groups of milk including raw (n=50), pasteurized (n=25) and UHT (n=25), exhibited in total 53% AFM1 contamination where 70% contamination was found in raw milk ranging from 22.79−1489.28 ng/kg (mean value 699.07 ng/kg), 52% in pasteurized milk ranging from 18.11−672.18 ng/kg (mean value 99.77 ng/kg) and 20% in UHT milk ranging from 25.07−48.95 ng/kg (mean value 35.46 ng/kg). Among all the positive samples, 75% contaminated samples were above the European Communities prescribed limits (50 ng/kg) while having 25% samples still below this limit whereas 43% samples crossed the accepted limit of US regulations/Codex Alimentarius Commission regulations (500 ng/kg). Thus the findings of this study may lead to increase awareness regarding health impact of aflatoxin M1 and implementation of strict regulations by law enforcement bureaus of Bangladesh. |
topic |
Aflatoxin Aflatoxin M1 Aflatoxin B1 ELISA Milk |
url |
http://www.sciencedirect.com/science/article/pii/S2214750020304066 |
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